Review





Similar Products

decynium22  (MedChemExpress)
94
MedChemExpress decynium22
Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and <t>decynium22</t> as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.
Decynium22, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/decynium22/Pseudoisocyanine/pmc08006202-29-3-25
Average 94 stars, based on 1 article reviews
decynium22 - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
d-22 (decynium22):1,1´-diethyl-2,2´-cyanine iodide  (Millipore)
90
Millipore d-22 (decynium22):1,1´-diethyl-2,2´-cyanine iodide
Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and <t>decynium22</t> as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.
D 22 (Decynium22):1,1´ Diethyl 2,2´ Cyanine Iodide, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/decynium22/decynium+22/pm26297536-131-7-27
Average 90 stars, based on 1 article reviews
d-22 (decynium22):1,1´-diethyl-2,2´-cyanine iodide - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
decynium22  (Millipore)
90
Millipore decynium22
Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and <t>decynium22</t> as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.
Decynium22, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/decynium22/decynium+22/pm21424617-88-17-18
Average 90 stars, based on 1 article reviews
decynium22 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
decynium22 (1,1′-diethyl-2,2′-cyanine iodide  (Thermo Fisher)
90
Thermo Fisher decynium22 (1,1′-diethyl-2,2′-cyanine iodide
Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and <t>decynium22</t> as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.
Decynium22 (1,1′ Diethyl 2,2′ Cyanine Iodide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/decynium22/pm17689566-45-0-6
Average 90 stars, based on 1 article reviews
decynium22 (1,1′-diethyl-2,2′-cyanine iodide - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
decynium22  (Arbec Forest Products Inc)
90
Arbec Forest Products Inc decynium22
Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and <t>decynium22</t> as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.
Decynium22, supplied by Arbec Forest Products Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/decynium22/decynium22/pm17689566-150-7-16
Average 90 stars, based on 1 article reviews
decynium22 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
1,1¢-diethyl-2,2¢-cyanine iodide (decynium22; d22  (Millipore)
90
Millipore 1,1¢-diethyl-2,2¢-cyanine iodide (decynium22; d22
Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and <t>decynium22</t> as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.
1,1¢ Diethyl 2,2¢ Cyanine Iodide (Decynium22; D22, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/decynium22/decynium+22/pm12485400-68-3-34
Average 90 stars, based on 1 article reviews
1,1¢-diethyl-2,2¢-cyanine iodide (decynium22; d22 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
1,1p-diethyl-2,2p-cyanine iodide (decynium22  (Millipore)
90
Millipore 1,1p-diethyl-2,2p-cyanine iodide (decynium22
Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and <t>decynium22</t> as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.
1,1p Diethyl 2,2p Cyanine Iodide (Decynium22, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/decynium22/decynium+22/pm10082798-31-0-5
Average 90 stars, based on 1 article reviews
1,1p-diethyl-2,2p-cyanine iodide (decynium22 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and decynium22 as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.

Journal: Frontiers in Pharmacology

Article Title: Influence of YES1 Kinase and Tyrosine Phosphorylation on the Activity of OCT1

doi: 10.3389/fphar.2021.644342

Figure Lengend Snippet: Inhibition of organic cation transporters by TKIs. (A) The protein sequence of hOCT1, hOCT2, and hOCT3 was aligned by a multiple sequence alignment program (MAFFT). (B) OCT1 protein sequence from indicated organisms was aligned by a multiple sequence alignment program (MAFFT). (C) HEK293 cells were transiently transfected with wild-type (WT), Y240F, Y361F, and Y376F mutant plasmids, uptake assays were performed using [ 14 C] TEA (2 µM) for 15 min. Cellular accumulation of [ 14 C] TEA was determined by liquid scintillation counter, and the graph represents relative uptake values compared to wild-type after normalization of protein levels. (D) Relative transporter function in HEK293 cells stably transfected with hOCT1 was evaluated by a substrate drug TEA in the presence of FDA-approved TKIs (10 µM) previously found to inhibit OCT2. Lapatinib was included as a negative-control TKI, and decynium22 as a non-TKI positive control inhibitor. The graph represents relative transport activity of indicated substrate drug compared to DMSO. * p < 0.05 vs. wild-type control. All values represent mean ± SEM.

Article Snippet: Reference standards of decynium22, a positive control inhibitor, as well as the TKIs bosutinib, dasatinib, gilteritinib, ibrutinib, lapatinib, sunitinib, vandetanib, and CH6953755 were obtained from MedChemExpress (Monmouth Junction, NJ).

Techniques: Inhibition, Sequencing, Transfection, Mutagenesis, Stable Transfection, Negative Control, Positive Control, Activity Assay, Control