pc12 cells  (ATCC)

Journal: Neural Regeneration Research

Article Title: Polydopamine-coupled NT 3 -derived oriented conductive scaffolds with immunomodulatory properties accelerate peripheral nerve regeneration

doi: 10.4103/NRR.NRR-D-24-01544

Figure Lengend Snippet: Effect of PLGA/CNT-PDA-NT 3 on PC12 cell growth. (A) Schematic diagram of the mechanism through which neural conduits promote PC12 cell axon growth. (B) CLSM images of PC12 cells. DAPI staining is shown in blue, and phalloidin staining is shown in green (scale bar: 200 μm). (C) Average PC12 cell axon length ( n = 6). (D) Quantitative analysis of immunofluorescence intensity ( n = 6). (E–G) Polarity histogram of the directional distribution of PC12 cell axons on scaffolds. (H–J) Relative gene expression levels of neuronal markers ( NF200 , GAP43 , and Tubb3 ), as determined by quantitative reverse transcription-polymerase chain reaction. Data are expressed as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, vs . PLGA/CNT; # P < 0.05, ## P < 0.01, vs . PLGA/CNT-PDA (one-way analysis of variance followed by Tukey’s post hoc test). CLSM: Confocal laser scanning microscopy; CNT: carbon nanotube; GAP43: growth associated protein 43; NF200: neurofilament-200; NT 3 : neurotrophin-3; PDA: polydopamine; PLGA: polylactic-glycolic acid.

Article Snippet: RSC96, PC12, and RAW 264.7 (CL-0190, Procell Life Science & Technology Co., Ltd, Wuhan, China) cells were seeded into a 96-well plate at a density of 8 × 10 3 cells per well.

Techniques: Staining, Immunofluorescence, Gene Expression, Reverse Transcription, Polymerase Chain Reaction, Confocal Laser Scanning Microscopy

Journal: Neural Regeneration Research

Article Title: Polydopamine-coupled NT 3 -derived oriented conductive scaffolds with immunomodulatory properties accelerate peripheral nerve regeneration

doi: 10.4103/NRR.NRR-D-24-01544

Figure Lengend Snippet: Antioxidant and immunomodulatory effects of PLGA/CNT-PDA-NT 3 . (A) Schematic diagram of ROS scavenging and macrophage phenotype induction by the fibrous scaffolds. (B) DPPH test of antioxidant capacity ( n = 3). (C) ROS probe test (scale bar: 200 μm). (D) Quantitative analysis of ROS fluorescence intensity ( n = 3). (E–G) Survival rates of RSC96, PC12, and RAW264.7 cells exposed to 100 μM H 2 O 2 , as measured by Cell Counting Kit-8 ( n = 3). (H) Fluorescence images showing the cytoskeletons and nuclei of RAW 264.7 macrophages cultured for 24 hours under normal conditions or on fibrous scaffolds. Arrows indicate cells with M2 morphology (scale bar: 50 μm). (I–L) TNF-α , iNOS , IL-6 , IL-10 , and mRNA expression in RAW264.7 cells, as determined by quantitative reverse transcription-polymerase chain reaction. Data are expressed as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, vs . PLGA/CNT; # P < 0.05, ## P < 0.01, vs . PLGA/CNT-PDA; $$ P < 0.01, vs . control /H 2 O 2 group (one-way analysis of variance followed by Tukey’s post hoc test). CNT: Carbon nanotube; IL: interleukin; iNOS: inducible nitric oxide synthase; NT 3 : neurotrophin-3; PDA: polydopamine; PLGA: polylactic-glycolic acid; ROS: reactive oxygen species; TNF-α: tumor necrosis factor-α.

Article Snippet: RSC96, PC12, and RAW 264.7 (CL-0190, Procell Life Science & Technology Co., Ltd, Wuhan, China) cells were seeded into a 96-well plate at a density of 8 × 10 3 cells per well.

Techniques: Fluorescence, Cell Counting, Cell Culture, Expressing, Reverse Transcription, Polymerase Chain Reaction, Control