Review




Structured Review

Promega basic pcat vector
Basic Pcat Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat-basic/pcat+basic/pmc11193803-334-39-41
Average 90 stars, based on 1 article reviews
basic pcat vector - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Clone Assay:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Plasmid Preparation:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Negative Control:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Sequencing:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Positive Control:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Expressing:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Activity Assay:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Standard Deviation:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Transfection:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Luciferase:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Reporter Assay:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Construct:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Amplification:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Polymerase Chain Reaction:

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: Construction of α2(V)-CAT fusion plasmids The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).

Article Title: Impact of mutant β -catenin on ABCB1 expression and therapy response in colon cancer cells
Article Snippet: For CAT-ELISA, the promoter-less plasmid pCAT-Basic (Promega, Mannheim, Germany) and transfections without DNA served as controls.

Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
Article Snippet: CAT activity, as corroboration for the luciferase reporter assay, was measured using pCAT-basic, pCAT-control, and experimental constructs in cells harvested 48 hours after transfection (Promega).

Article Title: A Selective Role for MRF4 in Innervated Adult Skeletal Muscle: Na V 1.4 Na + Channel Expression Is Reduced in MRF4-Null Mice
Article Snippet: As a negative control, the reporter gene without an upstream sequence was used (pCAT-Basic, Promega), and as a positive control, the reporter gene driven by the SV40 promoter and enhancer was used (pCAT-Control, Promega).

Article Title: Cellular Transcription Factors Induced in Trigeminal Ganglia during Dexamethasone-Induced Reactivation from Latency Stimulate Bovine Herpesvirus 1 Productive Infection and Certain Viral Promoters
Article Snippet: The numbers in the plasmid name refer to the length of the bICP0 E promoter fragment cloned into the promoterless vector, pCAT-basic (Promega).

Article Title: Nuclear Factor YY1 Activates the Mammalian F 0 F 1 ATP Synthase α -Subunit Gene
Article Snippet: The +25 to +136 bp or the +68 to +86 bp region of the ATPA gene was cloned into the vector, pCAT-Basic (Promega).

Article Title: Binding of RBP-J? (CSL) Protein to the Promoter of the Kaposi's Sarcoma-Associated Herpesvirus ORF47 (gL) Gene Is a Critical but Not Sufficient Determinant of Trans-activation by ORF50 Protein
Article Snippet: Plasmid construction For construction of CAT reporter plasmids, DNA fragments of the KSHV ORF47 promoter were amplified by PCR from pORF50p(−3870/+20)/CAT ( Chang et al., 2005 ) and cloned into pCAT-Basic (Promega) digested with HindIII and PstI.

Article Title: Homology between α2(V) and αl(lll) collagen promoters and evidence for negatively acting elements in the α2(V) first intron and 5′ flanking sequences
Article Snippet: The pUC19-derived vectors pCAT-Basic (Promega, technical bulletin #80) and pCAT-Enhancer (Promega, technical bulletin #82) contain the bacterial chloramphenicol acetyltransferase (CAT) gene in a 773-bp TaqI fragment ( Gorman et al., 1982 ).



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Image Search Results


The  CAT  expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of  CAT  reporter plasmids

Journal: Virology Journal

Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses

doi: 10.1186/1743-422X-6-212

Figure Lengend Snippet: The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

Article Snippet: Lipofectamine™ was purchased from Invitrogen (Beijing, China); plasmid purification Mini Kit was from Qiagen (Shanghai, China); pCAT-Basic vector was from Promega (Beijing, China); CAT ELISA detection Kit was from Roche (Shanghai, China); SPF chicken embryos were from SFAFAS Company (Jinan, China).

Techniques: Expressing, Transfection

Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

Journal: Gene Expression

Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs

doi:

Figure Lengend Snippet: Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

Article Snippet: All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) ( 12 ).

Techniques: Transfection, Activity Assay, Construct, Clone Assay, Expressing, Negative Control