ovalbumin  (InvivoGen)

Journal: The Journal of Immunology Author Choice

Article Title: Sorting nexin 5 deficiency impairs MHC class II antigen presentation and inflammatory responses during tuberculosis

doi: 10.1093/jimmun/vkag217

Figure Lengend Snippet: Snx5 deficiency impairs antigen presentation by macrophages and DCs. (A) Timeline of antigen presentation assay. (B, C) BMDMs, (D) RAW 264.7, or (E, F) DCs, either primed or not with IFN-γ, were fed with OVA 323–339 , Mtb Ag85B, or heat-killed Mtb as depicted on the graphs. After (D) 4 h or (B, C, E, and F) 18 h, the cells were incubated with antigen-specific MHC class II–restricted T cell hybridomas. After 24 h, supernatants were assessed for IL-2 secretion by T cells. Bars are the mean ± SD from 1 of 3 experiments performed at least in triplicate. The circles shown correspond to each replica. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (for indicated comparison; t test).

Article Snippet: After overnight incubation, the cells were fed with vehicle or ovalbumin 323–339 (OVA 323–339 ) (the epitope of OVA presented by I-Ab, 5 mg/mL) (InvivoGen; 5825-43-01), Ag85B (10 ng/mL) (Ray-Biotech, 228-11030 (discontinued) or BEI, NR5326), or heat-killed Mtb (MOI of 10) ( ).

Techniques: Immunopeptidomics, Incubation, Comparison