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Image Search Results
Journal: Bioengineered
Article Title: Vacuolar protein sorting-associated protein 72 homolog (VPS72) binding to lysine acetyltransferase 5 (KAT5) promotes the proliferation, invasion and migration of hepatocellular carcinoma through regulating phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway
doi: 10.1080/21655979.2022.2056692
Figure Lengend Snippet: VPS72 was highly expressed in hepatocellular carcinoma tissues and cell lines and correlated with poor prognosis. (a-b) High expression of VPS72 in hepatocellular carcinoma tissues and low overall survival of patients with hepatocellular carcinoma were showed on GEPIA2 database. (c-e) Major signaling pathways where VPS72 was enriched were presented on GSEA software. (f-g) Protein and mRNA expression levels of VPS72 were tested by western blot and RT-qPCR in the human immortalized liver cell lines MIHA, human hepatocellular carcinoma cells lines Hep10, HuH-7 and SNU-387. Results were generated from three independent experiments and data were expressed as mean ± standard deviation (SD). *P < 0.05, **P < 0.01, ***P < 0.001.
Article Snippet: The human immortalized
Techniques: Expressing, Protein-Protein interactions, Software, Western Blot, Quantitative RT-PCR, Generated, Standard Deviation
Journal: Bioengineered
Article Title: Vacuolar protein sorting-associated protein 72 homolog (VPS72) binding to lysine acetyltransferase 5 (KAT5) promotes the proliferation, invasion and migration of hepatocellular carcinoma through regulating phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway
doi: 10.1080/21655979.2022.2056692
Figure Lengend Snippet: VPS72 could interact with KAT5 in hepatocellular carcinoma cells. (a-b) The interaction of VPS72 and KAT5 was analyzed by bioGRID database and STRING database, respectively. (c) The correlation analysis between VPS72 and KAT5 expression in hepatocellular carcinoma tissues was analyzed by GEPIA2 database. (d-e) Protein and mRNA expression levels of KAT5 were detected by western blot and RT-qPCR in the human immortalized liver cell lines MIHA, human hepatocellular carcinoma cells lines Hep10, HuH-7 and SNU-387. (f) The targeted binding of KAT5 and VPS72 was confirmed by Co-IP. (g) KAT5 expression in HuH7 cells in the groups of control, sh-NC, and sh-VPS72#2 was tested by western blot after VPS72 knockdown. Experiments were repeated three times and the measurement data were expressed in the form of the mean ± standard deviation (SD). **P < 0.01, ***P < 0.001.
Article Snippet: The human immortalized
Techniques: Expressing, Western Blot, Quantitative RT-PCR, Binding Assay, Co-Immunoprecipitation Assay, Control, Knockdown, Standard Deviation