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All (40)
Cell Signaling
MilliporeSigma
Santa Cruz
Upstate Biotech
AssayQuant Technologies
PamGene
Merck KGaA
AnaSpec
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About 182 products / 347 snippets / 56 images

94 / 100
Bioz vStars
H1-7 (histone H1 phosphorylation site), PKA Substrate
Aladdin Scientific  
Catalog#: H332884-1MG
Description
"H1-7 (histone H1 phosphorylation site), PKA Substrate is a synthetic peptide that acts as a pseudo substrate for PKA (cAMP-dependent protein kinase). This peptide was derived from the calf thymus histone H1 amino acid sequence"  (see more)
80 / 100
Bioz Stars
Phosphorylated substrates
Thermo Fisher Scientific / Molecular Probes  
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Techniques
 
Article Snippets
"For MEKK1, JNK1, and p38, samples were resolved by SDS/PAGE, and phosphorylated substrates were detected and quantified by using a PhosphorImager (Molecular Probes)."  (see more)
80 / 100
Bioz Stars
Phosphorylation substrate
Cell Signaling Technology  
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Techniques
 
Article Snippets
".. ImmunoResearch). p38 assays used GST-ATF-2 as a phosphorylation substrate ( 40 ) (Cell Signaling Technology)."  (see more)
52+  more images
Phosphorylation of <t>PKA</t> <t>substrates</t> in the NAc of sensitized rats is decreased in early withdrawal but increased in late withdrawal. (a-left panel) Immunoprobing NAc cross-linked tissue (20 or 10 μg of total protein loaded onto the gel) with the PKA substrate antibody in the presence of peptide vehicle (PKA + Veh.) results in the detection of nine immunoreactive bands. (a-center panel) Immunoreactivity is unchanged by pre-incubation of the PKA substrate antibody with the PKC substrate peptide (PKA + PKC pep.). (a-right panel) Immunoreactivity is eliminated by preincubation with the PKA substrate peptide (PKA + PKA pep.). (b) Representative immunoblot demonstrating the bands scanned to assess overall PKA phosphorylation (OVERALL, left lane) and individual PKA substrates (right lane). (c–e) Overall, PKA substrate phosphorylation is decreased on WD1, unchanged on WD7, and increased on WD21 in sensitized rats. WD 1: SAL (n = 12), SENS (n = 7), NON (n = 9); WD 7: SAL (n = 16), SENS (n = 12), NON (n = 16); WD 21: SAL (n = 15), SENS (n = 10), NON (n = 12). Data (mean+S.E.M.) are normalized to SAL controls. *p < 0.05, **p < 0.01, significantly different from SAL group.
Phospho-proteomics
INCU
WB
Effect of transfection and RNAi on <t>GSK3β</t> expression is shown. (A) Cell GSK3β content after transfection with empty vector (EV), constitutively active (S9A), or WT GSK3β (top); β-actin loading control (bottom). (B) Total GSK3β content in nontransfected cells (Nl), in cells transfected with vector only (V-Ctl), a mixture of three (Mix; top), single specific RNAi directed against GSK3β (I, II, or III) or a mixture of nonspecific RNAis (Mix-Non-Specific-RNAi); β-actin is loading control (bottom). Each lane contains 40 μg of total protein; results are representative of at least three separate studies.
TNSF
EXP
PLASMID-P
CON
Alanine mutations of highly conserved Thr-7 and Thr-8 on the N-terminus of <t>GSK3β</t> impairs Ser-9 phosphorylation in vivo and GSK3β autoinhibition by insulin. (A) In the N-terminus of human GSK3β, highly conserved Thr-7 and Thr-8 residues are located adjacent to the inhibitory phosphorylation site Ser-9 within the AKT substrate consensus sequence R-P-R-T7-T8-S9-F. (B) HA-GSK3β wild type (WT) and T-to-A mutant (T7A, T8A, S9A) proteins were expressed in HEK293T cells, purified by anti-HA agarose beads, and incubated in vitro with cold ATP and His-Akt1 at 30°C for various time points as indicated. Total and phospho-S9 specific signals of HA-GSK3β were detected on Western blot. Phospho-S9 signals were quantified and normalized against total GSK3β signals. (C) HA-GSK3β (WT, T7A, T8A) proteins were expressed in HEK293T cells under normal fed condition. Whole cell extracts were Western blotted for total and phospho-S9 signals of endogenous and HA-tagged GSK3β. Shown are data of duplicates. (D) HA-GSK3β (WT, T7A) proteins were expressed in HEK293T cells under normal fed condition. After anti-HA immunoprecipitation, total and phospho-S9 signals of HA-GSK3β as well as endogenous AKT co-immunoprecipitated with HA-GSK3β were analyzed by Western blot. Shown are data of triplicates. (E) HA-GSK3β WT or mutant (T7A, T8A) proteins were expressed in HEK293T cells. After overnight starvation in 0.1% low-serum medium, cells were treated with 100 nM insulin for various time ponits as indicated. Phospho-S9 specific and total GSK3β signals in whole cell extracts (WCE) or in anti-HA immunoprecipitates were analyzed by Western blot. Shown are representative data from three independent experiments. (F) HA-GSK3β WT and mutant (T7A, T8A, S9A) proteins were expressed in HEK293T cells. After starvation and insulin stimulation (100 nM, 60 min), cells were lysed and HA-GSK3β proteins were affinity-purified on anti-HA agarose beads. HA-GSK3β WT proteins purified from unstimulated cells served as the control. In vitro kinase assay was conducted to assess GSK3β activity toward p-GS peptide substrates. Shown are GSK3β activities relative to the control, mean ± SD, n=3 independent experiments, *P<0.01.
INVIVO
SEQ
MUTA
PURI
INCU
The CB 1 receptor protects cultured striatal cells from NMDA-induced excitoxicity via PI3K/Akt/mTORC1/BDNF. ( a ) STHdh Q7/Q7 cells were incubated for the times indicated with vehicle, 0.5- μ M THC or 10-nM HU-210. Cells were lysed and western blot analyses were conducted. Quantification of mean optical density (O.D.) values relative to those of loading controls (respective total proteins, or α -tubulin in the case of <t>PKA</t> <t>phosphorylated</t> <t>substrates)</t> as well as representative blots are shown ( n =3–4 experiments). ( b , c ) STHdh Q7/Q7 cells were preincubated for 5 h in Locke's solution with or without 1-mM NMDA together with vehicle, 0.5- μ M THC, 10-nM HU-210, 0.2- μ M wortmanin, 0.1- μ M Akti-1/2, 30-nM rapamycin and/or 25-nM K252a, and subsequently incubated for 24 h in NMDA-free medium. Relative cell viability is shown ( n =6–8 experiments). ( d ) STHdh Q7/Q7 cells were transfected with a non-targeted siRNA or with siRNAs directed against BDNF or TrkB , and subsequently incubated for 5 h with or without NMDA, THC and/or HU-210 as in b . Relative cell viability is shown ( n =4–6 experiments). ( e ) Primary mouse striatal neurons were incubated for 30 min in Locke's medium with or without 50- μ M NMDA, together with vehicle, 0.3- μ M THC, 10-nM HU-210, 0.25- μ M SR141716, 0.1- μ M Akti-1/2, 20-nM rapamycin and/or 10-nM K252a, and subsequently incubated for 2 h in NMDA-free medium. Relative cell viability is shown ( n =4–6 experiments). Data were analyzed using ANOVA with post hoc Student-Newman-Keuls test. * P <0.05 and ** P <0.01 from the corresponding vehicle-treated cells
CELL-CTR
INCU
WB
TNSF
×
80 / 100
Bioz Stars
Substrate phosphorylation
Selleck Chemicals  
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Techniques
 
Article Snippets
".. and ipatasertib, which binds to AKT in an ATP-competitive manner and prevents substrate phosphorylation [ ] (Selleck Biotech Ltd., Tokyo, Japan)."  (see more)
80 / 100
Bioz Stars
Anti Phosphorylated substrates
The Antibody  
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Techniques
 
Article Snippets
".. kit can further comprise an antibody or affinity product that recognizes phosphorylated substrates, wherein the antibody or affinity product is directly labeled with an element tag. .."  (see more)
92 / 100
Bioz Stars
Phosphorylated Akt substrates
Cell Signaling Technology  
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Techniques
 
Article Snippets
"A second antibody that is more specific for phosphorylated Akt substrates (catalog no. 9614; also from Cell Signaling Technology) also detected wild-type phosphorylated RdRp but not the mutated form .."  (see more)
86 / 100
Bioz Stars
Phosphorylated PKA substrates
Cell Signaling Technology  
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Techniques
 
Article Snippets
".. antibody (clone 9E10; Abcam,Cambridge,MA) orwith an antibody directed against phosphorylated PKA substrates (Cell Signaling Technology, Beverly, MA)."  (see more)
91 / 100
Bioz Stars
Phosphorylated PKA substrates
Cell Signaling Technology  
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Catalog#: 5565
Techniques
Incubation, Immunofluorescence, Staining, Software, Microscopy (see more)
Article Snippets
".. onto a polyvinylidene fluoride membrane for immunoblotting with antibody recognizing phosphorylated PKA substrates (Cell Signaling Technology; see previous section)."  (see more)
85 / 100
Bioz Stars
Phosphorylated Akt substrate
Cell Signaling Technology  
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Techniques
 
Article Snippets
"Polyclonal anti-P-Akt(308), P-Akt(473), and phosphorylated Akt substrate were obtained from Cell Signaling Technology (Danvers, MA)."  (see more)
88 / 100
Bioz Stars
Phosphorylated substrate peptide
GL Biochem  
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Techniques
 
Article Snippets
"Substrate peptide for PKA (TAMRA-LRRASLG, 98%), and phosphorylated substrate peptide were purchased from GL Biochem (Shanghai, China)."  (see more)
85 / 100
Bioz Stars
Phosphorylated PKA substrates
Upstate Biotech  
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Techniques
 
Article Snippets
"Levels of PKA-phosphorylated HSPDE3B in cells were detected using an antiserum directed at phosphorylated PKA substrates (Upstate Biotechnology, Inc.)."  (see more)
92 / 100
Bioz Stars
Phosphorylated PKA substrate
Cell Signaling Technology  
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Techniques
 
Article Snippets
".. The NC membrane was subsequently incubated with an antibody against phosphorylated PKA substrate (Cell Signalling Technology, USA, 9624) and phosphorylated tyrosine (Cell Signalling Technology, USA, 4G10) diluted in .."  (see more)
85 / 100
Bioz Stars
Phosphorylated PKA substrate
Cell Signaling Technology  
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Techniques
 
Article Snippets
".. MA) (45), phospho- and total phospholamban (Abcam, Cambridge, MA) (46, 47), phosphorylated PKA substrate (Cell Signaling Technology, Beverly, MA) (48), and the loading control Ran (Santa Cruz Biotechnology, .."  (see more)
94 / 100
Bioz vStars
H1-7 (histone H1 phosphorylation site), PKA Substrate
Aladdin Scientific  
star_border
Catalog#: H332884-1MG
Description
"H1-7 (histone H1 phosphorylation site), PKA Substrate is a synthetic peptide that acts as a pseudo substrate for PKA (cAMP-dependent protein kinase). This peptide was derived from the calf thymus histone H1 amino acid sequence"  (see more)