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pp1a (MedChemExpress)

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MedChemExpress pp1a
Pp1a, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pp1a/SARS-CoV-2+PLpro/pm41444683-68-24-46
Average 94 stars, based on 3 article reviews

pp1a - by Bioz Stars, 2026-09

94/100 stars

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Article Title: 4R-tau isoform induction via TDP-43 in neurons in response to insulin: converging signaling pathways with implications for neurodegenerative disease.
Article Snippet: .. ; mouse NGF (nerve growth factor 2.5 S Alomone N-100 ) at 100 ng/ml for 60 min as in [20]; Okadaic Acid (OA) as PP1a and PP2a 10 nM and 100 nM 24 h before incubation with insulin as used in [21] ; CK1 inhibitor D4776 (Medchemexpress, stock 1 mg/25 ml DMSO) 100 μM and 1000 μM 1 h before other treatment was used at the following concentrations before incubation with insulin as used in [22]; CK2 inhibitor DMAT (Medchemexpress, HY-15535, (stock 1 mg/ DMSO) 20 μM and 200 μM, 24 h incubation before other treatment, as previously used in [23]. .. For whole-cell lysate (WCL) preparation, cells were washed once in PBS and resuspended in a 1% SDS HEPES pH7.4 lysis buffer containing a protease inhibitor cocktail (Roche, cOmpleteTM) with or without the addition of phosphatase inhibitors: NaF (10mM) and Na3VO4 (2mM), as stated in the text, and sonicated (Fisher Scientific Sonic Dismembrator, model 500) at a 20% amplitude for a 15 s pulse.

Article Title: 4R-tau isoform induction via TDP-43 in neurons in response to insulin: converging signaling pathways with implications for neurodegenerative disease
Article Snippet: .. ; mouse NGF (nerve growth factor 2.5 S Alomone N-100 ) at 100 ng/ml for 60 min as in [ ]; Okadaic Acid ( OA ) as PP1a and PP2a 10 nM and 100 nM 24 h before incubation with insulin as used in [ ] ; CK1 inhibitor D4776 (Medchemexpress, stock 1 mg/25 ml DMSO) 100 μM and 1000 μM 1 h before other treatment was used at the following concentrations before incubation with insulin as used in [ ]; CK2 inhibitor DMAT (Medchemexpress, HY-15535, (stock 1 mg/DMSO) 20 μM and 200 μM, 24 h incubation before other treatment, as previously used in [ ]. .. For whole-cell lysate (WCL) preparation, cells were washed once in PBS and resuspended in a 1% SDS HEPES pH7.4 lysis buffer containing a protease inhibitor cocktail (Roche, cOmpleteTM) with or without the addition of phosphatase inhibitors: NaF (10mM) and Na 3 VO 4 (2mM), as stated in the text, and sonicated (Fisher Scientific Sonic Dismembrator, model 500) at a 20% amplitude for a 15 s pulse.

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pp1a (MedChemExpress)

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