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microarray-based gene expression analysis protocol  (Arraystar inc)

 
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    Arraystar inc microarray-based gene expression analysis protocol
    Microarray Based Gene Expression Analysis Protocol, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray-based+gene+expression+analysis+protocol/gene+expression+microarray+analysis/pm39096478-76-13-13
    Average 90 stars, based on 1 article reviews
    microarray-based gene expression analysis protocol - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Infection:

    Article Title: HCV-Associated Exosomes Upregulate RUNXOR and RUNX1 Expressions to Promote MDSC Expansion and Suppressive Functions through STAT3–miR124 Axis
    Article Snippet: .. To determine whether lncRNAs play a role in MDSC expansion during HCV infection, we analyzed the transcripts of lncRNAs and messenger RNAs (mRNAs) in MDSCs isolated from HCV-infected individuals and HS using the Arraystar gene expression array. ..

    Isolation:

    Article Title: HCV-Associated Exosomes Upregulate RUNXOR and RUNX1 Expressions to Promote MDSC Expansion and Suppressive Functions through STAT3–miR124 Axis
    Article Snippet: .. To determine whether lncRNAs play a role in MDSC expansion during HCV infection, we analyzed the transcripts of lncRNAs and messenger RNAs (mRNAs) in MDSCs isolated from HCV-infected individuals and HS using the Arraystar gene expression array. ..

    Article Title: Telomeric injury by KML001 in human T cells induces mitochondrial dysfunction through the p53-PGC-1α pathway
    Article Snippet: .. CD4 T cells were isolated from HS and cultured with 5 μM KML001 or DPBS control for 48 h. Approximately 6 × 10 6 cells per group were sent to Arraystar Inc. (Rockville, MD) for gene expression analysis. ..

    Gene Expression:

    Article Title: HCV-Associated Exosomes Upregulate RUNXOR and RUNX1 Expressions to Promote MDSC Expansion and Suppressive Functions through STAT3–miR124 Axis
    Article Snippet: .. To determine whether lncRNAs play a role in MDSC expansion during HCV infection, we analyzed the transcripts of lncRNAs and messenger RNAs (mRNAs) in MDSCs isolated from HCV-infected individuals and HS using the Arraystar gene expression array. ..

    Article Title: Histone acetyltransferase 7 (KAT7)-dependent intragenic histone acetylation regulates endothelial cell gene regulation
    Article Snippet: .. Gene expression microarray analysis Total RNA of HUVEC samples treated with control siRNA or KAT7 siRNAs ( n = 4) were processed by Arraystar Inc. for gene expression microarray analysis. .. The microarray used for sample hybridization is the human LncRNA microarray V3.0, a custom 8 × 60K Agilent array containing 58,944 probes that detect 26,109 mRNA transcripts and 30,586 long non-coding RNA transcripts.

    Article Title: Mitochondrial Functions Are Compromised in CD4 T Cells From ART-Controlled PLHIV
    Article Snippet: .. The gene expression analysis was performed by Arraystar Inc (Rockville, MD) and the heat map was generated using an online heatmapper software following the Average linkage clustering and Euclidean distance measurement methods ( ). ..

    Article Title: Telomeric injury by KML001 in human T cells induces mitochondrial dysfunction through the p53-PGC-1α pathway
    Article Snippet: .. CD4 T cells were isolated from HS and cultured with 5 μM KML001 or DPBS control for 48 h. Approximately 6 × 10 6 cells per group were sent to Arraystar Inc. (Rockville, MD) for gene expression analysis. ..

    Article Title: Telomere and ATM Dynamics in CD4 T-Cell Depletion in Active and Virus-Suppressed HIV Infections
    Article Snippet: .. Gene expression analysis in HIV-infected and 420 uninfected SupT1 cells and primary CD4 T cells was performed by Arraystar (Arraystar Inc, Rockville, MD). ..

    Microarray:

    Article Title: Histone acetyltransferase 7 (KAT7)-dependent intragenic histone acetylation regulates endothelial cell gene regulation
    Article Snippet: .. Gene expression microarray analysis Total RNA of HUVEC samples treated with control siRNA or KAT7 siRNAs ( n = 4) were processed by Arraystar Inc. for gene expression microarray analysis. .. The microarray used for sample hybridization is the human LncRNA microarray V3.0, a custom 8 × 60K Agilent array containing 58,944 probes that detect 26,109 mRNA transcripts and 30,586 long non-coding RNA transcripts.

    Control:

    Article Title: Histone acetyltransferase 7 (KAT7)-dependent intragenic histone acetylation regulates endothelial cell gene regulation
    Article Snippet: .. Gene expression microarray analysis Total RNA of HUVEC samples treated with control siRNA or KAT7 siRNAs ( n = 4) were processed by Arraystar Inc. for gene expression microarray analysis. .. The microarray used for sample hybridization is the human LncRNA microarray V3.0, a custom 8 × 60K Agilent array containing 58,944 probes that detect 26,109 mRNA transcripts and 30,586 long non-coding RNA transcripts.

    Article Title: Telomeric injury by KML001 in human T cells induces mitochondrial dysfunction through the p53-PGC-1α pathway
    Article Snippet: .. CD4 T cells were isolated from HS and cultured with 5 μM KML001 or DPBS control for 48 h. Approximately 6 × 10 6 cells per group were sent to Arraystar Inc. (Rockville, MD) for gene expression analysis. ..

    other:

    Article Title: MUC20 regulated by extrachromosomal circular DNA attenuates proteasome inhibitor resistance of multiple myeloma by modulating cuproptosis
    Article Snippet: Total RNA was extracted from KAS-6/1 and U266 cells and sent to Aksomics (Shanghai, China) for RNA microarray analysis using the ArrayStar Gene Expression Array (Human, 4 × 44 K, Rockville, MD, USA).

    Article Title: Synergistic Effects of Glutamine Deprivation and Metformin in Acute Myeloid Leukemia.
    Article Snippet: [Abstract] Objective: The metabolic reprogramming of acute myeloid leukemia (AML) cells is a compensatory adaptation to meet energy requirements for rapid proliferation.. This study aimed to examine the synergistic effects of glutamine deprivation and metformin exposure on AML cells.. Methods: SKM-1 cells (an AML cell line) were subjected to glutamine deprivation and/or treatment with metformin or bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl) ethyl sulfide (BPTES, a glutaminase inhibitor) or cytarabine.

    Article Title: Transcriptome analysis of life stages of the house cricket, Acheta domesticus, to improve insect crop production.
    Article Snippet: Gene expression in each life stage was analyzed by ArrayStar (DNAStar).

    Generated:

    Article Title: Mitochondrial Functions Are Compromised in CD4 T Cells From ART-Controlled PLHIV
    Article Snippet: .. The gene expression analysis was performed by Arraystar Inc (Rockville, MD) and the heat map was generated using an online heatmapper software following the Average linkage clustering and Euclidean distance measurement methods ( ). ..

    Software:

    Article Title: Mitochondrial Functions Are Compromised in CD4 T Cells From ART-Controlled PLHIV
    Article Snippet: .. The gene expression analysis was performed by Arraystar Inc (Rockville, MD) and the heat map was generated using an online heatmapper software following the Average linkage clustering and Euclidean distance measurement methods ( ). ..

    Cell Culture:

    Article Title: Telomeric injury by KML001 in human T cells induces mitochondrial dysfunction through the p53-PGC-1α pathway
    Article Snippet: .. CD4 T cells were isolated from HS and cultured with 5 μM KML001 or DPBS control for 48 h. Approximately 6 × 10 6 cells per group were sent to Arraystar Inc. (Rockville, MD) for gene expression analysis. ..



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    Image Search Results


    Significant genes of each signature were represented according to their gene function and specific role in the cell, localization and type of interaction between them. Node border color refers to cell localization, node shape to general function and node color to specific function in the cell. Edge color refers to physical interactions, biochemical interactions or to both; when not specified, a functional interaction is assumed. Upstream arrow (red) means up-regulation versus the other categories, and downstream arrow (blue) means down-regulation versus the other categories. Microarray data values represented here are shown in and .

    Journal: PLoS ONE

    Article Title: Defining the Genomic Signature of Totipotency and Pluripotency during Early Human Development

    doi: 10.1371/journal.pone.0062135

    Figure Lengend Snippet: Significant genes of each signature were represented according to their gene function and specific role in the cell, localization and type of interaction between them. Node border color refers to cell localization, node shape to general function and node color to specific function in the cell. Edge color refers to physical interactions, biochemical interactions or to both; when not specified, a functional interaction is assumed. Upstream arrow (red) means up-regulation versus the other categories, and downstream arrow (blue) means down-regulation versus the other categories. Microarray data values represented here are shown in and .

    Article Snippet: Following ‘One-Color Microarray-Based Gene Expression Analysis’ protocol Version 5.7 (Agilent p/n G4140-90040), 3 µg of labelled cRNA was hybridised with the Whole Human Genome Oligo Microarray Kit (Agilent p/n G2519F-014850) containing 41,000+ unique human genes and transcripts.

    Techniques: Functional Assay, Microarray

    Expression Profiles of differently expressed lncRNAs and mRNAs screened by microarray analysis of fibroblast-like synoviocytes (FLSs) samples from rheumatoid arthritis (RA) group ( n = 3) and control group ( n = 3). With the threshold of fold changes > 1.5 and P < 0.05, (a) the Volcano Plot showed differentially expressed lncRNAs between RA-FLSs and HC-FLSs and (b) the Scatter Plot showed differentially expressed mRNAs. (c) The Heat Map showing distinguishable lncRNA expression profiles with fold changes > 2.5 and P < 0.05.

    Journal: EBioMedicine

    Article Title: LncRNA PICSAR promotes cell proliferation, migration and invasion of fibroblast-like synoviocytes by sponging miRNA-4701-5p in rheumatoid arthritis

    doi: 10.1016/j.ebiom.2019.11.024

    Figure Lengend Snippet: Expression Profiles of differently expressed lncRNAs and mRNAs screened by microarray analysis of fibroblast-like synoviocytes (FLSs) samples from rheumatoid arthritis (RA) group ( n = 3) and control group ( n = 3). With the threshold of fold changes > 1.5 and P < 0.05, (a) the Volcano Plot showed differentially expressed lncRNAs between RA-FLSs and HC-FLSs and (b) the Scatter Plot showed differentially expressed mRNAs. (c) The Heat Map showing distinguishable lncRNA expression profiles with fold changes > 2.5 and P < 0.05.

    Article Snippet: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology) with minor modifications.

    Techniques: Expressing, Microarray

    Verification of lncRNAs and effect of lncRNA PICSAR suppression on the proliferation of rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs). (a) Quantitative real-time reverse transcription PCR (qRT-PCR) verified 5 selected lncRNAs with the highest up-regulated differential expression according to the microarray analysis. LncRNA PICSAR is the most significantly up-regulated in RA-FLSs. (b) LncRNA PICSAR was also over-expressed in the synovial fluid of RA patients compared with the control group by qPCR. (c) The inhibition efficiency of specific PICSAR small interference RNA (siRNA) in RA-FLSs was detected by qRT-PCR. (d) Compared with negative control, the expression of p-ERK1/2 in the total ERK1/2 protein was significantly decreased in the RA-FLSs after transfection. (e) PICSAR knockdown in RA-FLSs decreases cell proliferation assessed by a Cell Counting Kit-8 assay. The absorbance at 450 nm wavelength of PICSAR-siRNA group were significantly reduced at the indicated time points compared to the other two control groups. All the results were presented as the mean ± standard deviation (S.D.) based on ≥ 3 replicates involving ≥ 3 samples. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus control groups.

    Journal: EBioMedicine

    Article Title: LncRNA PICSAR promotes cell proliferation, migration and invasion of fibroblast-like synoviocytes by sponging miRNA-4701-5p in rheumatoid arthritis

    doi: 10.1016/j.ebiom.2019.11.024

    Figure Lengend Snippet: Verification of lncRNAs and effect of lncRNA PICSAR suppression on the proliferation of rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs). (a) Quantitative real-time reverse transcription PCR (qRT-PCR) verified 5 selected lncRNAs with the highest up-regulated differential expression according to the microarray analysis. LncRNA PICSAR is the most significantly up-regulated in RA-FLSs. (b) LncRNA PICSAR was also over-expressed in the synovial fluid of RA patients compared with the control group by qPCR. (c) The inhibition efficiency of specific PICSAR small interference RNA (siRNA) in RA-FLSs was detected by qRT-PCR. (d) Compared with negative control, the expression of p-ERK1/2 in the total ERK1/2 protein was significantly decreased in the RA-FLSs after transfection. (e) PICSAR knockdown in RA-FLSs decreases cell proliferation assessed by a Cell Counting Kit-8 assay. The absorbance at 450 nm wavelength of PICSAR-siRNA group were significantly reduced at the indicated time points compared to the other two control groups. All the results were presented as the mean ± standard deviation (S.D.) based on ≥ 3 replicates involving ≥ 3 samples. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus control groups.

    Article Snippet: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology) with minor modifications.

    Techniques: Quantitative RT-PCR, Expressing, Microarray, Inhibition, Negative Control, Transfection, Cell Counting, Standard Deviation