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decynium22  (MedChemExpress)


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    Structured Review

    MedChemExpress decynium22
    Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and <t>decynium22</t> as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.
    Decynium22, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 3 article reviews
    decynium22 - by Bioz Stars, 2026-03
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    Images

    1) Product Images from "Influence of YES1 Kinase and Tyrosine Phosphorylation on the Activity of OCT1"

    Article Title: Influence of YES1 Kinase and Tyrosine Phosphorylation on the Activity of OCT1

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2021.644342

    Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and decynium22 as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.
    Figure Legend Snippet: Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and decynium22 as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.

    Techniques Used: Inhibition, Sequencing, Transfection, Mutagenesis, Stable Transfection, Negative Control, Positive Control, Activity Assay, Control



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    Image Search Results


    Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and decynium22 as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.

    Journal: Frontiers in Pharmacology

    Article Title: Influence of YES1 Kinase and Tyrosine Phosphorylation on the Activity of OCT1

    doi: 10.3389/fphar.2021.644342

    Figure Lengend Snippet: Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and decynium22 as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.

    Article Snippet: Reference standards of decynium22, a positive control inhibitor, as well as the TKIs bosutinib, dasatinib, gilteritinib, ibrutinib, lapatinib, sunitinib, vandetanib, and CH6953755 were obtained from MedChemExpress (Monmouth Junction, NJ).

    Techniques: Inhibition, Sequencing, Transfection, Mutagenesis, Stable Transfection, Negative Control, Positive Control, Activity Assay, Control