snu387  (ATCC)

Journal: bioRxiv

Article Title: Synergistic role of riboflavin-auxotrophic Enterococcus for MR1 expression and intra-tumoral mucosal associated invariant T (MAIT) cell activation

doi: 10.1101/2025.11.25.690539

Figure Lengend Snippet: A, J33-4 MAIT TCR recognition of U266 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU, in presence or not of E.hirae , E. faecalis , E. faecium , E. coli and B. longum (Left graph) and E. avium (right graph, independent experiment). TCR activation is measured by bioluminescence reading. B , different APC (human dendritic cells, THP1, melanoma MeWO and hepatocellular carcinoma SNU387) were tested for the synergistic effect of E faecalis onto activation of conventional MAIT TCR J33-4 in presence (inverse black triangles) or not (black circles) of 5-OP-RU. C , Flow cytometry analysis of MR1 surface membrane expression using the 26.5 anti MR1 antibody staining of U266, THP1 and dendritic cells (DC) incubated overnight without or with 5-OP-RU in presence or not of E.faecalis . D , Inhibition by Ac-6-FP (escalating doses from 0.2 to 200 uM) of recognition by MAIT TCR J33-4 of MR1 expressed on U266 cultured or not with 5-OP-RU (6.25 uM) in presence (triangles) or not (squares) of E.faecalis (MOI:125). E , MAIT TCR J33-4 activation by Myd88 -/- THP1 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU (6.25 uM), in presence or not of E. faecalis (MOI:125). F , MAIT TCR J33-4 activation by U266 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU (6.25 uM), in presence or not of E. faecalis (MOI:125) and E.faecalis derived outer membrane vesicles (OMV). G , Flow cytometry analysis of MR1 surface membrane expression using the 26.5 anti MR1 antibody staining of U266 incubated overnight without or with 5-OP-RU in presence or not of E. faecalis (MOI:125), compared to LPS (TLR4 ligand; 50ng/ml; left histogram), CPG (TLR9 ligand; 5uM, middle histogram) or LTA (TLR2 ligand; 30ug/ml co; right histogram). All data are shown as mean ± SEM with technical replicates represented by number of symbols in each graph. ****:P<0.0001, ***:0.0001

Article Snippet: MeWo and SNU387 cell lines were kindly provided by Dr. Scott Wilson’s lab and cul-tured according to ATCC protocol.

Techniques: Incubation, Activation Assay, Flow Cytometry, Membrane, Expressing, Staining, Inhibition, Cell Culture, Derivative Assay, Two Tailed Test

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: MiR-423-5p is a metabolic and growth tuner in hepatocellular carcinoma via MALAT-1 and mitochondrial interaction

doi: 10.1186/s13046-025-03524-2

Figure Lengend Snippet: A Basal expression levels of miR-423-5p and MALAT-1 across seven HCC cell lines (SNU449, HepG2, SNU475, Hep3B, SKHep1, SNU387, and SNU389) assessed by qRT-PCR. Expression values were normalized to endogenous controls and used to guide selection of models for functional assays. B Generation of stable miR-423-5p-overexpressing clones in HepG2, Hep3B, and SNU387 cells using lentiviral vectors expressing GFP. Puromycin selection was applied to enrich successfully transduced cells. qRT-PCR confirmed persistent miR-423-5p upregulation. C Stable overexpression of miR-423-5p led to a consistent downregulation of MALAT-1 levels compared to vector-only controls in all three cell models. D – E Establishment of stable MALAT-1-overexpressing clones in SNU387 and Hep3B cells via lentiviral transduction. Overexpression was confirmed by qRT-PCR. In the selected clones (SNU387 clone 1 and Hep3B clone 2), MALAT-1 upregulation was associated with reduced miR-423-5p levels, confirming a reciprocal regulatory relationship. * \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$p < 0.05$$\end{document} p < 0.05 , ** p -value \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$< 0.01$$\end{document} < 0.01 , *** p -value \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$< 0.001$$\end{document} < 0.001 , **** p -value \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$< 0.0001$$\end{document} < 0.0001

Article Snippet: We obtained HepG2 and SNU387 HCC cell lines from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR, Selection, Functional Assay, Clone Assay, Over Expression, Plasmid Preparation, Transduction

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: MiR-423-5p is a metabolic and growth tuner in hepatocellular carcinoma via MALAT-1 and mitochondrial interaction

doi: 10.1186/s13046-025-03524-2

Figure Lengend Snippet: miR-423-5p overexpression reduces proliferation in HCC cell lines ( A - C ), while MALAT-1 overexpression alone does not affect growth ( D - E ). Live-cell monitoring of cell confluence using the IncuCyte system in Hep3B and SNU387 models seeded at two different densities ( F – H ). miR-423-5p-overexpressing cells exhibited markedly reduced confluence compared to control cells over time. * \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$p < 0.05$$\end{document} p < 0.05 , ** p -value \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$< 0.01$$\end{document} < 0.01 , *** p -value \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$< 0.001$$\end{document} < 0.001 , **** p -value \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$< 0.0001$$\end{document} < 0.0001

Article Snippet: We obtained HepG2 and SNU387 HCC cell lines from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Over Expression, Control

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: MiR-423-5p is a metabolic and growth tuner in hepatocellular carcinoma via MALAT-1 and mitochondrial interaction

doi: 10.1186/s13046-025-03524-2

Figure Lengend Snippet: A Wound healing assay performed in HepG2, Hep3B, and SNU387 cell lines stably overexpressing miR-423-5p vs empty vector (Left side). Wound healing assay performed in Hep3B, and SNU387 cell lines stably overexpressing MALAT-1 vs empty vector (Right side). B Transwell migration and invasion assay using Cultrex BME-coated inserts. miR-423-5p overexpression significantly decreased the number of migrating and invading cells after 48 hours compared to control cells (Left Side). MALAT-1 overexpression is remarkably boosting migrating and invasive capability of HEP3B and SNU387 (Right side). C Colony formation assay under low-density and semi-starved conditions for 14 days revealed impaired clonogenicity in all miR-423-5p-overexpressing HCC cell models compared to respective controls. * \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$p < 0.05$$\end{document} p < 0.05 , ** p -value \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$< 0.01$$\end{document} < 0.01 , *** p -value \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$< 0.001$$\end{document} < 0.001 , **** p -value \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$< 0.0001$$\end{document} < 0.0001

Article Snippet: We obtained HepG2 and SNU387 HCC cell lines from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Wound Healing Assay, Stable Transfection, Plasmid Preparation, Migration, Invasion Assay, Over Expression, Control, Colony Assay

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: MiR-423-5p is a metabolic and growth tuner in hepatocellular carcinoma via MALAT-1 and mitochondrial interaction

doi: 10.1186/s13046-025-03524-2

Figure Lengend Snippet: A Representative fluorescence microscopy images of mitochondria in SNU387 clones expressing either miR-423-5p or MALAT-1, transfected with MTS-mCherry-GFP1-10 plasmid. miR-423-5p-overexpressing cells showed fewer and smaller mitochondria with a rounded morphology, whereas MALAT-1-overexpressing cells displayed increased mitochondrial number and size. B qRT-PCR analysis of mitochondrial-related gene expression in SNU387 clones. Genes encoded by both mitochondrial and nuclear DNA involved in mitochondrial respiration and activity were significantly downregulated in miR-423-5p-overexpressing cells and upregulated in MALAT-1-overexpressing clones. C Seahorse Mito Stress Test performed in miR-423-5p-overexpressing SNU387 and Hep3B cells revealed reduced mitochondrial function, including decreased basal respiration, maximal respiration, ATP production, proton leak, and spare respiratory capacity, compared to control cells. * \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$p < 0.05$$\end{document} p < 0.05 , ** p -value \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$< 0.01$$\end{document} < 0.01 , *** p -value \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$< 0.001$$\end{document} < 0.001 , **** p -value \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$< 0.0001$$\end{document} < 0.0001 .

Article Snippet: We obtained HepG2 and SNU387 HCC cell lines from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Fluorescence, Microscopy, Clone Assay, Expressing, Transfection, Plasmid Preparation, Quantitative RT-PCR, Gene Expression, Activity Assay, Control