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Image Search Results
Journal: The Journal of Bone and Joint Surgery. American volume
Article Title: Sclerostin Antibody Treatment Enhances Rotator Cuff Tendon-to-Bone Healing in an Animal Model
doi: 10.2106/JBJS.16.01019
Figure Lengend Snippet: Names of Genes, Associated Categories, and TaqMan Assay Identification Numbers *
Article Snippet: Results are presented as relative expression compared with Rpl13a expression (2 -ΔCt ). table ft1 table-wrap mode="anchored" t5 TABLE II caption a7 Gene Name Category TaqMan Assay ID Tnfrsf11b (tumor necrosis factor receptor superfamily, member 11b), OPG (osteoprotegerin) Bone, inhibits osteoclast activity Rn00563499_m1 Sost (sclerostin) Bone, osteoclast activity Rn00577971_m1 Dmp1 (dentin matrix acidic phosphoprotein) Bone, osteoclast activity Rn01450122_m1 RankL (receptor activator of nuclear factor kappa-B ligand), Tnfsf11 (tumor necrosis factor [ligand] superfamily, member 11) Bone, osteoblast activity Rn00589289_m1 Bglap (bone gamma-carboxyglutamate protein), OCN (osteocalcin) Bone, osteoblast activity Rn00566386_g1 Pth1r (parathyroid hormone 1 receptor) Bone, osteoblast activity Rn00571596_m1 Ctsk (cathepsin K) Bone, bone resorption Rn00580723_m1 Dkk1 (dickkopf WNT signaling pathway inhibitor 1) Bone, Wnt signaling antagonist Rn01501537_m1 Lrp5 (low density lipoprotein receptor-related protein 5) Bone, Wnt signaling Rn01451428_m1 Runx2 (runt-related transcription factor 2) Bone, osteoprogenitor Rn01512298_m1 Sp7 (Osterix) Bone, osteoprogenitor Rn02769744_s1 Scx (scleraxis) Tendon Rn01504576_m1 Tnmd (tenomodulin) Tendon Rn00574164_m1 Col1a1 (collagen, type I, alpha 1) Tendon Rn01463848_m1 Col1a2 (collagen, type I, alpha 2) Tendon Rn01526721_m1 Col3a1 (collagen, type III, alpha 1) Tendon Rn01437681_m1 Col2a1 (collagen, type II, alpha 1) Fibrocartilage Rn01637087_m1 Acan (aggrecan) Fibrocartilage Rn00573424_m1 Tgfb1 (transforming growth factor, beta 1) Fibrocartilage Rn00572010_m1 Tgfb3 (transforming growth factor, beta 3) Fibrocartilage Rn00565937_m1 Mmp2 (matrix metalloproteinase 2) Fibrocartilage Rn01538170_m1 Sox9 (SRY [sex determining region Y]-box 9) Fibrocartilage Rn01751069_mH Smo (smoothened, frizzled class receptor) Development
Techniques: TaqMan Assay, Activity Assay
Journal: Scientific Reports
Article Title: Rab1A promotes cell proliferation and migration by upregulating Gli1 in colorectal cancer
doi: 10.1038/s41598-021-95798-7
Figure Lengend Snippet: Rab1A upregulated Gli1 expression in an SMO-independent manner. ( A – C ). Gli1 and SMO expression in Rab1A-silenced cells as detected by Western blotting ( A ). The bands were quantified and presented as the mean ± S.E.M of three independent experiments ( B and C ). ( D – F ). Gli1 and SMO expression in Rab1A-overexpressed cells as detected by Western blotting ( D ). The bands were quantified and presented as the mean ± S.E.M of three independent experiments ( E and F ). ( G ) Gli1 expression is detected by Western blotting in Rab1A-overexpressed SW480 cells treated with cyclopamine (Cyc; 5 μM) for 24 h. Here cropped blots were displayed and all full-length blots are included in the . ns, nonsignificant, ** P < 0.01.
Article Snippet: Primary antibodies used for immunoblotting were listed as followings: anti-E-cadherin antibody (diluted at 1:1000; 20874-1-AP; Proteintech), anti-Vimentin antibody (diluted at 1:1000; 10366-1-AP; Proteintech), anti-Gli1 antibody (diluted at 1:1000; 66905-1-Ig; Proteintech),
Techniques: Expressing, Western Blot
Journal: Scientific Reports
Article Title: Rab1A promotes cell proliferation and migration by upregulating Gli1 in colorectal cancer
doi: 10.1038/s41598-021-95798-7
Figure Lengend Snippet: Rab1A regulated the phosphorylation of mTOR in CRC cells. ( A ) p-mTOR and mTOR expression in Rab1A-silenced cells as detected by Western blotting. ( B ) p-mTOR and mTOR expression in Rab1A-overexpressed cells as detected by Western blotting. ( C ) A proposed model for SMO-independent Gli1 upregulation regulated by Rab1A. Here cropped blots were displayed and all full-length blots are included in the . * P < 0.05, ** P < 0.01.
Article Snippet: Primary antibodies used for immunoblotting were listed as followings: anti-E-cadherin antibody (diluted at 1:1000; 20874-1-AP; Proteintech), anti-Vimentin antibody (diluted at 1:1000; 10366-1-AP; Proteintech), anti-Gli1 antibody (diluted at 1:1000; 66905-1-Ig; Proteintech),
Techniques: Phospho-proteomics, Expressing, Western Blot