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‘one-color microarray-based gene expression analysis’ protocol  (Agilent technologies)


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    Agilent technologies ‘one-color microarray-based gene expression analysis’ protocol
    ‘One Color Microarray Based Gene Expression Analysis’ Protocol, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray-based+gene+expression+analysis+protocol/pm24595438-229-6-29
    Average 90 stars, based on 1 article reviews
    ‘one-color microarray-based gene expression analysis’ protocol - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Labeling:

    Article Title: Analysis of Lsm Protein-Mediated Regulation in the Haloarchaeon Haloferax mediterranei .
    Article Snippet: The cDNA labeling was performed with the two-color microarray-based gene expression analysis v. 6.5 (Agilent Technologies, CA, USA) according to the manufacturer’s protocol.

    Article Title: Maimendong and Qianjinweijing Tang combined with cisplatin suppressed lung cancer through targeting lncRNA-p21.
    Article Snippet: Ethnopharmacological relevance: Maimendong and Qianjinweijing Tang (Jin formula) is a traditional Chinese medicine formula that has been proven effective in the treatment of lung cancer in long-term clinical practice.. Aim of the study: To evaluate the anti-tumor effects of Jin formula combined with cisplatin (JIN + DDP) in vivo and in vitro, as well as to explore the role of long non-coding RNA (lncRNA) in the anti-lung cancer mechanism of

    Article Title: Heterozygous NF1 dermal fibroblasts modulate exosomal content to promote angiogenesis in a tissue-engineered skin model of neurofibromatosis type-1.
    Article Snippet: Gene expression was assessed using the One-Color Microarray-Based Gene Expression Analysis kit (Agilent Technologies) as previously described (Roy et al., 2022).

    Article Title: Tumor-derived exosomes RNA expression profiling identifies the prognosis, immune characteristics, and treatment in HR+/HER2-breast cancer
    Article Snippet: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-based Gene Expression Analysis protocol (Agilent Technologies).

    Article Title: Oncopig bladder cancer cells recapitulate human bladder cancer treatment responses in vitro
    Article Snippet: To obtain the gene transcription panel of treated and untreated human and Oncopig cells, the Two-Color Microarray-Based Gene Expression Analysis microarrays (Agilent Technologies Inc, Santa Clara, CA, USA) were used according to the manufacturer’s instructions.

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy.
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell.
    Article Snippet: RNA labeling and hybridization were performed according to the manufacturer’s instructions (Agilent “One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp labeling)”).

    Hybridization:

    Article Title: Analysis of Lsm Protein-Mediated Regulation in the Haloarchaeon Haloferax mediterranei .
    Article Snippet: The cDNA labeling was performed with the two-color microarray-based gene expression analysis v. 6.5 (Agilent Technologies, CA, USA) according to the manufacturer’s protocol.

    Article Title: Maimendong and Qianjinweijing Tang combined with cisplatin suppressed lung cancer through targeting lncRNA-p21.
    Article Snippet: Ethnopharmacological relevance: Maimendong and Qianjinweijing Tang (Jin formula) is a traditional Chinese medicine formula that has been proven effective in the treatment of lung cancer in long-term clinical practice.. Aim of the study: To evaluate the anti-tumor effects of Jin formula combined with cisplatin (JIN + DDP) in vivo and in vitro, as well as to explore the role of long non-coding RNA (lncRNA) in the anti-lung cancer mechanism of

    Article Title: Heterozygous NF1 dermal fibroblasts modulate exosomal content to promote angiogenesis in a tissue-engineered skin model of neurofibromatosis type-1.
    Article Snippet: Gene expression was assessed using the One-Color Microarray-Based Gene Expression Analysis kit (Agilent Technologies) as previously described (Roy et al., 2022).

    Article Title: Tumor-derived exosomes RNA expression profiling identifies the prognosis, immune characteristics, and treatment in HR+/HER2-breast cancer
    Article Snippet: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-based Gene Expression Analysis protocol (Agilent Technologies).

    Article Title: Oncopig bladder cancer cells recapitulate human bladder cancer treatment responses in vitro
    Article Snippet: To obtain the gene transcription panel of treated and untreated human and Oncopig cells, the Two-Color Microarray-Based Gene Expression Analysis microarrays (Agilent Technologies Inc, Santa Clara, CA, USA) were used according to the manufacturer’s instructions.

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy.
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell.
    Article Snippet: RNA labeling and hybridization were performed according to the manufacturer’s instructions (Agilent “One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp labeling)”).

    Microarray:

    Article Title: Analysis of Lsm Protein-Mediated Regulation in the Haloarchaeon Haloferax mediterranei .
    Article Snippet: The cDNA labeling was performed with the two-color microarray-based gene expression analysis v. 6.5 (Agilent Technologies, CA, USA) according to the manufacturer’s protocol.

    Article Title: Maimendong and Qianjinweijing Tang combined with cisplatin suppressed lung cancer through targeting lncRNA-p21.
    Article Snippet: Ethnopharmacological relevance: Maimendong and Qianjinweijing Tang (Jin formula) is a traditional Chinese medicine formula that has been proven effective in the treatment of lung cancer in long-term clinical practice.. Aim of the study: To evaluate the anti-tumor effects of Jin formula combined with cisplatin (JIN + DDP) in vivo and in vitro, as well as to explore the role of long non-coding RNA (lncRNA) in the anti-lung cancer mechanism of

    Article Title: Heterozygous NF1 dermal fibroblasts modulate exosomal content to promote angiogenesis in a tissue-engineered skin model of neurofibromatosis type-1.
    Article Snippet: Gene expression was assessed using the One-Color Microarray-Based Gene Expression Analysis kit (Agilent Technologies) as previously described (Roy et al., 2022).

    Article Title: Tumor-derived exosomes RNA expression profiling identifies the prognosis, immune characteristics, and treatment in HR+/HER2-breast cancer
    Article Snippet: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-based Gene Expression Analysis protocol (Agilent Technologies).

    Article Title: Oncopig bladder cancer cells recapitulate human bladder cancer treatment responses in vitro
    Article Snippet: To obtain the gene transcription panel of treated and untreated human and Oncopig cells, the Two-Color Microarray-Based Gene Expression Analysis microarrays (Agilent Technologies Inc, Santa Clara, CA, USA) were used according to the manufacturer’s instructions.

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy.
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell.
    Article Snippet: RNA labeling and hybridization were performed according to the manufacturer’s instructions (Agilent “One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp labeling)”).

    Expressing:

    Article Title: Analysis of Lsm Protein-Mediated Regulation in the Haloarchaeon Haloferax mediterranei .
    Article Snippet: The cDNA labeling was performed with the two-color microarray-based gene expression analysis v. 6.5 (Agilent Technologies, CA, USA) according to the manufacturer’s protocol.

    Article Title: Maimendong and Qianjinweijing Tang combined with cisplatin suppressed lung cancer through targeting lncRNA-p21.
    Article Snippet: Ethnopharmacological relevance: Maimendong and Qianjinweijing Tang (Jin formula) is a traditional Chinese medicine formula that has been proven effective in the treatment of lung cancer in long-term clinical practice.. Aim of the study: To evaluate the anti-tumor effects of Jin formula combined with cisplatin (JIN + DDP) in vivo and in vitro, as well as to explore the role of long non-coding RNA (lncRNA) in the anti-lung cancer mechanism of

    Article Title: Heterozygous NF1 dermal fibroblasts modulate exosomal content to promote angiogenesis in a tissue-engineered skin model of neurofibromatosis type-1.
    Article Snippet: Gene expression was assessed using the One-Color Microarray-Based Gene Expression Analysis kit (Agilent Technologies) as previously described (Roy et al., 2022).

    Article Title: Tumor-derived exosomes RNA expression profiling identifies the prognosis, immune characteristics, and treatment in HR+/HER2-breast cancer
    Article Snippet: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-based Gene Expression Analysis protocol (Agilent Technologies).

    Article Title: Oncopig bladder cancer cells recapitulate human bladder cancer treatment responses in vitro
    Article Snippet: To obtain the gene transcription panel of treated and untreated human and Oncopig cells, the Two-Color Microarray-Based Gene Expression Analysis microarrays (Agilent Technologies Inc, Santa Clara, CA, USA) were used according to the manufacturer’s instructions.

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy.
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell.
    Article Snippet: RNA labeling and hybridization were performed according to the manufacturer’s instructions (Agilent “One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp labeling)”).

    Activation Assay:

    Article Title: Analysis of Lsm Protein-Mediated Regulation in the Haloarchaeon Haloferax mediterranei .
    Article Snippet: The cDNA labeling was performed with the two-color microarray-based gene expression analysis v. 6.5 (Agilent Technologies, CA, USA) according to the manufacturer’s protocol.

    Article Title: Maimendong and Qianjinweijing Tang combined with cisplatin suppressed lung cancer through targeting lncRNA-p21.
    Article Snippet: Ethnopharmacological relevance: Maimendong and Qianjinweijing Tang (Jin formula) is a traditional Chinese medicine formula that has been proven effective in the treatment of lung cancer in long-term clinical practice.. Aim of the study: To evaluate the anti-tumor effects of Jin formula combined with cisplatin (JIN + DDP) in vivo and in vitro, as well as to explore the role of long non-coding RNA (lncRNA) in the anti-lung cancer mechanism of

    Article Title: Heterozygous NF1 dermal fibroblasts modulate exosomal content to promote angiogenesis in a tissue-engineered skin model of neurofibromatosis type-1.
    Article Snippet: Gene expression was assessed using the One-Color Microarray-Based Gene Expression Analysis kit (Agilent Technologies) as previously described (Roy et al., 2022).

    Article Title: Tumor-derived exosomes RNA expression profiling identifies the prognosis, immune characteristics, and treatment in HR+/HER2-breast cancer
    Article Snippet: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-based Gene Expression Analysis protocol (Agilent Technologies).

    Article Title: Oncopig bladder cancer cells recapitulate human bladder cancer treatment responses in vitro
    Article Snippet: To obtain the gene transcription panel of treated and untreated human and Oncopig cells, the Two-Color Microarray-Based Gene Expression Analysis microarrays (Agilent Technologies Inc, Santa Clara, CA, USA) were used according to the manufacturer’s instructions.

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy.
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell.
    Article Snippet: RNA labeling and hybridization were performed according to the manufacturer’s instructions (Agilent “One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp labeling)”).

    Isolation:

    Article Title: Analysis of Lsm Protein-Mediated Regulation in the Haloarchaeon Haloferax mediterranei .
    Article Snippet: The cDNA labeling was performed with the two-color microarray-based gene expression analysis v. 6.5 (Agilent Technologies, CA, USA) according to the manufacturer’s protocol.

    Article Title: Maimendong and Qianjinweijing Tang combined with cisplatin suppressed lung cancer through targeting lncRNA-p21.
    Article Snippet: Ethnopharmacological relevance: Maimendong and Qianjinweijing Tang (Jin formula) is a traditional Chinese medicine formula that has been proven effective in the treatment of lung cancer in long-term clinical practice.. Aim of the study: To evaluate the anti-tumor effects of Jin formula combined with cisplatin (JIN + DDP) in vivo and in vitro, as well as to explore the role of long non-coding RNA (lncRNA) in the anti-lung cancer mechanism of

    Article Title: Heterozygous NF1 dermal fibroblasts modulate exosomal content to promote angiogenesis in a tissue-engineered skin model of neurofibromatosis type-1.
    Article Snippet: Gene expression was assessed using the One-Color Microarray-Based Gene Expression Analysis kit (Agilent Technologies) as previously described (Roy et al., 2022).

    Article Title: Tumor-derived exosomes RNA expression profiling identifies the prognosis, immune characteristics, and treatment in HR+/HER2-breast cancer
    Article Snippet: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-based Gene Expression Analysis protocol (Agilent Technologies).

    Article Title: Oncopig bladder cancer cells recapitulate human bladder cancer treatment responses in vitro
    Article Snippet: To obtain the gene transcription panel of treated and untreated human and Oncopig cells, the Two-Color Microarray-Based Gene Expression Analysis microarrays (Agilent Technologies Inc, Santa Clara, CA, USA) were used according to the manufacturer’s instructions.

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy.
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell.
    Article Snippet: RNA labeling and hybridization were performed according to the manufacturer’s instructions (Agilent “One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp labeling)”).

    Injection:

    Article Title: Analysis of Lsm Protein-Mediated Regulation in the Haloarchaeon Haloferax mediterranei .
    Article Snippet: The cDNA labeling was performed with the two-color microarray-based gene expression analysis v. 6.5 (Agilent Technologies, CA, USA) according to the manufacturer’s protocol.

    Article Title: Maimendong and Qianjinweijing Tang combined with cisplatin suppressed lung cancer through targeting lncRNA-p21.
    Article Snippet: Ethnopharmacological relevance: Maimendong and Qianjinweijing Tang (Jin formula) is a traditional Chinese medicine formula that has been proven effective in the treatment of lung cancer in long-term clinical practice.. Aim of the study: To evaluate the anti-tumor effects of Jin formula combined with cisplatin (JIN + DDP) in vivo and in vitro, as well as to explore the role of long non-coding RNA (lncRNA) in the anti-lung cancer mechanism of

    Article Title: Heterozygous NF1 dermal fibroblasts modulate exosomal content to promote angiogenesis in a tissue-engineered skin model of neurofibromatosis type-1.
    Article Snippet: Gene expression was assessed using the One-Color Microarray-Based Gene Expression Analysis kit (Agilent Technologies) as previously described (Roy et al., 2022).

    Article Title: Tumor-derived exosomes RNA expression profiling identifies the prognosis, immune characteristics, and treatment in HR+/HER2-breast cancer
    Article Snippet: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-based Gene Expression Analysis protocol (Agilent Technologies).

    Article Title: Oncopig bladder cancer cells recapitulate human bladder cancer treatment responses in vitro
    Article Snippet: To obtain the gene transcription panel of treated and untreated human and Oncopig cells, the Two-Color Microarray-Based Gene Expression Analysis microarrays (Agilent Technologies Inc, Santa Clara, CA, USA) were used according to the manufacturer’s instructions.

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy.
    Article Snippet: Sample labeling and array hybridisation were performed with Agilent One-Color Microarray-Based Gene Expression Analysis and miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Palo Alto CA, USA).

    Article Title: TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell.
    Article Snippet: RNA labeling and hybridization were performed according to the manufacturer’s instructions (Agilent “One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp labeling)”).



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    Image Search Results


    Significant genes of each signature were represented according to their gene function and specific role in the cell, localization and type of interaction between them. Node border color refers to cell localization, node shape to general function and node color to specific function in the cell. Edge color refers to physical interactions, biochemical interactions or to both; when not specified, a functional interaction is assumed. Upstream arrow (red) means up-regulation versus the other categories, and downstream arrow (blue) means down-regulation versus the other categories. Microarray data values represented here are shown in and .

    Journal: PLoS ONE

    Article Title: Defining the Genomic Signature of Totipotency and Pluripotency during Early Human Development

    doi: 10.1371/journal.pone.0062135

    Figure Lengend Snippet: Significant genes of each signature were represented according to their gene function and specific role in the cell, localization and type of interaction between them. Node border color refers to cell localization, node shape to general function and node color to specific function in the cell. Edge color refers to physical interactions, biochemical interactions or to both; when not specified, a functional interaction is assumed. Upstream arrow (red) means up-regulation versus the other categories, and downstream arrow (blue) means down-regulation versus the other categories. Microarray data values represented here are shown in and .

    Article Snippet: Following ‘One-Color Microarray-Based Gene Expression Analysis’ protocol Version 5.7 (Agilent p/n G4140-90040), 3 µg of labelled cRNA was hybridised with the Whole Human Genome Oligo Microarray Kit (Agilent p/n G2519F-014850) containing 41,000+ unique human genes and transcripts.

    Techniques: Functional Assay, Microarray

    Expression Profiles of differently expressed lncRNAs and mRNAs screened by microarray analysis of fibroblast-like synoviocytes (FLSs) samples from rheumatoid arthritis (RA) group ( n = 3) and control group ( n = 3). With the threshold of fold changes > 1.5 and P < 0.05, (a) the Volcano Plot showed differentially expressed lncRNAs between RA-FLSs and HC-FLSs and (b) the Scatter Plot showed differentially expressed mRNAs. (c) The Heat Map showing distinguishable lncRNA expression profiles with fold changes > 2.5 and P < 0.05.

    Journal: EBioMedicine

    Article Title: LncRNA PICSAR promotes cell proliferation, migration and invasion of fibroblast-like synoviocytes by sponging miRNA-4701-5p in rheumatoid arthritis

    doi: 10.1016/j.ebiom.2019.11.024

    Figure Lengend Snippet: Expression Profiles of differently expressed lncRNAs and mRNAs screened by microarray analysis of fibroblast-like synoviocytes (FLSs) samples from rheumatoid arthritis (RA) group ( n = 3) and control group ( n = 3). With the threshold of fold changes > 1.5 and P < 0.05, (a) the Volcano Plot showed differentially expressed lncRNAs between RA-FLSs and HC-FLSs and (b) the Scatter Plot showed differentially expressed mRNAs. (c) The Heat Map showing distinguishable lncRNA expression profiles with fold changes > 2.5 and P < 0.05.

    Article Snippet: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology) with minor modifications.

    Techniques: Expressing, Microarray

    Verification of lncRNAs and effect of lncRNA PICSAR suppression on the proliferation of rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs). (a) Quantitative real-time reverse transcription PCR (qRT-PCR) verified 5 selected lncRNAs with the highest up-regulated differential expression according to the microarray analysis. LncRNA PICSAR is the most significantly up-regulated in RA-FLSs. (b) LncRNA PICSAR was also over-expressed in the synovial fluid of RA patients compared with the control group by qPCR. (c) The inhibition efficiency of specific PICSAR small interference RNA (siRNA) in RA-FLSs was detected by qRT-PCR. (d) Compared with negative control, the expression of p-ERK1/2 in the total ERK1/2 protein was significantly decreased in the RA-FLSs after transfection. (e) PICSAR knockdown in RA-FLSs decreases cell proliferation assessed by a Cell Counting Kit-8 assay. The absorbance at 450 nm wavelength of PICSAR-siRNA group were significantly reduced at the indicated time points compared to the other two control groups. All the results were presented as the mean ± standard deviation (S.D.) based on ≥ 3 replicates involving ≥ 3 samples. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus control groups.

    Journal: EBioMedicine

    Article Title: LncRNA PICSAR promotes cell proliferation, migration and invasion of fibroblast-like synoviocytes by sponging miRNA-4701-5p in rheumatoid arthritis

    doi: 10.1016/j.ebiom.2019.11.024

    Figure Lengend Snippet: Verification of lncRNAs and effect of lncRNA PICSAR suppression on the proliferation of rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs). (a) Quantitative real-time reverse transcription PCR (qRT-PCR) verified 5 selected lncRNAs with the highest up-regulated differential expression according to the microarray analysis. LncRNA PICSAR is the most significantly up-regulated in RA-FLSs. (b) LncRNA PICSAR was also over-expressed in the synovial fluid of RA patients compared with the control group by qPCR. (c) The inhibition efficiency of specific PICSAR small interference RNA (siRNA) in RA-FLSs was detected by qRT-PCR. (d) Compared with negative control, the expression of p-ERK1/2 in the total ERK1/2 protein was significantly decreased in the RA-FLSs after transfection. (e) PICSAR knockdown in RA-FLSs decreases cell proliferation assessed by a Cell Counting Kit-8 assay. The absorbance at 450 nm wavelength of PICSAR-siRNA group were significantly reduced at the indicated time points compared to the other two control groups. All the results were presented as the mean ± standard deviation (S.D.) based on ≥ 3 replicates involving ≥ 3 samples. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus control groups.

    Article Snippet: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology) with minor modifications.

    Techniques: Quantitative RT-PCR, Expressing, Microarray, Inhibition, Negative Control, Transfection, Cell Counting, Standard Deviation