Electrophoresis:Article Title: Inhibition of pancreatic cancer growth by an siRNA targeting SNORA22 and a folic acid-conjugated cationic oligopeptide.
Article Snippet: .. All samples were immediately flash-frozen in liquid nitrogen, and 15% native polyacrylamide gel (SuperSep DNA 15%, 17 wells, Fujifilm Wako Pure Chemical Industries, Osaka, Japan) electrophoresis was performed at 30 mA/each gel at rt for 67 min using EasySeparator (Fujifilm Wako Pure Chemical Industries, Osaka, Japan) and a Bio-Rad PowerPac HC power supply, followed by SYBR Gold staining (SYBR Gold Nucleic Acid Gel Stain, Invitrogen, Waltham, MA, USA) for 20 min. Each specimen was visualized using a BioDoc-It 220 Imaging System (UVP, Upland, CA, USA). ..
Article Title: An ultrasensitive CRISPR/Cas12a based electrochemical biosensor for detection of toxigenic Clostridioides difficile.
Article Snippet: Clostridioides difficile (C. difficile) infection (CDI) represents a formidable global healthcare challenge, necessitating the development of rapid, accurate, and cost-effective diagnostic platforms to mitigate nosocomial transmission and improve patient outcomes.. Compared with the conventional methods, CRISPR/Cas systems featured by specific target reorganization by a single chain RNA, coupled with electrochemical technology enables highly sensitive detection of various biomarkers.. However, their application to CDI has remained unexplored due to the lack of tailored crRNAs.
Staining:Article Title: Inhibition of pancreatic cancer growth by an siRNA targeting SNORA22 and a folic acid-conjugated cationic oligopeptide.
Article Snippet: .. All samples were immediately flash-frozen in liquid nitrogen, and 15% native polyacrylamide gel (SuperSep DNA 15%, 17 wells, Fujifilm Wako Pure Chemical Industries, Osaka, Japan) electrophoresis was performed at 30 mA/each gel at rt for 67 min using EasySeparator (Fujifilm Wako Pure Chemical Industries, Osaka, Japan) and a Bio-Rad PowerPac HC power supply, followed by SYBR Gold staining (SYBR Gold Nucleic Acid Gel Stain, Invitrogen, Waltham, MA, USA) for 20 min. Each specimen was visualized using a BioDoc-It 220 Imaging System (UVP, Upland, CA, USA). ..
Imaging:Article Title: Inhibition of pancreatic cancer growth by an siRNA targeting SNORA22 and a folic acid-conjugated cationic oligopeptide.
Article Snippet: .. All samples were immediately flash-frozen in liquid nitrogen, and 15% native polyacrylamide gel (SuperSep DNA 15%, 17 wells, Fujifilm Wako Pure Chemical Industries, Osaka, Japan) electrophoresis was performed at 30 mA/each gel at rt for 67 min using EasySeparator (Fujifilm Wako Pure Chemical Industries, Osaka, Japan) and a Bio-Rad PowerPac HC power supply, followed by SYBR Gold staining (SYBR Gold Nucleic Acid Gel Stain, Invitrogen, Waltham, MA, USA) for 20 min. Each specimen was visualized using a BioDoc-It 220 Imaging System (UVP, Upland, CA, USA). ..
Article Title: Chicken interleukin-26: characterization and antigen capture enzyme-linked immunosorbent assay
Article Snippet: The membranes were blocked with Superblock Blocking Buffer (Thermo Scientific, Waltham, MA) and incubated with 1 μg/ml of anti-chIL-26 mAbs (#5D11, #6C3, #7H10, #8A5, and #10C7). .. After incubation with horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG secondary antibody (Jackson ImmunoResearch Laboratories Inc., USA) diluted 1:1000 in blocking buffer, the membranes were visualized using Clarity Western ECL Substrate (Bio-Rad, add more product source) and detected using ChemiDoc Touch Imaging System (Bio-Rad, CA). ..
Article Title: An ultrasensitive CRISPR/Cas12a based electrochemical biosensor for detection of toxigenic Clostridioides difficile.
Article Snippet: Clostridioides difficile (C. difficile) infection (CDI) represents a formidable global healthcare challenge, necessitating the development of rapid, accurate, and cost-effective diagnostic platforms to mitigate nosocomial transmission and improve patient outcomes.. Compared with the conventional methods, CRISPR/Cas systems featured by specific target reorganization by a single chain RNA, coupled with electrochemical technology enables highly sensitive detection of various biomarkers.. However, their application to CDI has remained unexplored due to the lack of tailored crRNAs.
Protease Inhibitor:
Saline:
Transcranial Magnetic Stimulation:
cDNA Synthesis:
Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).
shRNA:
Sequencing:
Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).
Quantitative RT-PCR:
Recombinant:
Plasmid Preparation:
Incubation:Article Title: Chicken interleukin-26: characterization and antigen capture enzyme-linked immunosorbent assay
Article Snippet: The membranes were blocked with Superblock Blocking Buffer (Thermo Scientific, Waltham, MA) and incubated with 1 μg/ml of anti-chIL-26 mAbs (#5D11, #6C3, #7H10, #8A5, and #10C7). .. After incubation with horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG secondary antibody (Jackson ImmunoResearch Laboratories Inc., USA) diluted 1:1000 in blocking buffer, the membranes were visualized using Clarity Western ECL Substrate (Bio-Rad, add more product source) and detected using ChemiDoc Touch Imaging System (Bio-Rad, CA). ..
Blocking Assay:Article Title: Chicken interleukin-26: characterization and antigen capture enzyme-linked immunosorbent assay
Article Snippet: The membranes were blocked with Superblock Blocking Buffer (Thermo Scientific, Waltham, MA) and incubated with 1 μg/ml of anti-chIL-26 mAbs (#5D11, #6C3, #7H10, #8A5, and #10C7). .. After incubation with horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG secondary antibody (Jackson ImmunoResearch Laboratories Inc., USA) diluted 1:1000 in blocking buffer, the membranes were visualized using Clarity Western ECL Substrate (Bio-Rad, add more product source) and detected using ChemiDoc Touch Imaging System (Bio-Rad, CA). ..
Western Blot:Article Title: Chicken interleukin-26: characterization and antigen capture enzyme-linked immunosorbent assay
Article Snippet: The membranes were blocked with Superblock Blocking Buffer (Thermo Scientific, Waltham, MA) and incubated with 1 μg/ml of anti-chIL-26 mAbs (#5D11, #6C3, #7H10, #8A5, and #10C7). .. After incubation with horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG secondary antibody (Jackson ImmunoResearch Laboratories Inc., USA) diluted 1:1000 in blocking buffer, the membranes were visualized using Clarity Western ECL Substrate (Bio-Rad, add more product source) and detected using ChemiDoc Touch Imaging System (Bio-Rad, CA). ..
Nucleic Acid Electrophoresis:Article Title: An ultrasensitive CRISPR/Cas12a based electrochemical biosensor for detection of toxigenic Clostridioides difficile.
Article Snippet: Clostridioides difficile (C. difficile) infection (CDI) represents a formidable global healthcare challenge, necessitating the development of rapid, accurate, and cost-effective diagnostic platforms to mitigate nosocomial transmission and improve patient outcomes.. Compared with the conventional methods, CRISPR/Cas systems featured by specific target reorganization by a single chain RNA, coupled with electrochemical technology enables highly sensitive detection of various biomarkers.. However, their application to CDI has remained unexplored due to the lack of tailored crRNAs.
Reverse Transcription Polymerase Chain Reaction:Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).
Single Cell RNA-Seq:Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).
Analysis:Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).
Gene Expression:Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).
RNA Sequencing:Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).
Software:Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).
SDS Page:Article Title: MED26 facilitates Mediator-Pol II complex assembly and promotes nuclear stress body formation during heat stress.
Article Snippet: .. Samples were boiled at 95◦C for 5 min, loaded onto 10% Mini-PROTEAN TGX gels (Bio-Rad), and separated by SDS-PAGE in running buffer (192 mM glycine, 25 mM Trizmabase, 0.1% SDS) at 20 mA/gel for 50 min using a PowerPac Basic power supply (Bio-Rad). .. Proteins were then transferred to Immobilon-P polyvinylidene fluoride membranes (IPVH00005; Millipore, Burlington, MA, USA) that had been iScience 29, 117557, October 16, 2026 23 preactivated in methanol for 5 min.
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