odyssey clx imaging system (LI-COR)
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odyssey clx imaging system
Odyssey Clx Imaging System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 90480 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imagers/Odyssey+CLx+Imaging+System/custom%409141-10%4042701686
Average 99 stars, based on 90480 article reviews
Odyssey Clx Imaging System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 90480 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imagers/Odyssey+CLx+Imaging+System/custom%409141-10%4042701686
Average 99 stars, based on 90480 article reviews
odyssey clx imaging system - by Bioz Stars,
2026-10
99/100 stars
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Western Blot:Article Title: Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems Article Snippet: The following primary antibodies were used: rabbit anti-HSF1 (Cell Signaling Technology 4356, lot 2), rabbit anti acetylated-Lysine (Cell Signaling Technology 9441, lot 16), rabbit antiEnterobacteriophage MS2 Coat Protein (Millipore-Sigma, ABE76-I, lot 3764751), rabbit anti-CRBN (Sigma-Aldrich, HPA045910, lot BE106946, Fig. 1i), rabbit anti-CRBN (clone F4I7F, Cell Signaling Technology 60312, lot 1, Fig. 4d), mouse anti-α-tubulin (Cell Signaling Technology 3873, lot 19), rabbit anti Histone 3 (Cell Signaling Technology 4499, lot 20), and rabbit anti-H3K27ac (Cell Signaling Technology 8173, lot 8). .. Western Blots were imaged on LI-COR Odyssey FC or Article Title: Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems Article Snippet: To clone pXPR_502-sgAAVS1 and pXPR_502-sgCRBN-a1, pXPR_502 was cut using Esp3I (BsmBI, Thermo Fisher Scientific, ER0452) and subjected to T4 DNA ligation with annealed oligos 2692/2693 (AAVS1) or 4684/4685 (CRBN sg-a1, which was picked from the Calabrese library set A). .. Western Blots were imaged on LI-COR Odyssey FC or Membrane:Article Title: A Novel Modulator of Resistance for Oxaliplatin-Based Therapy for Colorectal Cancer: The ESCRT Family Member VPS4A. Article Snippet: IRDye® 800CW goat anti-rabbit IgG and IRDye® 680RD donkey anti-mouse IgG secondary antibodies were used at 1:5000 dilution (LI-COR Biosciences, Cambridge, UK). .. Finally, the membrane was visualized, and images were taken using Article Title: A Novel Modulator of Resistance for Oxaliplatin-Based Therapy for Colorectal Cancer: The ESCRT Family Member VPS4A Article Snippet: IRDye ® 800CW goat anti-rabbit IgG and IRDye ® 680RD donkey anti-mouse IgG secondary antibodies were used at 1:5000 dilution (LI-COR Biosciences, Cambridge, UK). .. Finally, the membrane was visualized, and images were taken using Article Title: Protocol for the purification and analysis of nuclear UFMylated proteins Article Snippet: .. Probing of membrane with antibodies continued. a. Wash the membrane 3 × 5 min using 1× PBS-T. Option 1 : Assessment of fractionation efficiency. b. Incubate the membranes with fluorescent secondary antibodies for 1 h at 25°C in 5% milk dissolved in 1× PBS-T. Add anti-rabbit and anti-mouse secondary antibodies (dilution 1:10000). c. Wash the membrane 3 × 5 min using 1× PBS-T. d. Image the membrane using fluorescence-compatible imagers such as the Article Title: Protocol for the purification and analysis of nuclear UFMylated proteins Article Snippet: .. Option 2 : Analysis of affinity purified UFMylated proteins. e. Incubate the membranes with fluorescent secondary antibodies for 1 h at 25°C in 5% milk dissolved in PBS-T. Add anti-rabbit and anti-mouse secondary antibodies (dilution 1:10000). f. Wash the membrane 3 × 5 min using PBS-T. g. Image the membrane using fluorescence-compatible imagers such as the Fractionation:Article Title: Protocol for the purification and analysis of nuclear UFMylated proteins Article Snippet: .. Probing of membrane with antibodies continued. a. Wash the membrane 3 × 5 min using 1× PBS-T. Option 1 : Assessment of fractionation efficiency. b. Incubate the membranes with fluorescent secondary antibodies for 1 h at 25°C in 5% milk dissolved in 1× PBS-T. Add anti-rabbit and anti-mouse secondary antibodies (dilution 1:10000). c. Wash the membrane 3 × 5 min using 1× PBS-T. d. Image the membrane using fluorescence-compatible imagers such as the Fluorescence:Article Title: Protocol for the purification and analysis of nuclear UFMylated proteins Article Snippet: .. Probing of membrane with antibodies continued. a. Wash the membrane 3 × 5 min using 1× PBS-T. Option 1 : Assessment of fractionation efficiency. b. Incubate the membranes with fluorescent secondary antibodies for 1 h at 25°C in 5% milk dissolved in 1× PBS-T. Add anti-rabbit and anti-mouse secondary antibodies (dilution 1:10000). c. Wash the membrane 3 × 5 min using 1× PBS-T. d. Image the membrane using fluorescence-compatible imagers such as the Article Title: Protocol for the purification and analysis of nuclear UFMylated proteins Article Snippet: .. Option 2 : Analysis of affinity purified UFMylated proteins. e. Incubate the membranes with fluorescent secondary antibodies for 1 h at 25°C in 5% milk dissolved in PBS-T. Add anti-rabbit and anti-mouse secondary antibodies (dilution 1:10000). f. Wash the membrane 3 × 5 min using PBS-T. g. Image the membrane using fluorescence-compatible imagers such as the Affinity Purification:Article Title: Protocol for the purification and analysis of nuclear UFMylated proteins Article Snippet: .. Option 2 : Analysis of affinity purified UFMylated proteins. e. Incubate the membranes with fluorescent secondary antibodies for 1 h at 25°C in 5% milk dissolved in PBS-T. Add anti-rabbit and anti-mouse secondary antibodies (dilution 1:10000). f. Wash the membrane 3 × 5 min using PBS-T. g. Image the membrane using fluorescence-compatible imagers such as the other:Article Title: TEAD1 condensates are transcriptionally inactive storage sites on the pericentromeric heterochromatin Article Snippet: Membrane was incubated overnight at 4°C with 5% non-fat milk in TBS-T milk with primary antibodies 1:1000 anti-TEAD1, 1:1000 anti-GFP (Thermo Fisher, XH352366), 1:20,000 anti-β-actin (ABClonal, 3500100011). |