tlrl-cppd Search Results


cppd crystals  (InvivoGen)
94
InvivoGen cppd crystals
(A) Expression of ENPP1 by qPCR in injured and uninjured regions of hearts of B6 and C3H mice 7 days after cryo injury (mean± S.E.M., n=6, *p<0.01). (B–D) Immunofluorescent staining for ENPP1 in (B) uninjured B6 heart (C) injured B6 heart and (D) uninjured C3H heart (green, arrowheads). (E–G) Immunofluorescent staining of (E) ENPP1 in injured calcified regions of Col1a2Cre:R26Rtdtomato (C3H) heart (arrowheads) (F) ENPP1 expressing cells in higher magnification (G) tdTomato expressing fibroblasts in the same field and (H) merged image demonstrating expression of ENPP1 by tdTomato cells (arrowheads). (I) Confocal Raman microscopy demonstrating spectra of myocardial calcific deposits (blue line) compared to that of pure <t>CPPD</t> (black line) and hydroxyapatite (HA, red <t>line)</t> <t>crystals</t> (n=3). (J) Phosphate concentrations in injured and uninjured regions of non-calcified (B6) and calcified hearts (C3H) (mean±S.E.M.; n=6 animals; *p<0.01). (K–P) Cryo injured C3H animals treated with (K,M) vehicle or ENPP1 inhibitor (L,N) SYL-001 or (O,P) ARL67156 demonstrating calcium deposition on (K–O) gross inspection and (M–P) CT scan and following 3D reconstruction (ribs removed to visualize cardiac calcification in retrosternal region) (yellow and white arrow) (Q) Biochemical measurements of myocardial calcium deposits (n=6 in vehicle treated and n=9 animals for SYL-001 and n=5 or ARL67156, mean±S.E.M., *p<0.05 versus vehicle injected group). (R–U) Cryo-injured C3H animals treated with (R,S) normal saline or (T,U) etidronate demonstrating calcification on (R,T) gross inspection and (S,U) CT scan and following 3D reconstruction (yellow and white arrow). Note complete absence of any calcium deposits in the etidronate injected animals (representative images of n=3 for vehicle treated animals and n=6 for etidronate injected animals)
Cppd Crystals, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlrl-cppd/CPPD+crystals/pmc05291784-21-0-3
Average 94 stars, based on 1 article reviews
cppd crystals - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

Image Search Results


(A) Expression of ENPP1 by qPCR in injured and uninjured regions of hearts of B6 and C3H mice 7 days after cryo injury (mean± S.E.M., n=6, *p<0.01). (B–D) Immunofluorescent staining for ENPP1 in (B) uninjured B6 heart (C) injured B6 heart and (D) uninjured C3H heart (green, arrowheads). (E–G) Immunofluorescent staining of (E) ENPP1 in injured calcified regions of Col1a2Cre:R26Rtdtomato (C3H) heart (arrowheads) (F) ENPP1 expressing cells in higher magnification (G) tdTomato expressing fibroblasts in the same field and (H) merged image demonstrating expression of ENPP1 by tdTomato cells (arrowheads). (I) Confocal Raman microscopy demonstrating spectra of myocardial calcific deposits (blue line) compared to that of pure CPPD (black line) and hydroxyapatite (HA, red line) crystals (n=3). (J) Phosphate concentrations in injured and uninjured regions of non-calcified (B6) and calcified hearts (C3H) (mean±S.E.M.; n=6 animals; *p<0.01). (K–P) Cryo injured C3H animals treated with (K,M) vehicle or ENPP1 inhibitor (L,N) SYL-001 or (O,P) ARL67156 demonstrating calcium deposition on (K–O) gross inspection and (M–P) CT scan and following 3D reconstruction (ribs removed to visualize cardiac calcification in retrosternal region) (yellow and white arrow) (Q) Biochemical measurements of myocardial calcium deposits (n=6 in vehicle treated and n=9 animals for SYL-001 and n=5 or ARL67156, mean±S.E.M., *p<0.05 versus vehicle injected group). (R–U) Cryo-injured C3H animals treated with (R,S) normal saline or (T,U) etidronate demonstrating calcification on (R,T) gross inspection and (S,U) CT scan and following 3D reconstruction (yellow and white arrow). Note complete absence of any calcium deposits in the etidronate injected animals (representative images of n=3 for vehicle treated animals and n=6 for etidronate injected animals)

Journal: Cell stem cell

Article Title: Cardiac fibroblasts adopt osteogenic fates and can be targeted to attenuate pathological heart calcification

doi: 10.1016/j.stem.2016.10.005

Figure Lengend Snippet: (A) Expression of ENPP1 by qPCR in injured and uninjured regions of hearts of B6 and C3H mice 7 days after cryo injury (mean± S.E.M., n=6, *p<0.01). (B–D) Immunofluorescent staining for ENPP1 in (B) uninjured B6 heart (C) injured B6 heart and (D) uninjured C3H heart (green, arrowheads). (E–G) Immunofluorescent staining of (E) ENPP1 in injured calcified regions of Col1a2Cre:R26Rtdtomato (C3H) heart (arrowheads) (F) ENPP1 expressing cells in higher magnification (G) tdTomato expressing fibroblasts in the same field and (H) merged image demonstrating expression of ENPP1 by tdTomato cells (arrowheads). (I) Confocal Raman microscopy demonstrating spectra of myocardial calcific deposits (blue line) compared to that of pure CPPD (black line) and hydroxyapatite (HA, red line) crystals (n=3). (J) Phosphate concentrations in injured and uninjured regions of non-calcified (B6) and calcified hearts (C3H) (mean±S.E.M.; n=6 animals; *p<0.01). (K–P) Cryo injured C3H animals treated with (K,M) vehicle or ENPP1 inhibitor (L,N) SYL-001 or (O,P) ARL67156 demonstrating calcium deposition on (K–O) gross inspection and (M–P) CT scan and following 3D reconstruction (ribs removed to visualize cardiac calcification in retrosternal region) (yellow and white arrow) (Q) Biochemical measurements of myocardial calcium deposits (n=6 in vehicle treated and n=9 animals for SYL-001 and n=5 or ARL67156, mean±S.E.M., *p<0.05 versus vehicle injected group). (R–U) Cryo-injured C3H animals treated with (R,S) normal saline or (T,U) etidronate demonstrating calcification on (R,T) gross inspection and (S,U) CT scan and following 3D reconstruction (yellow and white arrow). Note complete absence of any calcium deposits in the etidronate injected animals (representative images of n=3 for vehicle treated animals and n=6 for etidronate injected animals)

Article Snippet: CPPD Crystals , InvivoGen , tlrl-cppd.

Techniques: Expressing, Staining, Microscopy, Computed Tomography, Injection, Saline

KEY RESOURCES TABLE

Journal: Cell stem cell

Article Title: Cardiac fibroblasts adopt osteogenic fates and can be targeted to attenuate pathological heart calcification

doi: 10.1016/j.stem.2016.10.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CPPD Crystals , InvivoGen , tlrl-cppd.

Techniques: Recombinant, Staining, Calcium Assay, Mineralization Assay, ALP Assay, RNA Sequencing Assay, Expressing, Real-time Polymerase Chain Reaction, Software, Imaging