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ImmunoTools rhil-4
Rhil 4, supplied by ImmunoTools, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhil-4/rhil+4/pm40559654-171-15-16
Average 90 stars, based on 1 article reviews
rhil-4 - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Ex Vivo:

Article Title: The mycotoxin Beauvericin is an uncompetitive inhibitor of Cathepsin B.
Article Snippet: iDCs were generated according to the protocol (Holken and Teusch 2023), namely, 1 106 THP-1 cells were cultured in a T25 flask with 5 mL RPMI (Thermo Fisher Scientific, Cat# 31870-025) including 10% heat-inactivated FCS (Sigma-Aldrich, Cat# F7524), 0.1% 2-mercaptoethanol (Thermo Fischer Scientific, Cat# 31350-010), 1% PenStrep (Biochrom GmbH, Cat# A2212), 1500 IU/mL rhGM-CSF (ImmunoTools, Cat# 11343125), and 1500 IU/mL rhIL-4 (ImmunoTools, Cat# 11340045) and incubated at 37 C and 5% CO2 for 5 days.

Article Title: Pro-inflammatory macrophages suppress HIV replication in humanized mice and ex vivo co-cultures.
Article Snippet: Pro-inflammatory M1 polarization was achieved by adding 20 ng/ml rhIFNg (Immunotools, Cat#: 11343534) and 100 ng/ml LPS (SigmaAldrich, Cat#: L2387) for an additional 2 days, and anti-inflammatory M2 polarization by adding 20 ng/ml rhIL-4 (Immunotools, Cat#: 11340043).

Article Title: The mycotoxin Beauvericin is an uncompetitive inhibitor of Cathepsin B
Article Snippet: iDCs were generated according to the protocol (Holken and Teusch ), namely, 1 × 10 6 THP‐1 cells were cultured in a T25 flask with 5 mL RPMI (Thermo Fisher Scientific, Cat# 31870‐025) including 10% heat‐inactivated FCS (Sigma‐Aldrich, Cat# F7524), 0.1% 2‐mercaptoethanol (Thermo Fischer Scientific, Cat# 31350‐010), 1% PenStrep (Biochrom GmbH, Cat# A2212), 1500 IU/mL rhGM‐CSF (ImmunoTools, Cat# 11343125), and 1500 IU/mL rhIL‐4 (ImmunoTools, Cat# 11340045) and incubated at 37°C and 5% CO 2 for 5 days.

Article Title: The mycotoxin Beauvericin is an uncompetitive inhibitor of Cathepsin B
Article Snippet: On day 3, 4 ml of the medium was exchanged for fresh medium including 1500 IU/ml rhGM-CSF (ImmunoTools, Cat#11343125) and 1500 IU/ml rhIL-4 (ImmunoTools, Cat#11340045).

Article Title: CSF1R + myeloid-monocytic cells drive CAR-T cell resistance in aggressive B cell lymphoma.
Article Snippet: For differentiation of ex vivo generated human CSF1R+CD14+CD68+ LAMM cells we followed a recently established protocol.37 Here we cultivated cells in equal volumes of lymphoma-conditioned media and HAB media (RPMI + 10% human AB-Serum + L-Glutamin + 1% Penicillin/Streptomycin) supplemented with 1 μg/mL of rhIL-4, rhIL-10 and rhM-CSF (all ImmunoTools).

Article Title: Pro-inflammatory macrophages suppress HIV replication in humanized mice and ex vivo co-cultures
Article Snippet: Pro-inflammatory M1 polarization was achieved by adding 20 ng/ml rhIFNγ (Immunotools, Cat#: 11343534) and 100 ng/ml LPS (Sigma Aldrich, Cat#: L2387) for an additional 2 days, and anti-inflammatory M2 polarization by adding 20 ng/ml rhIL-4 (Immunotools, Cat#: 11340043).

Article Title: EFSA Project on the use of NAMs to explore the immunotoxicity of PFAS
Article Snippet: THP-1 cells (1x105 cells/mL) were treated for 5 days with rhIL-4 (1500 UI/mL — ImmunoTools GmbH, Friesoythe, Germany) and rhGM-CSF (1500 UI/mL — ImmunoTools GmbH) to acquire the properties of immature DCs (iDCs) as described by Berges et al. (2005).

Article Title: Pseudopterosin A-D Modulates Dendritic Cell Activation in Skin Sensitization.
Article Snippet: Differentiation was induced by adding 1500 IU/mL rhGM-CSF (ImmunoTools, #11343125, Friesoythe, Germany) and 1500 IU/mL rhIL-4 (ImmunoTools, #11340045), with a medium change performed on day 3.

Generated:

Article Title: The mycotoxin Beauvericin is an uncompetitive inhibitor of Cathepsin B.
Article Snippet: iDCs were generated according to the protocol (Holken and Teusch 2023), namely, 1 106 THP-1 cells were cultured in a T25 flask with 5 mL RPMI (Thermo Fisher Scientific, Cat# 31870-025) including 10% heat-inactivated FCS (Sigma-Aldrich, Cat# F7524), 0.1% 2-mercaptoethanol (Thermo Fischer Scientific, Cat# 31350-010), 1% PenStrep (Biochrom GmbH, Cat# A2212), 1500 IU/mL rhGM-CSF (ImmunoTools, Cat# 11343125), and 1500 IU/mL rhIL-4 (ImmunoTools, Cat# 11340045) and incubated at 37 C and 5% CO2 for 5 days.

Article Title: Pro-inflammatory macrophages suppress HIV replication in humanized mice and ex vivo co-cultures.
Article Snippet: Pro-inflammatory M1 polarization was achieved by adding 20 ng/ml rhIFNg (Immunotools, Cat#: 11343534) and 100 ng/ml LPS (SigmaAldrich, Cat#: L2387) for an additional 2 days, and anti-inflammatory M2 polarization by adding 20 ng/ml rhIL-4 (Immunotools, Cat#: 11340043).

Article Title: The mycotoxin Beauvericin is an uncompetitive inhibitor of Cathepsin B
Article Snippet: iDCs were generated according to the protocol (Holken and Teusch ), namely, 1 × 10 6 THP‐1 cells were cultured in a T25 flask with 5 mL RPMI (Thermo Fisher Scientific, Cat# 31870‐025) including 10% heat‐inactivated FCS (Sigma‐Aldrich, Cat# F7524), 0.1% 2‐mercaptoethanol (Thermo Fischer Scientific, Cat# 31350‐010), 1% PenStrep (Biochrom GmbH, Cat# A2212), 1500 IU/mL rhGM‐CSF (ImmunoTools, Cat# 11343125), and 1500 IU/mL rhIL‐4 (ImmunoTools, Cat# 11340045) and incubated at 37°C and 5% CO 2 for 5 days.

Article Title: The mycotoxin Beauvericin is an uncompetitive inhibitor of Cathepsin B
Article Snippet: On day 3, 4 ml of the medium was exchanged for fresh medium including 1500 IU/ml rhGM-CSF (ImmunoTools, Cat#11343125) and 1500 IU/ml rhIL-4 (ImmunoTools, Cat#11340045).

Article Title: CSF1R + myeloid-monocytic cells drive CAR-T cell resistance in aggressive B cell lymphoma.
Article Snippet: For differentiation of ex vivo generated human CSF1R+CD14+CD68+ LAMM cells we followed a recently established protocol.37 Here we cultivated cells in equal volumes of lymphoma-conditioned media and HAB media (RPMI + 10% human AB-Serum + L-Glutamin + 1% Penicillin/Streptomycin) supplemented with 1 μg/mL of rhIL-4, rhIL-10 and rhM-CSF (all ImmunoTools).

Article Title: Pro-inflammatory macrophages suppress HIV replication in humanized mice and ex vivo co-cultures
Article Snippet: Pro-inflammatory M1 polarization was achieved by adding 20 ng/ml rhIFNγ (Immunotools, Cat#: 11343534) and 100 ng/ml LPS (Sigma Aldrich, Cat#: L2387) for an additional 2 days, and anti-inflammatory M2 polarization by adding 20 ng/ml rhIL-4 (Immunotools, Cat#: 11340043).

Article Title: EFSA Project on the use of NAMs to explore the immunotoxicity of PFAS
Article Snippet: THP-1 cells (1x105 cells/mL) were treated for 5 days with rhIL-4 (1500 UI/mL — ImmunoTools GmbH, Friesoythe, Germany) and rhGM-CSF (1500 UI/mL — ImmunoTools GmbH) to acquire the properties of immature DCs (iDCs) as described by Berges et al. (2005).

Article Title: Pseudopterosin A-D Modulates Dendritic Cell Activation in Skin Sensitization.
Article Snippet: Differentiation was induced by adding 1500 IU/mL rhGM-CSF (ImmunoTools, #11343125, Friesoythe, Germany) and 1500 IU/mL rhIL-4 (ImmunoTools, #11340045), with a medium change performed on day 3.

Cell Culture:

Article Title: The mycotoxin Beauvericin is an uncompetitive inhibitor of Cathepsin B.
Article Snippet: iDCs were generated according to the protocol (Holken and Teusch 2023), namely, 1 106 THP-1 cells were cultured in a T25 flask with 5 mL RPMI (Thermo Fisher Scientific, Cat# 31870-025) including 10% heat-inactivated FCS (Sigma-Aldrich, Cat# F7524), 0.1% 2-mercaptoethanol (Thermo Fischer Scientific, Cat# 31350-010), 1% PenStrep (Biochrom GmbH, Cat# A2212), 1500 IU/mL rhGM-CSF (ImmunoTools, Cat# 11343125), and 1500 IU/mL rhIL-4 (ImmunoTools, Cat# 11340045) and incubated at 37 C and 5% CO2 for 5 days.

Article Title: Pro-inflammatory macrophages suppress HIV replication in humanized mice and ex vivo co-cultures.
Article Snippet: Pro-inflammatory M1 polarization was achieved by adding 20 ng/ml rhIFNg (Immunotools, Cat#: 11343534) and 100 ng/ml LPS (SigmaAldrich, Cat#: L2387) for an additional 2 days, and anti-inflammatory M2 polarization by adding 20 ng/ml rhIL-4 (Immunotools, Cat#: 11340043).

Article Title: The mycotoxin Beauvericin is an uncompetitive inhibitor of Cathepsin B
Article Snippet: iDCs were generated according to the protocol (Holken and Teusch ), namely, 1 × 10 6 THP‐1 cells were cultured in a T25 flask with 5 mL RPMI (Thermo Fisher Scientific, Cat# 31870‐025) including 10% heat‐inactivated FCS (Sigma‐Aldrich, Cat# F7524), 0.1% 2‐mercaptoethanol (Thermo Fischer Scientific, Cat# 31350‐010), 1% PenStrep (Biochrom GmbH, Cat# A2212), 1500 IU/mL rhGM‐CSF (ImmunoTools, Cat# 11343125), and 1500 IU/mL rhIL‐4 (ImmunoTools, Cat# 11340045) and incubated at 37°C and 5% CO 2 for 5 days.

Article Title: The mycotoxin Beauvericin is an uncompetitive inhibitor of Cathepsin B
Article Snippet: On day 3, 4 ml of the medium was exchanged for fresh medium including 1500 IU/ml rhGM-CSF (ImmunoTools, Cat#11343125) and 1500 IU/ml rhIL-4 (ImmunoTools, Cat#11340045).

Article Title: CSF1R + myeloid-monocytic cells drive CAR-T cell resistance in aggressive B cell lymphoma.
Article Snippet: For differentiation of ex vivo generated human CSF1R+CD14+CD68+ LAMM cells we followed a recently established protocol.37 Here we cultivated cells in equal volumes of lymphoma-conditioned media and HAB media (RPMI + 10% human AB-Serum + L-Glutamin + 1% Penicillin/Streptomycin) supplemented with 1 μg/mL of rhIL-4, rhIL-10 and rhM-CSF (all ImmunoTools).

Article Title: Pro-inflammatory macrophages suppress HIV replication in humanized mice and ex vivo co-cultures
Article Snippet: Pro-inflammatory M1 polarization was achieved by adding 20 ng/ml rhIFNγ (Immunotools, Cat#: 11343534) and 100 ng/ml LPS (Sigma Aldrich, Cat#: L2387) for an additional 2 days, and anti-inflammatory M2 polarization by adding 20 ng/ml rhIL-4 (Immunotools, Cat#: 11340043).

Article Title: EFSA Project on the use of NAMs to explore the immunotoxicity of PFAS
Article Snippet: THP-1 cells (1x105 cells/mL) were treated for 5 days with rhIL-4 (1500 UI/mL — ImmunoTools GmbH, Friesoythe, Germany) and rhGM-CSF (1500 UI/mL — ImmunoTools GmbH) to acquire the properties of immature DCs (iDCs) as described by Berges et al. (2005).

Article Title: Pseudopterosin A-D Modulates Dendritic Cell Activation in Skin Sensitization.
Article Snippet: Differentiation was induced by adding 1500 IU/mL rhGM-CSF (ImmunoTools, #11343125, Friesoythe, Germany) and 1500 IU/mL rhIL-4 (ImmunoTools, #11340045), with a medium change performed on day 3.

Incubation:

Article Title: The mycotoxin Beauvericin is an uncompetitive inhibitor of Cathepsin B.
Article Snippet: iDCs were generated according to the protocol (Holken and Teusch 2023), namely, 1 106 THP-1 cells were cultured in a T25 flask with 5 mL RPMI (Thermo Fisher Scientific, Cat# 31870-025) including 10% heat-inactivated FCS (Sigma-Aldrich, Cat# F7524), 0.1% 2-mercaptoethanol (Thermo Fischer Scientific, Cat# 31350-010), 1% PenStrep (Biochrom GmbH, Cat# A2212), 1500 IU/mL rhGM-CSF (ImmunoTools, Cat# 11343125), and 1500 IU/mL rhIL-4 (ImmunoTools, Cat# 11340045) and incubated at 37 C and 5% CO2 for 5 days.

Article Title: Pro-inflammatory macrophages suppress HIV replication in humanized mice and ex vivo co-cultures.
Article Snippet: Pro-inflammatory M1 polarization was achieved by adding 20 ng/ml rhIFNg (Immunotools, Cat#: 11343534) and 100 ng/ml LPS (SigmaAldrich, Cat#: L2387) for an additional 2 days, and anti-inflammatory M2 polarization by adding 20 ng/ml rhIL-4 (Immunotools, Cat#: 11340043).

Article Title: The mycotoxin Beauvericin is an uncompetitive inhibitor of Cathepsin B
Article Snippet: iDCs were generated according to the protocol (Holken and Teusch ), namely, 1 × 10 6 THP‐1 cells were cultured in a T25 flask with 5 mL RPMI (Thermo Fisher Scientific, Cat# 31870‐025) including 10% heat‐inactivated FCS (Sigma‐Aldrich, Cat# F7524), 0.1% 2‐mercaptoethanol (Thermo Fischer Scientific, Cat# 31350‐010), 1% PenStrep (Biochrom GmbH, Cat# A2212), 1500 IU/mL rhGM‐CSF (ImmunoTools, Cat# 11343125), and 1500 IU/mL rhIL‐4 (ImmunoTools, Cat# 11340045) and incubated at 37°C and 5% CO 2 for 5 days.

Article Title: The mycotoxin Beauvericin is an uncompetitive inhibitor of Cathepsin B
Article Snippet: On day 3, 4 ml of the medium was exchanged for fresh medium including 1500 IU/ml rhGM-CSF (ImmunoTools, Cat#11343125) and 1500 IU/ml rhIL-4 (ImmunoTools, Cat#11340045).

Article Title: CSF1R + myeloid-monocytic cells drive CAR-T cell resistance in aggressive B cell lymphoma.
Article Snippet: For differentiation of ex vivo generated human CSF1R+CD14+CD68+ LAMM cells we followed a recently established protocol.37 Here we cultivated cells in equal volumes of lymphoma-conditioned media and HAB media (RPMI + 10% human AB-Serum + L-Glutamin + 1% Penicillin/Streptomycin) supplemented with 1 μg/mL of rhIL-4, rhIL-10 and rhM-CSF (all ImmunoTools).

Article Title: Pro-inflammatory macrophages suppress HIV replication in humanized mice and ex vivo co-cultures
Article Snippet: Pro-inflammatory M1 polarization was achieved by adding 20 ng/ml rhIFNγ (Immunotools, Cat#: 11343534) and 100 ng/ml LPS (Sigma Aldrich, Cat#: L2387) for an additional 2 days, and anti-inflammatory M2 polarization by adding 20 ng/ml rhIL-4 (Immunotools, Cat#: 11340043).

Article Title: EFSA Project on the use of NAMs to explore the immunotoxicity of PFAS
Article Snippet: THP-1 cells (1x105 cells/mL) were treated for 5 days with rhIL-4 (1500 UI/mL — ImmunoTools GmbH, Friesoythe, Germany) and rhGM-CSF (1500 UI/mL — ImmunoTools GmbH) to acquire the properties of immature DCs (iDCs) as described by Berges et al. (2005).

Article Title: Pseudopterosin A-D Modulates Dendritic Cell Activation in Skin Sensitization.
Article Snippet: Differentiation was induced by adding 1500 IU/mL rhGM-CSF (ImmunoTools, #11343125, Friesoythe, Germany) and 1500 IU/mL rhIL-4 (ImmunoTools, #11340045), with a medium change performed on day 3.



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Image Search Results


The cuproptosis blocker SS-31 ameliorated airway responsiveness and airway inflammation in chronic asthma model mice (n = 10 mice per group). ( A ) Ers, Rrs and Rn in different groups challenged with aerosol Mch (mg/ml). ( B ) Representative images of HE-stained lung tissue. C. Cell classification (eosinophils, lymphocytes, and neutrophils) and counts in BALF. D. The levels of the inflammatory factors IL-4, TGFβ1 and IL-1β in the BALF. The data are shown as the means ± SEMs, n = 10. One-way analysis of variance (ANOVA) and the Student–Newman–Keuls (SNK) method were used, and P < 0.05 was considered to indicate statistical significance. *p < 0.05 for the OVA group vs. the control group. # p < 0.05 for the SS-31 + OVA group vs. the PBS + OVA group. Above experiment was repeated three times.

Journal: Scientific Reports

Article Title: Cuproptosis of bronchial epithelial cells triggers airway remodeling in chronic asthma

doi: 10.1038/s41598-025-34574-3

Figure Lengend Snippet: The cuproptosis blocker SS-31 ameliorated airway responsiveness and airway inflammation in chronic asthma model mice (n = 10 mice per group). ( A ) Ers, Rrs and Rn in different groups challenged with aerosol Mch (mg/ml). ( B ) Representative images of HE-stained lung tissue. C. Cell classification (eosinophils, lymphocytes, and neutrophils) and counts in BALF. D. The levels of the inflammatory factors IL-4, TGFβ1 and IL-1β in the BALF. The data are shown as the means ± SEMs, n = 10. One-way analysis of variance (ANOVA) and the Student–Newman–Keuls (SNK) method were used, and P < 0.05 was considered to indicate statistical significance. *p < 0.05 for the OVA group vs. the control group. # p < 0.05 for the SS-31 + OVA group vs. the PBS + OVA group. Above experiment was repeated three times.

Article Snippet: When the cells reached 60–80% confluence, the BEAS-2B cells were incubated with recombinant human IL-4 (rhIL-4) and TGF-β1 (10 ng/mL, R&D Systems) for 24 h to stimulate airway remodeling in vitro.

Techniques: Aerosol, Staining, Control

Cuproptosis was increased in BEAS-2B cells incubated with rhIL-4 and TGFβ1 and the addition of SS-31 reversed the above process. ( A ) Quantification of the effects of different concentrations of SS-31 on the cell viability of BEAS-2B cells. ( B ) Cu 2+ levels in the supernatant of BEAS-2B cells. ( C ) Cu 2+ levels in the cytoplasm of BEAS-2B cells. D. Protein expression of cuproptosis-related genes (FDX-1, LIAS, and DLAT) in BEAS-2B cells. ( D ) Quantification of the protein expression levels of cuproptosis-related genes in BEAS-2B cells. The data are shown as the means ± SEMs. *p < 0.05 for the rhIL-4 + TGFβ1 PBS group vs. the rhIL-4 + TGFβ1 group. # p < 0.05 for the SS-31 + rhIL-4 + TGFβ1 group vs. the PBS + rhIL-4 + TGFβ1 group. Above experiment was repeated fourth times.

Journal: Scientific Reports

Article Title: Cuproptosis of bronchial epithelial cells triggers airway remodeling in chronic asthma

doi: 10.1038/s41598-025-34574-3

Figure Lengend Snippet: Cuproptosis was increased in BEAS-2B cells incubated with rhIL-4 and TGFβ1 and the addition of SS-31 reversed the above process. ( A ) Quantification of the effects of different concentrations of SS-31 on the cell viability of BEAS-2B cells. ( B ) Cu 2+ levels in the supernatant of BEAS-2B cells. ( C ) Cu 2+ levels in the cytoplasm of BEAS-2B cells. D. Protein expression of cuproptosis-related genes (FDX-1, LIAS, and DLAT) in BEAS-2B cells. ( D ) Quantification of the protein expression levels of cuproptosis-related genes in BEAS-2B cells. The data are shown as the means ± SEMs. *p < 0.05 for the rhIL-4 + TGFβ1 PBS group vs. the rhIL-4 + TGFβ1 group. # p < 0.05 for the SS-31 + rhIL-4 + TGFβ1 group vs. the PBS + rhIL-4 + TGFβ1 group. Above experiment was repeated fourth times.

Article Snippet: When the cells reached 60–80% confluence, the BEAS-2B cells were incubated with recombinant human IL-4 (rhIL-4) and TGF-β1 (10 ng/mL, R&D Systems) for 24 h to stimulate airway remodeling in vitro.

Techniques: Incubation, Expressing

Silencing FDX-1 attenuated cell remodeling in BEAS-2B cells incubated with rhIL-4 and TGFβ1. ( A , B ) Quantification of FDX-1 protein expression in BEAS-2B cells transfected with different siRNAs. ( C ) Cell morphology in different groups was observed under a light microscope. ( D , E ) Quantification of the effect of FDX-1 knockdown on cell migratory function. F. Effects of FDX-1 knockdown on the protein expression of EMT-related MMP-2, MMP-9, E-cadherin, N-cadherin, vimentin and smooth muscle-related α-SMA in BEAS-2B cells. The data are shown as the means ± SEMs. *p < 0.05 for the rhIL-4 + TGFβ1 PBS group vs. the rhIL-4 + TGFβ1 group. # p < 0.05 for the Scr + rhIL-4 + TGFβ1 group vs. the Si + rhIL-4 + TGFβ1 group. Above experiment was repeated fourth times.

Journal: Scientific Reports

Article Title: Cuproptosis of bronchial epithelial cells triggers airway remodeling in chronic asthma

doi: 10.1038/s41598-025-34574-3

Figure Lengend Snippet: Silencing FDX-1 attenuated cell remodeling in BEAS-2B cells incubated with rhIL-4 and TGFβ1. ( A , B ) Quantification of FDX-1 protein expression in BEAS-2B cells transfected with different siRNAs. ( C ) Cell morphology in different groups was observed under a light microscope. ( D , E ) Quantification of the effect of FDX-1 knockdown on cell migratory function. F. Effects of FDX-1 knockdown on the protein expression of EMT-related MMP-2, MMP-9, E-cadherin, N-cadherin, vimentin and smooth muscle-related α-SMA in BEAS-2B cells. The data are shown as the means ± SEMs. *p < 0.05 for the rhIL-4 + TGFβ1 PBS group vs. the rhIL-4 + TGFβ1 group. # p < 0.05 for the Scr + rhIL-4 + TGFβ1 group vs. the Si + rhIL-4 + TGFβ1 group. Above experiment was repeated fourth times.

Article Snippet: When the cells reached 60–80% confluence, the BEAS-2B cells were incubated with recombinant human IL-4 (rhIL-4) and TGF-β1 (10 ng/mL, R&D Systems) for 24 h to stimulate airway remodeling in vitro.

Techniques: Incubation, Expressing, Transfection, Light Microscopy, Knockdown