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All (17)
Takara Bio
QIAGEN
Toyobo
Thermo Fisher
Yeasen Biotech
Bioteke
MilliporeSigma
Beijing CWBio
9 More...
 

About 40 products / 503 snippets / 4 images

99 / 100
Bioz Stars
PrimeScript RT Master Mix
Takara Bio  
Catalog#: RR036A
Techniques
Real-time Polymerase Chain Reaction, Expressing, Isolation (see more)
Article Snippets
".. RNA was extracted from whole blood of the PCD patients and reverse-transcribed using the RR036A kit (TAKARA, Japan)."  (see more)
99 / 100
Bioz Stars
PrimeScript RT Master Mix
Takara Bio  
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Catalog#: RR036A
Techniques
Real-time Polymerase Chain Reaction, Expressing, Isolation (see more)
Article Snippets
".. RNA was extracted from whole blood of the PCD patients and reverse-transcribed using the RR036A kit (TAKARA, Japan)."  (see more)
99 / 100
Bioz Stars
PrimeScript RT Reagent Kit with gDNA Eraser
Takara Bio  
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Catalog#: RR047A
Techniques
Real-time Polymerase Chain Reaction, Expressing, Isolation, Synthesized (see more)
Article Snippets
"The cDNA was then synthesized using the Primescript RT Master Mix Kit (Takara, Cat No. RR047A), and qPCR was carried out with the use of TB Green® Premix .."  (see more)
( A ) The correlation between FTO and <t>CTNNB1</t> <t>RNA</t> expression levels in the TCGA dataset for HNSCC assessed by using the GEPIA online tool ( http://gepia.cancer-pku.cn/ ). ( B ) m 6 A dot blot assay of knockdown and replenishment of wild-type (WT) and mutant (MT) FTO in FaDu cells. Methylene blue staining was used as the control. ( C ) RT-qPCR arrays were used to detect the RNA expression levels of CTNNB1 after FTO knockdown in FaDu cell, normalized to GAPDH. Data are presented as the mean ± SD; **P < 0.01. ( D ) CTNNB1 RNA expression levels were analyzed in FTO-WT and FTO-mutant FaDu cells, normalized to GAPDH. Immunoblot assay of CTNNB1 protein levels in FaDu cells upon FTO knockdown and FTO-WT and FTO-mutant. Data are presented as the mean ± SD, **P < 0.01. ( E ) m6A RIP and <t>qRT-PCR</t> analysis of m 6 A levels in CTNNB1 mRNA in shFTO-1/2 or corresponding wild-type cells. Data are expressed as the mean ± SD; *P < 0.05.
RNA-EXP
DOT-BLOT
MUTA
STAIN
qRT-PCR
(A-B) Human glioblastoma cells U251 were transfected with empty control or plasmid to overexpress MeCP2, and subjected to either western blotting or <t>qRT-PCR</t> to detect ADAM10 protein (A) or mRNA (B) . N≥5. (C-D) U251 cells were transfected with MeCP2 and a miR-197 inhibitor or scramble control, and subjected to qRT-PCR for ADAM10 mRNA (C) or Western blotting for ADAM10 protein (D) . N≥3. GAPDH was used as loading control in both experiments. (E) A 7mer-m8 miR-197 binding site predicted by TargetscanHuman. The alignment of miR-197 to human and mouse ADAM10 3’-UTR and the point mutation G>A in A10-I-Mut are illustrated. (F) A miR-197 binding site predicted by RNAhybrid is highly conserved between human and mouse. The deletion mutation in A10-II-Mut was also illustrated. (G) Luciferase reporter plasmids for ADAM10 3’-UTR were transfected into U251 cells with miR-197 mimics or control. N≥8. (H) Biotin-labeled miR-197 and control scramble were transfected into U251 cells and the ADAM10 3’-UTR pulled down by miR-197 or control scramble were quantified by qRT-PCR. N=4. (I-L) U251 cells were transfected with either control empty vector or MeCP2 expressing plasmids and cultured for 24hrs. RNA from these cells were extracted and subjected for qRT-PCR. N=9. The levels of pri-miR-197, pre-miR-197, mature miR-197, and pre-miR-134 were shown in (I-L) , respectively. All statistical data are represented as means ± SEM. * p <0.05, ** p <0.01, *** p <0.001. (M) A scheme illustration for MeCP2 regulation on miR-197 and ADAM10.
TNSF
PLASMID-P
WB
qRT-PCR
BIND-A
<t>CCNK</t> <t>mRNA</t> Level in Lymphocytes of Affected Subjects and the Morpholino Knockdown Approach of Zebrafish Ccnk
U251 cells transfected with MeCP2 expressing plasmids were also subjected to RNA-IP with MeCP2 antibody or control IgG. The levels of pri-miR-197 and pri-miR-134 co-precipitated with MeCP2 were quantified by <t>qRT-PCR.</t> N≥5, all statistical data represent means ± SEM. *** p <0.001.
TNSF
EXP
qRT-PCR
×
98 / 100
Bioz Stars
PrimeScript IV 1st strand cDNA Synthesis Mix
Takara Bio  
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Catalog#: 6215A
Techniques
Real-time Polymerase Chain Reaction, Expressing, Synthesized, Isolation (see more)
Article Snippets
".. Kit (DP430, TIANGEN, Beijing, China). the total RNA was reverse-transcribed using 162 the PrimeScrip RT Kit with gDNA Eraser (6215A, Takara, Kyoto, Japan). then qRT-163 PCR assays were performed using the PowerUp™ SYBR™ Green .."  (see more)
98 / 100
Bioz Stars
One-Step TB Green PrimeScript RT-PCR Kit II
Takara Bio  
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Catalog#: RR086A
Techniques
Reverse Transcription Polymerase Chain Reaction (see more)
Article Snippets
".. using SYBR Green PCR Master Mix in triplicate format for each gene utilizing Takara One-Step SYBR®PrimeScript™ RT-PCR Kit II (RR086A, Japan) based on the manufacturer's protocol by Applied Biosystems 7500 System. .."  (see more)
99 / 100
Bioz Stars
PrimeScript RT Reagent Kit
Takara Bio  
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Catalog#: RR037A
Techniques
Real-time Polymerase Chain Reaction, Expressing, Isolation, Synthesized (see more)
Article Snippets
"Synthetic complementary DNA (cDNA) was generated by mixing total RNA with a Takara PrimeScript RT reagent kit."  (see more)
Missing: master 
98 / 100
Bioz Stars
EmeraldAmp GT PCR Master Mix
Takara Bio  
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Catalog#: RR310A
Techniques
Polymerase Chain Reaction, Amplification, Sequencing (see more)
Article Snippets
".. Green Cytotoxicity Assay Promega Cat#G8742 Dual-Luciferase Reporter Assay System Promega Cat#E1910 MiniBEST Universal RNA Extraction Kit TAKARA Cat#9767A PrimeScript 1st strand cDNA Synthesis Kit TAKARA Cat#6110A EmeraldAmp GT .."  (see more)
99 / 100
Bioz Stars
Premix Ex Taq
Takara Bio  
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Catalog#: RR390A
Techniques
Real-time Polymerase Chain Reaction, Expressing, Quantitative RT-PCR (see more)
Article Snippets
".. kit instructions (Takara, RR036A); 559 gene expression was detected through SYBR Premix Ex Taq kit operation steps (Takara, RR420)."  (see more)
96 / 100
Bioz Stars
QIAGEN Multiplex PCR Kit
QIAGEN  
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Catalog#: 206145
Techniques
Polymerase Chain Reaction, Multiplex Assay, Amplification, Sequencing (see more)
Article Snippets
"Many commercial master mixes seem to function well in the detection of SARS-CoV-2, although a detailed side-by-side comparison of the numerous commercial reagents is lacking. compared four popular one-step RT-PCR kits (Takara One Step PrimeScript III kit, Qiagen .."  (see more)
96 / 100
Bioz Stars
One Step PrimeScript III RT-PCR Kit
Takara Bio  
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Catalog#: RR600A
Techniques
Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction (see more)
Article Snippets
"In the N region, the kit used for detection is One Step Prime Script III RT-qPCR Mix (TAKARA BIO, Japan), Primer/Probe Set (TAKARA BIO, Japan), and Positive Control RNA Mix (2019-nCov) .."  (see more)
Missing: master 
99 / 100
Bioz Stars
PrimeScript Reverse Transcriptase
Takara Bio  
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Catalog#: 2680A
Techniques
Real-time Polymerase Chain Reaction, Synthesized, Expressing (see more)
Article Snippets
".. isolated from LECs using EZ-press RNA puri cation kit. cDNA synthesis using Prime Script reverse transcriptase Master Mix (TaKaRa) according to the manufacturer's instruction."  (see more)
99 / 100
Bioz Stars
PrimeScript RT-PCR Kit
Takara Bio  
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Catalog#: RR014A
Techniques
Reverse Transcription Polymerase Chain Reaction (see more)
Article Snippets
"Total RNA from various tissues was extracted using the MiniBEST Plant RNA Extraction Kit (TaKaRa) and reverse-transcribed using PrimeScript RT (TaKaRa)."  (see more)
96 / 100
Bioz Stars
One Step PrimeScript III RT-qPCR Mix, with UNG
Takara Bio  
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Catalog#: RR601B
Techniques
Quantitative RT-PCR, Real-time Polymerase Chain Reaction (see more)
Article Snippets
".. a single closed tube using a one step RT-PCR kit (One Step PrimeScript III RT-qPCR Mix, with UNG; Takara Bio Inc., Kusatsu, Japan), according to the manufacturer’s instructions."  (see more)
Missing: master 
93 / 100
Bioz Stars
PrimeScript™ RT reagent Kit
Thermo Fisher Scientific / Life Technologies  
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Techniques
 
Article Snippets
"Abstract: Marek’s disease virus type 1 (MDV-1) is a representative oncogenic Alpha herpesvirus that causes an immunosuppressive and neoplastic lymphoproliferative avian disease, namely Marek’s disease (MD).. The rapidonset T-cell lymphoma in ..."  (see more)
Missing: master