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All (17)
Miltenyi Biotec
Becton Dickinson
Immunostep
RD Biotech
Bio-Rad
Abnova
Exbio Praha
SONY
9 More...
 

About 49 products / 59 snippets / 14 images

94 / 100
Bioz vStars
CD9 Mouse mAb (APC)
Aladdin Scientific  
Catalog#: AB180145-100T
Description
"Application:Flow: 5μL/testFor flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood."
93 / 100
Bioz vStars
CD9 Polyclonal Antibody, APC-Cy5.5 Conjugated
Bioss  
Catalog#: BS-23035R-APC-CY5-5
Description
"CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a"  (see more)
91 / 100
Bioz Stars
CD9 APC
Becton Dickinson / BD Biosciences  
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Techniques
 
Article Snippets
".. Cambridge, United Kingdom), 5 μL of CD63 PE-Cy7 (BD Biosciences), and 5 μL of CD9 APC (BD Biosciences), or matching isotype controls (FITC, PE, and APC obtained from Life .."  (see more)
EXP
MARK
CON
80 / 100
Bioz Stars
Anti CD9-APC
Becton Dickinson / BD Biosciences  
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Techniques
 
Article Snippets
".. CD34-Pacific Blue (BD Bioscience, 1:50), Sca1-V500 (eBioscience, 1:500), CD26-PE-Cy7 (Biolegend, 1:500), CD9-APC (BD Bioscience, 1:100), and CD9-FITC (eBioscience, 1:100)."  (see more)
10+  more images
Exosome markers were assessed by ( a ) exoELISA and ( b ) FACS. The expression of the exosome marker ( a ) CD81 was not different (20.94 ± 4.84 × 10 6 exosomes/µL versus 39.36 ± 3.14 × 10 6 exosomes/µL; p = n.s.) compared to isotype control. The expression ( b ) of exosomes positive for <t>CD9</t> (74 ± 4% versus 75 ± 2%; p = n.s), HSP70 (22 ± 8% versus 23 ± 8%; p = n.s) or CD81 (11 ± 3% versus 12 ± 4%; p = n.s.) and CD63+ exosomes (14 ± 1% versus 17 ± 2; p = n.s.) was unchanged upon anti-CD3 antibody treatment compared to isotype control.
EXP
MARK
CON
Characterization of Exoquick-precipitated plasma exosomes. ( A–C ) Atomic Force Microscopy (AFM). ( A) Representative AFM micrograph (2.5 μm × 2.5 μm) of mBC exosomes enriched by ExoQuick, diluted in PBS and deposited on a mica substrate. (B) Height profile of a single vesicle (blue line in panel A) matching the typical lateral dimensions of exosomes (<150 nm). (C) Quantification of the exosome diameter: histogram show the distribution of the diameter of exosomes, as extracted by grain analysis (Gwyddion) of three distinct AFM images of the same sample. (D) Nanoparticle Tracking Analysis (NTA). Representative histogram showing the particle size distribution of an exosome preparation analyzed by Nanosight and diluted 1:1000 in water. (E–G) Western Blot. ( E) After ExoQuick precipitation, samples were subjected to floatation on a linear sucrose gradient. Protein distribution in the gradient is shown as µg of proteins recovered in each fraction (grey line; scale on the right). Enlarged data (black line; scale on the left) are also shown. (F) Ponceau staining of the gel. (G) Cropped immunoblot showing <t>CD9</t> immunoreactivity of the different recovered fractions (full length blot is displayed in Supplementary Fig. ). (H) Scanning Electron Microscopy (SEM). Representative pictures, acquired at different magnification, of a sample whose CD9 positive fractions (fractions 3–5) were pooled and subjected to SEM analysis. (I) Dynamic light scattering (DLS) analysis. CD9 positive fractions 3–5 were pooled and analyzed by DLS. Histograms represent the size distribution of the vesicles. The profile shows the presence of distinct populations. The first one with an average size of about 30 nm and the second one of 110 nm. A population with an average diameter of 450 nm was also observable, probably due to protein aggregation induced by ExoQuick treatment.
Clinical Proteomics
MICRO
WB
STAIN
EM
(A) WB of whole cell lysate (WCL, 10 μg) from a stable OLN-93 cell line expressing human CD63-EGFP and EVs (10, 20 μg) from 100,000 x g pellet. <t>CD9,</t> CD63, Tsg101, core histones (H2A, H2B, H3 and H4), Golgi marker GM130 as well as Actb are identified using specific antibodies. Approximate molecular mass is shown in kDa. (B-E) Immuno-TEM micrographs of OLN-93 EVs 100,000 x g .using primary antibodies as indicated and secondary antibodies conjugated to with 10 nm gold particles. (B) Anti-CD63. (C) Anti-histone H1 detected using protein A immuno-gold (10 nm) to detect the primary antibody. Scale bar in C = 200 nm. (D) Representative immuno-TEM micrographs of OLN-93 cell sections showing anti-CD63 distribution. Insert panel shows a magnified region containing an MVB. The column chart on the right shows quantification of the number of MVBs and ILVs. T otal number of MVBs versus CD63 + MVBs, and total number of ILVs versus CD63 + ILVs are shown as % of total. (E) OLN-93 sections showing immuno-TEM with anti-H1. Each data point in the column chart shows number of ILVs counted per MVB. Percentages in the column charts refer to % positively labelled ILVs compared to the total (given as 100%). Error bars represent the mean ±SD. (F) WB showing localization of a range of membrane, EV and organelle markers together with linker H1 and core histones in OptiPrep purified EVs 100,000 x g (300 µg) fractions from a 12%-36% (w/v) equilibrium density gradient. One 20 th of each fraction was loaded on the gel. Experiments were repeated 3 times, and one representative image is shown.
EXP
MARK
MEM
PURI
Extracellular vesicles characterization. NOTE. A. Nanoparticle tracking analysis (NTA) after size exclusion chromatography of 500µl of plasma. Nanoparticles were only detected in elution fractions 7 to 12 (only these fractions are displayed on the graph here). NTA provided the number and size of particles detected. The mean size of the particles was 150nm, that corresponds to the expected size of extracellular vesicles (EVs). A’. The particles were further characterized by using EVs inclusion markers <t>(CD9,</t> CD81 and CD63) and exclusion markers measured by DELFIA–ELISA (HAS) or human IgG and ApoB measured by dot blot. Protein content was measured by bicinchoninic acid assay. EVs inclusion markers were highly present in fractions 7 to 12 whereas exclusion markers were almost absent. B. Characterization of the neurally– derived EVs (NDEVs) population by flow cytometry (NovoCyte Quanteon). B.1. Full EVs population scatter plot using CFSE–FITC staining (x–axis) and side scatter measurement (SSC, y–axis). Gating (R1) was performed to isolate EVs (FITC–positive). B.2. Double staining was performed using CFSE– FITC and <t>CD9–APC,</t> as validation step for the EVs population scattering. 99% of the events present in the R1 gate (positive for CFSE–FITC) were positive for CD9–APC, which confirms that the events detected in R1 are EVs. B.3. Triple staining for NDEVs population detection was performed using CFSE–FITC, CD9–APC and CD56–eFluor450. The estimated NDEVs population (R3) represent 3% of the total EVs population (R1).
SEC
ELISA
DOT-BLOT
ACID-A
DER-A
×
90 / 100
Bioz Stars
Anti CD9‐APC
Becton Dickinson / BD Biosciences  
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Techniques
 
Article Snippets
".. by supplier: CD73‐PECy7, BD; CD133‐PE, Miltenyi; CD29‐BV510, BD; SSEA‐1APC, BD; CD90‐APC, BD; CD9‐APC, BD; CD200‐V450; CD49f‐BV650; EGFR1‐PE, BD; GD2‐PE, BD; CD184‐PE, BD; SSEA‐4‐PerCP‐Cy5.5."  (see more)
80 / 100
Bioz Stars
CD9-APC
Miltenyi Biotec  
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Techniques
 
Article Snippets
"Isolated EVs were stained with CD9-APC (Miltenyi Biotec GmbH, Bergisch Gladbach, D), CD63-PE-Cy7 (BD, New Jersey, USA) and CD83-PE (BD, New Jersey, USA) .."  (see more)
80 / 100
Bioz Stars
Anti CD9-APC
Miltenyi Biotec  
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Techniques
 
Article Snippets
"The following antibodies were used: CD9-APC (Miltenyi Biotech, clone SN4), CD63-APC (Miltenyi Biotec, clone H5C6), and CD81-APC (Beckman Coulter, clone JS64)."  (see more)
91 / 100
Bioz Stars
Anti Anti CD9‐APC
Miltenyi Biotec  
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Techniques
 
Article Snippets
".. FACS AriaIII) as recommended by supplier: CD73‐PECy7, BD; CD133‐PE, Miltenyi; CD29‐BV510, BD; SSEA‐1APC, BD; CD90‐APC, BD; CD9‐APC, BD; CD200‐V450; CD49f‐BV650; EGFR1‐PE, BD; GD2‐PE, BD; CD184‐PE, BD; .."  (see more)
80 / 100
Bioz Stars
CD9-APC-Vio770
Miltenyi Biotec  
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Techniques
 
Article Snippets
".. BD), GLAST-PE (1:50, Miltenyi) or GLAST-APC (with EdU detection; 1:50, Miltenyi), and CD9-APC-Vio770 (1:20, Miltenyi)."  (see more)
80 / 100
Bioz Stars
Anti CD9-APC
Exbio Praha  
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Techniques
 
Article Snippets
"The following antibodies were used: CD9-PE (Exbio, clone MEM-61), CD9-APC (Exbio, clone MEM-61), CD63-PE (Exbio, clone MEM-259), CD81-FITC (BioLegend, clone 5A6), CD81-APC (Beckman Coulter, .."  (see more)
80 / 100
Bioz Stars
Anti CD9 APC
Bio-Rad / AbD Serotec  
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Techniques
 
Article Snippets
".. CD44 APC.Cy7, EpCam FITC, Her2 PE, CD49d APC (BioLegend); CXCR4 FITC, c-MET APC (R&D Systems); CD9 APC (Serotec); CD63 FITC (Santa Cruz Biotechnology)."  (see more)
80 / 100
Bioz Stars
Anti‒CD9-APC
Becton Dickinson / BD Biosciences  
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Techniques
 
Article Snippets
"The pellet was then incubated together with anti‒CD9-APC (M-L13, 341648; BD Biosciences, Franklin Lakes, NJ), anti‒CD63-PE (MEM-259, SAB4700218; Sigma, St. Louis, MO), and anti‒ .."  (see more)
80 / 100
Bioz Stars
Anti-CD9-APC
Becton Dickinson / BD Biosciences  
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Techniques
 
Article Snippets
".. supplemented with 0.5% foetal bovine serum (PBS + 0.5%FBS), and stained using 1× anti-CD9-APC (Cat No 341648; BD, Bioscience), 1× anti-CD63-PE (Cat No 55705; BD Bioscience) and 0.5 μL of BRIC 256 .."  (see more)
80 / 100
Bioz Stars
Anti-CD9-APC
RD Biotech  
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Techniques
 
Article Snippets
".. μl of EVs suspension was incubated overnight at 4 °C with either anti-CD9-APC (R&D Biotech, #FAB1880R-100UG), anti-CD56-eFluor450 (ThermoFisher scientific, #48-0566-42) or both at the .."  (see more)
94 / 100
Bioz vStars
CD9 Mouse mAb (APC)
Aladdin Scientific  
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Catalog#: AB180145-100T
Description
"Application:Flow: 5μL/testFor flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood."
93 / 100
Bioz vStars
CD9 Polyclonal Antibody, APC-Cy5.5 Conjugated
Bioss  
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Catalog#: BS-23035R-APC-CY5-5
Description
"CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a"  (see more)
93 / 100
Bioz vStars
Anti Human CD9 Flow Cytometry Monoclonal Clone HI9A APC Ready To Use
Innovative Research  
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Catalog#: IMSAHUCD9HI9ACAPC100
Description
"Anti Human CD9 Flow Cytometry Monoclonal Clone HI9A APC Ready To Use from Innovative Research is a flow cytometry antibody, buffered in PBS with 0.05% Proclin300, 1% BSA. This flow cytometry antibody has been specifically"  (see more)
93 / 100
Bioz vStars
Mouse CD9 APC-conjugated Antibody
Bio-Techne corporation / R&D Systems  
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Catalog#: FAB5218A
Description
"The Mouse CD9 APC-conjugated Antibody from R&D Systems is a CD9 antibody to CD9. This antibody reacts with Mouse. The CD9 antibody has been validated for the following applications: Flow Cytometry."
93 / 100
Bioz vStars
Anti Human CD9 Flow Cytometry Monoclonal Clone HI9A APC Ready To Use
Innovative Research  
Catalog#: IMSAHUCD9HI9ACAPC100
Description
"Anti Human CD9 Flow Cytometry Monoclonal Clone HI9A APC Ready To Use from Innovative Research is a flow cytometry antibody, buffered in PBS with 0.05% Proclin300, 1% BSA. This flow cytometry antibody has been specifically"  (see more)
93 / 100
Bioz vStars
Mouse CD9 APC-conjugated Antibody
Bio-Techne corporation / R&D Systems  
Catalog#: FAB5218A
Description
"The Mouse CD9 APC-conjugated Antibody from R&D Systems is a CD9 antibody to CD9. This antibody reacts with Mouse. The CD9 antibody has been validated for the following applications: Flow Cytometry."