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All (3)
MilliporeSigma
Cell Signaling
Santa Cruz
 

About 8 products / 11 snippets / 6 images

80 / 100
Bioz Stars
Rabbit-anti-PS1 C-terminus (CT)
Cell Signaling Technology  
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Techniques
 
Article Snippets
"The membranes were probed with the following antibodies overnight at 4 °C on a shaker: mouse anti-Syt-1 (Millipore) or rabbit-anti-PS1 C-terminus (CT) (Cell Signaling)."  (see more)
Missing: terminal fragment ctf 
80 / 100
Bioz Stars
Rabbit anti-PS1 C-terminus (CT)
Cell Signaling Technology  
star_border
Techniques
 
Article Snippets
".. with either rabbit anti-GFP (Abcam, Cambridge, MA) as a control or rabbit anti-PS1 C-terminus (CT) (Cell Signaling, Danvers MA) antibodies on a rotator overnight at 4 °C. .."  (see more)
Missing: terminal fragment ctf 
2+  more images
The β-secretase BACE1 interacts with γ-secretase at endogenous levels in WT mouse brain microsomal lysate. (A) 1% CHAPSO-solubilized lysates of WT mouse brain microsomes were immunoprecipitated for BACE1 or ITGα1, ITGα2, and ITGβ1 as controls. IPs were blotted to probe for coIP of the γ-components NCT and <t>PS1-NTF.</t> (B) 1% CHAPSO-solubilized lysates of WT mouse brain microsomes were loaded onto BN-PAGE followed by second-dimension SDS-PAGE and blotted with antibodies to the indicated proteins. *, nonspecific signal; m and im, mature and immature forms.
IP
SDS-PAGE
Effect of EA on changes in mRNA expressions of Notch signaling pathway-related factors Notch1 (A), Notch3 (B), Notch4 (C), <t>PS1</t> (D) and Hes1 (E; real-time qPCR) in injured spinal cord of rats. The mRNA expression level was calculated using the 2 -∆∆Ct analytical method. * P < 0.05, ** P < 0.01, *** P < 0.001, vs . control group. Data are expressed as the mean ± SD ( n = 3; one-way analysis of variance and Tukey's post hoc test). The experiment was repeated in triplicate. SCI: Spinal cord injury; EA: electroacupuncture; qPCR: quantitative polymerase chain reaction.
EXP
CON
qPCR
De novo Aβ generation in HMW FPLC fractions of microsomes from HEK293-swAPP cells. (A) FPLC fractions (Superose 6 Increase column) of 1% CHAPSO-solubilized microsomes of HEK293/swAPP cells were blotted with antibodies to the indicated proteins. (B) Unfractionated microsomes were loaded onto 2D BN/SDS-PAGE and blotted for <t>PS1-NTF.</t> (C) FPLC fractions were incubated at 37°C for 12 h. Aβx-40 and x-42 de novo generation was quantified by ELISA from each fraction after concentration; left y-axis, Aβx-40; right y-axis, Aβx-42 ( n = 3; mean ± SD). Below: A relative ratio of Aβx-42/x-40 was calculated; the purple line represents 0.2. (D) FPLC fractions were incubated at 37°C for 12 h with L685,458 (5 µM) and with DMSO as the control. Aβx-40/x-42 de novo generation was measured by ELISA on each fraction after concentration ( n = 2; means ± SD). (E) As in D, fractions #1–24 were incubated at 37°C for 12 h with 10 µM JNJ-40418677 or DMSO as the control. Aβx-37/x-40/x-42 de novo generation were measured by ELISA from each fraction ( n = 2; means ± SD; unpaired Student’s t test: *, P < 0.05; **, P < 0.01; ***, P < 0.001). (F) 1% CHAPSO-solubilized microsomes from HEK293/swAPP pretreated with 2.5 µM AZD3293 for 24 h (DMSO as the control) were fractionated on a Superose 6 Increase column, and fractions were incubated at 37°C for 12 h. Aβx-40 de novo generation was measured by ELISA from each fraction ( n = 3; means ± SD); unpaired Student’s t test: **, P < 0.01; ****, P < 0.0001. (G) Holo-APP and PS1-NTF immunoblots of FPLC fractions before their 37°C de novo incubation from cells treated with 2.5 µM AZD3293 or DMSO for 24 h (m, mature; im, immature; DARK, longer exposure).
SDS-PAGE
INCU
ELISA
CO-A
WB
Effect of EA on changes in protein expressions of Notch signaling pathway-related factors Notch1, Notch3, Notch4, <t>PS1</t> and Hes1. (A) Western blotting bands for Notch1, Notch3, Notch4, PS1 and Hes1 in different groups. (B) Quantification of protein expression of Notch1, Notch3, Notch4, PS1 and Hes1. Y -axis indicates the relative optical density of protein bands (the ratio of target grayscale values to loading control grayscale values). * P < 0.05, ** P < 0.01, *** P < 0.001, vs . control group. Data are expressed as the mean ± SD ( n = 3; one-way analysis of variance and Tukey's post hoc test). The experiment was repeated in triplicate. SCI: Spinal cord injury; EA: electroacupuncture; d: day(s).
WB
EXP
CON
×
80 / 100
Bioz Stars
Anti PS1 C-terminal domain
Santa Cruz  
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Techniques
 
Article Snippets
"The resultant pellet was subjected to SDS-PAGE and Western blotting by goat polyclonal antibody against the PS1 C-terminal domain (1:250) (Santa Cruz Biotechnology)."  (see more)
Missing: fragment ctf 
85 / 100
Bioz Stars
Anti Anti PS1-C
Santa Cruz  
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Techniques
 
Article Snippets
".. RRID: AB_2170581; Santa-Cruz); for PS1-C, EP-2000Y (rabbit, RRID: AB_1310605; Abcam); for PS2-C, EP-1515Y (rabbit, RRID: AB_882202; Abcam); for NCT, SC-14369 (goat, RRID: AB_2282503; Santa-Cruz); for Aph-1aL, PRB-550P (rabbit, .."  (see more)
IP
SDS-PAGE
Missing: terminal fragment 
85 / 100
Bioz Stars
Mouse anti-PS1 C-terminal fragment (PS1CTF)
MilliporeSigma  
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Techniques
 
Article Snippets
".. Billerica, MA, USA), mouse anti-Nct (612290; BD Biosciences, Stockholm, Sweden), mouse anti-PS1 C-terminal fragment (PS1CTF) (MAB5232; Millipore), rabbit anti-PS1-NTF (529591; Calbiochem, San Diego, CA, USA), rabbit anti-SLC2A13 .."  (see more)
80 / 100
Bioz Stars
Anti Goat polyclonal IgG PS1 (C-20)
Santa Cruz  
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Techniques
 
Article Snippets
".. Biotechnology); goat polyclonal IgG Notch4 (L-16) (1:1,000; Santa Cruz Biotechnology); goat polyclonal IgG PS1 (C-20) (1:1,000; Santa Cruz Biotechnology); and rabbit polyclonal IgG Hes1 (H-140) (1,000; Santa Cruz Biotechnology). .."  (see more)
EXP
CON
qPCR
Missing: terminal fragment ctf 
80 / 100
Bioz Stars
Mouse monoclonal anti-PS1C-terminal-fragment
MilliporeSigma  
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Techniques
 
Article Snippets
".. IL, USA); rabbit polyclonal antiAPP-C-terminal (1:6,000: Calbiochem, Nottingham, UK); mouse monoclonal anti-PS1C-terminal-fragment (CTF, 1:1,000: Millipore, Chemicon international, Germany) or mouse monoclonal anti--tubulin (1:5,000: Sigma). .."  (see more)
80 / 100
Bioz Stars
Anti PS1 C-terminus
MilliporeSigma / Sigma Aldrich  
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Techniques
 
Article Snippets
"Abstract: The 37–43 amino acid Ab peptide is the principal component of b-amyloid deposits in Alzheimer’s disease (AD) brain, and is derived by serial proteolysis of the amyloid precursor protein (APP) by band c-secretase. c-Secretase also cl ..."  (see more)
Missing: terminal fragment ctf