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phsg396 plasmid  (ATCC)


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    Structured Review

    ATCC phsg396 plasmid
    Phsg396 Plasmid, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20065 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phsg396/Plasmid/pmc11792519-106-32-49
    Average 99 stars, based on 20065 article reviews
    phsg396 plasmid - by Bioz Stars, 2026-09
    99/100 stars

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    other:

    Article Title: In vivo interspecies dissemination of IncM2-type bla NDM-1 carrying plasmid.
    Article Snippet: Month XXXX Volume 0 Issue 0 10.1128/spectrum.00399-24 3 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 25 D ec em be r 20 24 b y 24 05 :a cc 0: 12 07 :d 43 :2 88 b: 75 82 :6 4f :1 bf 1. from E. coli isolate 4974960 into K. pneumoniae ATCC 700603 with pHSG396 was 6.4 × 10−5.



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    Growth curves. C. sedlakii 4972101, recombinant E. coli- SED-1 and E. coli- SED-2, and E. coli DH10β carrying an empty pHSG396 plasmid (negative control) were grown in BHI broth with and without: ( A ) ceftazidime (CAZ) (16 µg/mL), as indicated. ( B ) Cefotaxime (FOX) (16 µg/mL). Bars represent standard error.

    Journal: Microbiology Spectrum

    Article Title: A single amino acid substitution in SED-2 β-lactamase leads to increased inhibitor resistance and an extended substrate spectrum

    doi: 10.1128/spectrum.00224-25

    Figure Lengend Snippet: Growth curves. C. sedlakii 4972101, recombinant E. coli- SED-1 and E. coli- SED-2, and E. coli DH10β carrying an empty pHSG396 plasmid (negative control) were grown in BHI broth with and without: ( A ) ceftazidime (CAZ) (16 µg/mL), as indicated. ( B ) Cefotaxime (FOX) (16 µg/mL). Bars represent standard error.

    Article Snippet: The resulting PCR fragment was restricted by XbaI and EcoRI (New England Biolabs, Ipswich, MA, USA) and ligated into a pHSG396 plasmid with T4 DNA ligase (New England Biolabs).

    Techniques: Recombinant, Plasmid Preparation, Negative Control