one-color microarray-based gene expression analysis protocol (Agilent technologies)
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Agilent technologies
one-color microarray-based gene expression analysis protocol
One Color Microarray Based Gene Expression Analysis Protocol, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray-based+gene+expression+analysis+protocol/pmc06921299-55-11-10
Average 90 stars, based on 1 article reviews
One Color Microarray Based Gene Expression Analysis Protocol, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray-based+gene+expression+analysis+protocol/pmc06921299-55-11-10
Average 90 stars, based on 1 article reviews
one-color microarray-based gene expression analysis protocol - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "LncRNA PICSAR promotes cell proliferation, migration and invasion of fibroblast-like synoviocytes by sponging miRNA-4701-5p in rheumatoid arthritis"
Article Title: LncRNA PICSAR promotes cell proliferation, migration and invasion of fibroblast-like synoviocytes by sponging miRNA-4701-5p in rheumatoid arthritis
Journal: EBioMedicine
doi: 10.1016/j.ebiom.2019.11.024
Figure Legend Snippet: Expression Profiles of differently expressed lncRNAs and mRNAs screened by microarray analysis of fibroblast-like synoviocytes (FLSs) samples from rheumatoid arthritis (RA) group ( n = 3) and control group ( n = 3). With the threshold of fold changes > 1.5 and P < 0.05, (a) the Volcano Plot showed differentially expressed lncRNAs between RA-FLSs and HC-FLSs and (b) the Scatter Plot showed differentially expressed mRNAs. (c) The Heat Map showing distinguishable lncRNA expression profiles with fold changes > 2.5 and P < 0.05.
Techniques Used: Expressing, Microarray
Figure Legend Snippet: Verification of lncRNAs and effect of lncRNA PICSAR suppression on the proliferation of rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs). (a) Quantitative real-time reverse transcription PCR (qRT-PCR) verified 5 selected lncRNAs with the highest up-regulated differential expression according to the microarray analysis. LncRNA PICSAR is the most significantly up-regulated in RA-FLSs. (b) LncRNA PICSAR was also over-expressed in the synovial fluid of RA patients compared with the control group by qPCR. (c) The inhibition efficiency of specific PICSAR small interference RNA (siRNA) in RA-FLSs was detected by qRT-PCR. (d) Compared with negative control, the expression of p-ERK1/2 in the total ERK1/2 protein was significantly decreased in the RA-FLSs after transfection. (e) PICSAR knockdown in RA-FLSs decreases cell proliferation assessed by a Cell Counting Kit-8 assay. The absorbance at 450 nm wavelength of PICSAR-siRNA group were significantly reduced at the indicated time points compared to the other two control groups. All the results were presented as the mean ± standard deviation (S.D.) based on ≥ 3 replicates involving ≥ 3 samples. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus control groups.
Techniques Used: Quantitative RT-PCR, Expressing, Microarray, Inhibition, Negative Control, Transfection, Cell Counting, Standard Deviation
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from Hypospadias Patients Article Snippet: Article Title: Intratracheal Transplantation of Amnion-Derived Mesenchymal Stem Cells Ameliorates Hyperoxia-Induced Neonatal Hyperoxic Lung Injury via Aminoacyl-Peptide Hydrolase Article Snippet: RNA labeling and hybridization were performed according to the Agilent One-Color Microarray-Based Gene Ex pression Analysis protocol ( Article Title: Development and validation of a gene expression oligo microarray for the gilthead sea bream ( Sparus aurata ) Article Snippet: Article Title: Evaluation of phenoxybenzamine in the CFA model of pain following gene expression studies and connectivity mapping Article Snippet: Sample preparation, hybridization, wash, scanning and feature extraction was performed according to the standard protocol, ' Article Title: Comparison of host cell gene expression in cowpox, monkeypox or vaccinia virus-infected cells reveals virus-specific regulation of immune response genes Article Snippet: Sample labelling was performed as detailed in the Article Title: Development of an oligo DNA microarray for the European sea bass and its application to expression profiling of jaw deformity Article Snippet: |
