cppd (InvivoGen)

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InvivoGen cppd
Cppd, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlrl-cppd/CPPD+crystals/us12648953-677-4-7
Average 99 stars, based on 37 article reviews

cppd - by Bioz Stars, 2026-11

99/100 stars

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Recombinant:

Article Title: Piezo1 senses hyphae and initiates host defense against pathogenic fungi.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER anti-CREB1 Proteintech Cat# 12208-1-AP; RRID: AB_2245417 anti-STK38 Proteintech Cat# 55335-1-AP; RRID: AB_2881310 anti-Phospho-CaMK1-α (pThr177) Sigma-Aldrich Cat# SAB4504121 anti-Phospho-CaMKIV Invitrogen Cat# PA5-37504; RRID: AB_2554113 anti-Phospho-NDR1/2 This paper N/A anti-STK38L (OTI4D8) NOVUSBIO Cat# NBP-45983 anti-Mouse CD45 (Clone 30-F11) BioLegend Cat# 103116; RRID: AB_312981 anti-Mouse CD11b (Clone M1/70) BioLegend Cat# 101217; RRID: AB_389305 anti-Mouse Gr-1 (Clone RB6-8C5) BioLegend Cat# 108416; RRID: AB_313381 anti-Mouse F4/80 (Clone BM8) BioLegend Cat# 123126; RRID: AB_893483 anti-Mouse CD11c (Clone N418) BioLegend Cat# 117330; RRID: AB_11219593 anti-Mouse CD3ε (Clone 17A2) BioLegend Cat# 100349: RRID: AB_2565841 anti-Mouse NK1.1 (Clone PK136) BioLegend Cat# 108736; RRID: AB_2563159 Fungal Candida albicans (SC5314) China General Microbial Culture Preservation Management Center CGMCC,2.3629 The Δcph1/Δefg1 mutant Dr. Xinming Jia from Tongji University Grew up in house The Δece1 mutant Dr. Xinming Jia from Tongji University Grew up in house .. Chemicals, peptides, and recombinant proteins LPS Sigma-Aldrich Cat# L2630 Yoda1, purity≥99% (HPLC) TOCRIS Cat# 5586 Nigericin Invivogen Cat# tlrl-nig-5 MSU Crystals Invivogen Cat# tlrl-msu-25 CPPD Crystals Invivogen Cat# tlrl-cppd Nano-SiO2 Invivogen Cat# tlrl-sio CREB inhibitors 666-15 MCE Cat# HY-101120 A pan CaMKs inhibitor KN-62 Selleck Cat# S7422 2 × Phanta Max Master Mix Vazyme Cat# P515-03 Exonuclease III Takara Cat# 2170B DMEM, Powder, High Glucose Gibco Cat# 12100061 100 × L-glutamine Gibco Cat# 25031-81 HEPES Gibco Cat# 15630-80 100 × NEAA Gibco Cat# 11140-50 Recombinant mouse M-CSF (carrier-free,50 ng mL − 1 ) Novoprotein Cat# CB34-500μg Percoll Pharmacia GE Cat# 17-0891-09 RPMI Medium 1640 Gibco Cat# 31800-022 TRIzol reagent Ambion Thermo Fisher scientific Cat# 15596026 (Continued on next page) 20 Cell Reports 44, 115839, June 24, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Taq Pro Universal SYBR qPCR Master Mix Vazyme Biotech Cat# Q712-03 EGTA Sangon Biotech Cat# A600077-0025 Pluronic F-127 Beyotime Cat# ST501 DMSO Sigma Cat# D2650 Critical commercial assays RaPure Total RNA Micro Kit Magen Cat# R4012 Whole RNA-seq Lib Prep kit ABclonal Cat# RK20303 mRNA-seq Lib Prep Kit ABclonal Cat# RK20302 Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 PrimeScript RT reagent Kit with gDNA Eraser Takara Cat# RR047B LIVE/DEAD Fixable Blue Dead Cell Stain Kit Invitrogen Cat# L34962 IL-1 beta Mouse Uncoated ELISA Kit Invitrogen Cat# 88-7013-88 Dual luciferase kit Promega Cat# N1110 Deposited data RNA_WT_DMSO_rep1 This paper GSM8581942 RNA_WT_DMSO_rep2 This paper GSM8581943 RNA_WT_DMSO_rep3 This paper GSM8581944 RNA_WT_Yoda1_rep1 This paper GSM8581945 RNA_WT_Yoda1_rep2 This paper GSM8581946 RNA_WT_Yoda1_rep3 This paper GSM8581947 RNA_Piezo1KO_DMSO_rep1 This paper GSM8581948 RNA_Piezo1KO_DMSO_rep2 This paper GSM8581949 RNA_Piezo1KO_DMSO_rep3 This paper GSM8581950 RNA_Piezo1KO_Yoda1_rep1 This paper GSM8581951 RNA_Piezo1KO_Yoda1_rep2 This paper GSM8581952 RNA_Piezo1KO_Yoda1_rep3 This paper GSM8581953 RNA_WT_PBS_rep1 This paper GSM8876047 RNA_WT_PBS_rep2 This paper GSM8876048 RNA_WT_PBS_rep3 This paper GSM8876049 RNA_WT_CA_rep1 This paper GSM8876050 RNA_WT_CA_rep2 This paper GSM8876051 RNA_WT_CA_rep3 This paper GSM8876052 RNA_Piezo1KO_DMSO_rep1 This paper GSM8876053 RNA_Piezo1KO_DMSO_rep2 This paper GSM8876054 RNA_Piezo1KO_DMSO_rep3 This paper GSM8876055 RNA_Piezo1KO_CA_rep1 This paper GSM8876056 RNA_Piezo1KO_CA_rep2 This paper GSM8876057 RNA_Piezo1KO_CA_rep3 This paper GSM8876058 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216; RRID: CVCL_0063 HeLa ATCC Cat#CCL-2; RRID: CVCL_0030 Experimental models: Organisms/strains Mouse:B6.Cg-Piezo1 tm2.1Apat /J Jackson Laboratory Stock No: 029213 Mouse:Lyz2-Cre Jackson Laboratory Stock No:004781 Mouse:NDR1 conditional knockout Generated by Biocytogen Co., Ltd (Beijing, China) N/A Mouse:NDR2 KOMP Repository Stock No:71464 (Continued on next page) Cell Reports 44, 115839, June 24, 2025 21

High Performance Liquid Chromatography:

Conditioned Place Preference:

Article Title: Systematic review of the subcutaneous air pouch model using monosodium urate and calcium pyrophosphate and recommendations for studying crystal-related arthropathies.
Article Snippet: .. pyrophosphate crystals While some published studies have obtained MSU (InvivoGen, Cat# tlrl- msu) and CPP (InvivoGen, Cat# tlrl- cppd) crystals from a supplier, the majority of authors synthetised their own MSU (55 of 81, 12 not specified) and CPP crystals (4 of 8, 3 not specified) according to methods described in the literature. ..

Article Title: Systematic review of the subcutaneous air pouch model using monosodium urate and calcium pyrophosphate and recommendations for studying crystal‐related arthropathies
Article Snippet: .. While some published studies have obtained MSU (InvivoGen, Cat# tlrl‐msu) and CPP (InvivoGen, Cat# tlrl‐cppd) crystals from a supplier, the majority of authors synthetised their own MSU (55 of 81, 12 not specified) and CPP crystals (4 of 8, 3 not specified) according to methods described in the literature. ..

Activation Assay:

Article Title: TRIM21 facilitates inflammasome assembly and contributes to autoinflammatory disease
Article Snippet: .. Cells were primed with ultrapure LPS from E. coli Serotype O111:B4 (1 μg mL −1 , Invivogen, tlrl-3pelps) for 3 h followed by activation with nigericin (10 μM, Invivogen, tlrl-nig) for 30 min, ATP (5 mM, Sigma-Aldrich, A6419) for 20 min, CPPD crystals (125 μg mL −1 , Invivogen, tlrl-cppd) for 6 h, silica crystals (400 μg mL −1 , Invivogen, tlrl-sio-2) for 6 h, Rho inhibitor TcdB (2 μg mL −1 , Cytoskeleton, CT04) for 4 h. Poly(dA:dT) (2 μg mL −1 , Invivogen, tlrl-patn-1), Bacillus anthracis Lethal Factor (1 μg mL −1 , List Biological Laboratories, 172 L), Protective Antigen (1 μg mL −1 , List Biological Laboratories, 171E), and ultrapure flagellin from Salmonella enterica subsp. enterica serovar Typhimurium (100 ng mL −1 , Invivogen, tlrl-epstfla) were transfected into THP-1 cells using Lipofectamine 2000 (0.8 μL/well, Invitrogen, 11668019) for 6 h or 16 h as indicated. ..

Transfection:

other:

Article Title: TRIM21 facilitates inflammasome assembly and contributes to autoinflammatory disease.
Article Snippet: THP-1 cells (3.3x105 cells per well) were electroporated (Neon, Invitrogen, MPK10096) with 60 nM siRNA duplexes.

Review