Journal: bioRxiv
Article Title: Smoothened turnover regulated by Hedgehog signaling in Drosophila
doi: 10.64898/2026.01.08.698469
Figure Lengend Snippet: Wild-type wing disc stained with α-Smo (red) and α-Ptc (green) at apical (A,A’) and basolateral (B,B’) optical planes. (A,B) Smo and Ptc staining; (A’,B’) Smo staining only. (A”,B”) Graphs showing intensity plots of images in (A,B) across A/P axis. (C,D) Same as (A,B) for unfixed disc expressing BAC-encoded GFP:Smo and mIFP driven by ptc-Gal4 showing fluorescence of Smo (green) and mIFP (blue) ( ptc-Gal4>mIFP ; GFP: Smo / + ). (E,F) Same as (C,D) for disc expressing BAC-encoded Cherry:Smo (red) and mIFP driven by ptc-Gal4 ( ptc-Gal4>mIFP ; Cherry : Smo / + ). (G) Same as (F) showing membrane-localized fluorescence only.
Article Snippet: The following antibodies were used: mouse α-GFP (Roche), rabbit α-RFP (Rockland), mouse α-Ptc (DSHB, Apa1), mouse α-En (DSHB, 4D9), DAPI, Phalloidin, mouse α-Dlg1, (DSHB, 4F3), α-Rab7 , α-Smo (DSHB, 20C6), Alexa633 conjugated-Phalloidin (Invitrogen), DAPI (Invitrogen), AbN ( ).
Techniques: Staining, Expressing, Fluorescence, Membrane