pcat basic (Promega)
Structured Review

Pcat Basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat-basic/pcat+basic/pmc06157365-233-10-12
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs"
Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs
Journal: Gene Expression
doi:
Figure Legend Snippet: Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.
Techniques Used: Transfection, Activity Assay, Construct, Clone Assay, Expressing, Negative Control
Related Articles
other:Article Title: Cell-Specific Transcription of the Smooth Muscle γ-Actin Gene Requires Both Positive and Negative-Acting cis Elements Article Snippet: To facilitate the subcloning of truncated 5′ genomic sequences into the polylinker region of the Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses Article Snippet: LipofectamineTM was purchased from Invitrogen (Beijing, China); plasmid purification Mini Kit was from Qiagen (Shanghai, China); pCAT-Basic vector was from Promega (Beijing, China); CAT ELISA detection Kit was from Roche (Shanghai, China); SPF chicken embryos were from SFAFAS Company (Jinan, China). Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses Article Snippet: The divided promoters were amplified with only the template of GA. All the PCR fragments were sequenced before inserted into the Kpn I/ Sac I sites of Polymerase Chain Reaction:Article Title: DNA methylation as an epigenetic mechanism in the regulation of LEDGF expression and biological response in aging and oxidative stress Article Snippet: .. The genomic P1 clone comprising the LEDGF gene was subjected to Genomic PCR with primers containing Mlu I and Nhe I, and fragments encompassing −315 to +35, −170 to +35, and −63 to +35 bps were ligated into basic Plasmid Preparation:Article Title: Hydralazine Revives Cellular and Ocular Lens Health-Span by Ameliorating the Aging and Oxidative-Dependent Loss of the Nrf2-Activated Cellular Stress Response Article Snippet: .. To engineer pCAT-linked hPrdx6 promoter plasmid, the DNA fragment (−918 to +30 bp) was ligated into basic Article Title: Neuroprotective effect of peroxiredoxin 6 against hypoxia-induced retinal ganglion cell damage Article Snippet: .. A construct of -1139 bp was prepared by ligating it to basic Article Title: Hydralazine Revives Cellular and Ocular Lens Health-Span by Ameliorating the Aging and Oxidative-Dependent Loss of the Nrf2-Activated Cellular Stress Response. Article Snippet: .. To engineer pCAT-linked hPrdx6 promoter plasmid, the DNA fragment (−918 to +30 bp) was ligated into basic Construct:Article Title: Neuroprotective effect of peroxiredoxin 6 against hypoxia-induced retinal ganglion cell damage Article Snippet: .. A construct of -1139 bp was prepared by ligating it to basic Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs Article Snippet: .. All intron constructs carry the HcTnC4500 full promoter cloned into Clone Assay:Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs Article Snippet: .. All intron constructs carry the HcTnC4500 full promoter cloned into |
