tpmpa  (Tocris)

Journal: Cell reports

Article Title: Functional maturation of the rod bipolar to AII-amacrine cell ribbon synapse in the mouse retina

doi: 10.1016/j.celrep.2023.113440

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Article Snippet: TPMPA , Tocris , 1040.

Techniques: Recombinant, Knock-Out, Software

Journal: Neuron

Article Title: The sodium-bicarbonate cotransporter Slc4a5 mediates feedback at the first synapse of vision

doi: 10.1016/j.neuron.2024.08.015

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Article Snippet: TPMPA ((1,2,5,6-Tetrahydropyridin-4-yl)methylphosphinic acid) , Tocris Bioscience , #1040; CAS: 182485-36-5.

Techniques: Virus, Recombinant, RNAscope, Multiplex Assay, Gene Expression, Sequencing, Positive Control, Negative Control, Software, Imaging

Journal: bioRxiv

Article Title: Cone bipolar cell synapses generate transient versus sustained signals in parallel ON pathways of the mouse retina

doi: 10.1101/2024.05.13.593825

Figure Lengend Snippet: Presynaptic inhibition does not establish kinetic differences between bipolar cell inputs to ON-T versus ON-S RGCs. (A) Example EM micrograph showing a ribbon dyad (red arrowhead) between a BC and an ON-T RGC and an amacrine cell, with an adjacent feedback inhibitory synapse (yellow asterisk). (B) Bar plots showing the percentage of RGC/AC ribbon dyads with feedback inhibition. Mean ± SEM (n=2 ON-S RGCs, 2 ON-T RGCs) (C) Excitatory currents measured in response to light step increment in control conditions (cyan) and in the presence of GABA receptor antagonists (10 µM SR95531 and 50 µM TPMPA; dashed gray) in an example ON-T RGC. Traces are mean response from six repeats of stimulus presentation. (D) Summary of steady-state amplitudes measured from step responses in control or with GABA receptor antagonists. No significant difference between conditions (p= 0.063; Wilcoxan signed rank test) (E) Control excitatory currents (cyan) or with addition of strychnine (1 µM; dashed gray) to bath in an example ON-T RGC. (F) Summary of strychnine experiments. No significant difference between conditions (p= 0.438; Wilcoxan signed rank test). In (D) and (F), open circles with lines show measurements from individual ON-T RGCs. Filled circles with error bars show mean ± SEM.

Article Snippet: Inhibitory receptor antagonists (SR-95531, TPMPA, strychnine) were obtained from Tocris or Sigma Aldrich and added to the bath solution from concentrated aqueous stock solutions.

Techniques: Inhibition, Control