s schenckii s str  (ATCC)

Journal: Brazilian Journal of Microbiology

Article Title: Effects of metaperiodate and urea solutions on the serological diagnosis of human sporotrichosis using an indirect ELISA test

doi: 10.1007/s42770-018-0005-6

Figure Lengend Snippet: ELISA results of IgG (means and standard deviations of O.D. at 490 nm) obtained from the sera from patients with sporotrichosis (Ss+, n = 8), sera from individuals with sporotrichin-negative skin tests (Ss−, n = 8), and sera from patients with paracoccidioidomycosis (PCM, n = 8) against yeast-phase S. schenckii exoantigens. Comparison using the Kruskal-Wallis multivariate analysis, followed by a Dunn’s post-test, was made between Ss+, Ss−, and PCM, for each dilution. *P < 0.05

Article Snippet: Production of crude exoantigens of Sporothrix schenckii S. schenckii s. str exoantigens prepared from the yeast phase of the strain #118 (isolated from a human case of lymphocutaneous sporotrichosis) were obtained, as previously described, using Sabouraud medium (Difco) [ 15 ].

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Brazilian Journal of Microbiology

Article Title: Effects of metaperiodate and urea solutions on the serological diagnosis of human sporotrichosis using an indirect ELISA test

doi: 10.1007/s42770-018-0005-6

Figure Lengend Snippet: Receiver operating characteristic (ROC) curves for the crude S. schenckii exoantigens comparing different dilutions of sera in the presence or absence of sodium metaperiodate solution. The ROC is plotted between the true-positive rate (sensitivity) on the y-axis, and the false-positive rate (1-specificity) on the x-axis. The area under the curve (AUC) represents the accuracy of the ELISA test, which was 0.9

Article Snippet: Production of crude exoantigens of Sporothrix schenckii S. schenckii s. str exoantigens prepared from the yeast phase of the strain #118 (isolated from a human case of lymphocutaneous sporotrichosis) were obtained, as previously described, using Sabouraud medium (Difco) [ 15 ].

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Brazilian Journal of Microbiology

Article Title: Effects of metaperiodate and urea solutions on the serological diagnosis of human sporotrichosis using an indirect ELISA test

doi: 10.1007/s42770-018-0005-6

Figure Lengend Snippet: Receiver operating characteristic (ROC) curves for the crude S. schenckii exoantigens comparing different sera dilutions and incubation periods, in the presence or absence of 6 M urea solution. The ROC is plotted between the true-positive rate (sensitivity) on the y-axis, and the false-positive rate (1-specificity) on the x-axis. The area under the curve (AUC) represents the accuracy of the ELISA test, which was 0.9

Article Snippet: Production of crude exoantigens of Sporothrix schenckii S. schenckii s. str exoantigens prepared from the yeast phase of the strain #118 (isolated from a human case of lymphocutaneous sporotrichosis) were obtained, as previously described, using Sabouraud medium (Difco) [ 15 ].

Techniques: Incubation, Enzyme-linked Immunosorbent Assay

Journal: Brazilian Journal of Microbiology

Article Title: Effects of metaperiodate and urea solutions on the serological diagnosis of human sporotrichosis using an indirect ELISA test

doi: 10.1007/s42770-018-0005-6

Figure Lengend Snippet: ELISA results of IgG (fold increased cutoff) obtained from the sera of patients with sporotrichosis (Ss+, n = 8), sera from individuals with sporotrichin-negative skin tests (Ss−, n = 8), sera from patients with paracoccidioidomycosis (PCM, n = 8), and test sera from patients with different clinical forms of sporotrichosis (test, n = 20) against yeast-phase S. schenckii exoantigens. Comparison using the Kruskal-Wallis multivariate analysis, followed by a Dunn’s post-test. Ss− compared to PCM, and Ss+ compared to test, P > 0.05; Ss− and PCM compared to Ss+ and test, P < 0.05. Cutoff values for serum reactivity were calculated by dividing the mean of the optical density readings of each serum sample at 1/1600 dilution by negative samples in the presence of 6 M urea solution

Article Snippet: Production of crude exoantigens of Sporothrix schenckii S. schenckii s. str exoantigens prepared from the yeast phase of the strain #118 (isolated from a human case of lymphocutaneous sporotrichosis) were obtained, as previously described, using Sabouraud medium (Difco) [ 15 ].

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Brazilian Journal of Microbiology

Article Title: Effects of metaperiodate and urea solutions on the serological diagnosis of human sporotrichosis using an indirect ELISA test

doi: 10.1007/s42770-018-0005-6

Figure Lengend Snippet: ELISA results of IgG (means and standard deviations of O.D. at 490 nm) obtained from the sera from patients with sporotrichosis (Ss+, n = 8), sera from individuals with sporotrichin-negative skin tests (Ss−, n = 8), and sera from patients with paracoccidioidomycosis (PCM, n = 8) against yeast-phase S. schenckii exoantigens. Comparison using the Kruskal-Wallis multivariate analysis, followed by a Dunn’s post-test, was made between Ss+, Ss−, and PCM, for each dilution. *P < 0.05

Article Snippet: S. schenckii s. str exoantigens prepared from the yeast phase of the strain #118 (isolated from a human case of lymphocutaneous sporotrichosis) were obtained, as previously described, using Sabouraud medium (Difco) [ 15 ].

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Brazilian Journal of Microbiology

Article Title: Effects of metaperiodate and urea solutions on the serological diagnosis of human sporotrichosis using an indirect ELISA test

doi: 10.1007/s42770-018-0005-6

Figure Lengend Snippet: Receiver operating characteristic (ROC) curves for the crude S. schenckii exoantigens comparing different dilutions of sera in the presence or absence of sodium metaperiodate solution. The ROC is plotted between the true-positive rate (sensitivity) on the y-axis, and the false-positive rate (1-specificity) on the x-axis. The area under the curve (AUC) represents the accuracy of the ELISA test, which was 0.9

Article Snippet: S. schenckii s. str exoantigens prepared from the yeast phase of the strain #118 (isolated from a human case of lymphocutaneous sporotrichosis) were obtained, as previously described, using Sabouraud medium (Difco) [ 15 ].

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Brazilian Journal of Microbiology

Article Title: Effects of metaperiodate and urea solutions on the serological diagnosis of human sporotrichosis using an indirect ELISA test

doi: 10.1007/s42770-018-0005-6

Figure Lengend Snippet: Receiver operating characteristic (ROC) curves for the crude S. schenckii exoantigens comparing different sera dilutions and incubation periods, in the presence or absence of 6 M urea solution. The ROC is plotted between the true-positive rate (sensitivity) on the y-axis, and the false-positive rate (1-specificity) on the x-axis. The area under the curve (AUC) represents the accuracy of the ELISA test, which was 0.9

Article Snippet: S. schenckii s. str exoantigens prepared from the yeast phase of the strain #118 (isolated from a human case of lymphocutaneous sporotrichosis) were obtained, as previously described, using Sabouraud medium (Difco) [ 15 ].

Techniques: Incubation, Enzyme-linked Immunosorbent Assay

Journal: Brazilian Journal of Microbiology

Article Title: Effects of metaperiodate and urea solutions on the serological diagnosis of human sporotrichosis using an indirect ELISA test

doi: 10.1007/s42770-018-0005-6

Figure Lengend Snippet: ELISA results of IgG (fold increased cutoff) obtained from the sera of patients with sporotrichosis (Ss+, n = 8), sera from individuals with sporotrichin-negative skin tests (Ss−, n = 8), sera from patients with paracoccidioidomycosis (PCM, n = 8), and test sera from patients with different clinical forms of sporotrichosis (test, n = 20) against yeast-phase S. schenckii exoantigens. Comparison using the Kruskal-Wallis multivariate analysis, followed by a Dunn’s post-test. Ss− compared to PCM, and Ss+ compared to test, P > 0.05; Ss− and PCM compared to Ss+ and test, P < 0.05. Cutoff values for serum reactivity were calculated by dividing the mean of the optical density readings of each serum sample at 1/1600 dilution by negative samples in the presence of 6 M urea solution

Article Snippet: S. schenckii s. str exoantigens prepared from the yeast phase of the strain #118 (isolated from a human case of lymphocutaneous sporotrichosis) were obtained, as previously described, using Sabouraud medium (Difco) [ 15 ].

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Virulence

Article Title: Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species

doi: 10.4161/viru.23112

Figure Lengend Snippet: Figure 3. Colony count (CFU/g) comparison among different strains of S. schenckii s. str., and S. brasiliensis or S. globosa in spleen (A), lungs (B) and liver (C) obtained from infected BALB/c mice. Wide bars represent the mean CFU/g of tissue recovered from five mice and thin bars represent the standard deviation. *p < 0.05.

Article Snippet: Exoantigens from mycelial phase of S. schenckii s. str., S. globosa and S. brasiliensis isolates were obtained as previous described 43 using Sabouraud medium (Difco).

Techniques: Infection, Standard Deviation

Journal: Virulence

Article Title: Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species

doi: 10.4161/viru.23112

Figure Lengend Snippet: Figure 4. (A) Protein/glycoprotein profile of exoantigens of S. schenckii, S. brasiliensis and S. globosa, detected by SDS-PAGE. (B) Immunogenic molecules from exoantigens recognized by antisera from infected animals—western blot assay. S. brasiliensis isolate Ss54 (CBS 132990); S. globosa isolate Ss06 (CBS 132922) and S. schenckii s. str. isolates Ss16, Ss39, Ss40, Ss47, Ss51, Ss 126, Ss141 and Ss143.

Article Snippet: Exoantigens from mycelial phase of S. schenckii s. str., S. globosa and S. brasiliensis isolates were obtained as previous described 43 using Sabouraud medium (Difco).

Techniques: SDS Page, Infection, Western Blot