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Growth curves. C. sedlakii 4972101, recombinant E. coli- SED-1 and E. coli- SED-2, and E. coli DH10β carrying an empty <t>pHSG396</t> plasmid (negative control) were grown in BHI broth with and without: ( A ) ceftazidime (CAZ) (16 µg/mL), as indicated. ( B ) Cefotaxime (FOX) (16 µg/mL). Bars represent standard error.
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Growth curves. C. sedlakii 4972101, recombinant E. coli- SED-1 and E. coli- SED-2, and E. coli DH10β carrying an empty <t>pHSG396</t> plasmid (negative control) were grown in BHI broth with and without: ( A ) ceftazidime (CAZ) (16 µg/mL), as indicated. ( B ) Cefotaxime (FOX) (16 µg/mL). Bars represent standard error.
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Growth curves. C. sedlakii 4972101, recombinant E. coli- SED-1 and E. coli- SED-2, and E. coli DH10β carrying an empty <t>pHSG396</t> plasmid (negative control) were grown in BHI broth with and without: ( A ) ceftazidime (CAZ) (16 µg/mL), as indicated. ( B ) Cefotaxime (FOX) (16 µg/mL). Bars represent standard error.
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Growth curves. C. sedlakii 4972101, recombinant E. coli- SED-1 and E. coli- SED-2, and E. coli DH10β carrying an empty <t>pHSG396</t> plasmid (negative control) were grown in BHI broth with and without: ( A ) ceftazidime (CAZ) (16 µg/mL), as indicated. ( B ) Cefotaxime (FOX) (16 µg/mL). Bars represent standard error.
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Growth curves. C. sedlakii 4972101, recombinant E. coli- SED-1 and E. coli- SED-2, and E. coli DH10β carrying an empty pHSG396 plasmid (negative control) were grown in BHI broth with and without: ( A ) ceftazidime (CAZ) (16 µg/mL), as indicated. ( B ) Cefotaxime (FOX) (16 µg/mL). Bars represent standard error.

Journal: Microbiology Spectrum

Article Title: A single amino acid substitution in SED-2 β-lactamase leads to increased inhibitor resistance and an extended substrate spectrum

doi: 10.1128/spectrum.00224-25

Figure Lengend Snippet: Growth curves. C. sedlakii 4972101, recombinant E. coli- SED-1 and E. coli- SED-2, and E. coli DH10β carrying an empty pHSG396 plasmid (negative control) were grown in BHI broth with and without: ( A ) ceftazidime (CAZ) (16 µg/mL), as indicated. ( B ) Cefotaxime (FOX) (16 µg/mL). Bars represent standard error.

Article Snippet: The resulting PCR fragment was restricted by XbaI and EcoRI (New England Biolabs, Ipswich, MA, USA) and ligated into a pHSG396 plasmid with T4 DNA ligase (New England Biolabs).

Techniques: Recombinant, Plasmid Preparation, Negative Control