Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: The summary results inflammation cytokines level by ELISA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: B. infantis reduced the expression of IgE in the serum of allergic asthma mice. ( A ) ELISA was used to measure the total IgE content (ng/ml) in the serum of mice. ( B ) ELISA was used to detect the content of OVA-specific IgE (ng/ml) in the serum of mice. All experiments were repeated in triplicate to average (n=10). ## p <0.001 vs. control; ** p <0.001 vs. OVA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: B. infantis regulated the balance of Th1/Th2-related cytokines in BALF and lung tissues. ( A–E ) ELISA was used to detect the contents of IFN-γ, IL-2, IL-4, IL-5, and IL-13 in BALF. ( F–J ) QRT-PCR was used to detect the expression of IFN-γ, IL-2, IL-4, IL-5, and IL-13 in lung tissues. β-actin served as a reference gene. All experiments were repeated in triplicate to average (n=10). # p <0.05, ## p <0.001 vs. control; * p <0.05, ** p <0.001 vs. OVA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing, Control

Journal: Pharmaceutics

Article Title: The Potential of Cell-Penetrating Peptides for mRNA Delivery to Cancer Cells

doi: 10.3390/pharmaceutics14061271

Figure Lengend Snippet: Evaluation of CPP/mRNA-mediated protein expression. ( A ) Representative fluorescence images and a quantitative graph showing mCherry-positive CT26.CL25 cells 24 h after treatment with CPP/mCherry mRNA complexes (6.8 nM). Scale bar: 275 μm. Data are presented as the mean ± SD ( n = 3). ( B ) Representative flow cytometry histograms and graphs of EGFP-positive CL26.CL25 cells transfected with CPP/EGFP mRNA complexes (6.8 nM). Data are presented as the mean ± SD ( n = 3). ( C ) Western blot analysis of the expression levels of OVA and GAPDH in CT26.CL25 cells.

Article Snippet: The following western blot antibodies were used: OVA (Bio-Rad, #0220-1682, 1:1000), GAPDH (R&D, MAB5718, 1:1000), Anti-Rabbit IgG-HRP antibody (Gene Tex, GTX213110-01, 1:2000) and Anti-mouse IgG-HRP antibody (GebeTex, GTX213111-01, 1:2000).

Techniques: Expressing, Fluorescence, Flow Cytometry, Transfection, Western Blot

Journal: PloS one

Article Title: Cis-acting pathways selectively enforce the non-immunogenicity of shed placental antigen for maternal CD8 T cells.

doi: 10.1371/journal.pone.0084064

Figure Lengend Snippet: Figure 1. Shedding of mOVA into maternal blood. OVA immunoprecipitation followed by anti-OVA western blotting was performed on plasma samples. For comparison, 10 and 50 ng of sOVA were directly loaded into the last two lanes of each gel. (A) Plasma from B6- and Act-mOVA-mated pregnant mice was pooled (n = 4 mice per pool) and purified by differential centrifugation. The amount of material loaded into each lane was derived from the same initial amount of plasma (25 ml). (B) sOVA clearance from mouse plasma. Mice were uninjected (-) or injected with 100 mg sOVA. Plasma was obtained at the indicated times post-injection. Both blots are representative of two independent experiments. doi:10.1371/journal.pone.0084064.g001

Article Snippet: 50 ml of the indicated plasma fraction, or the corresponding amount of pelleted material, was then incubated with rabbit anti-OVA Abs (Fitzgerald Industries International) followed by dynabead-coupled sheep anti-rabbit Ig (Invitrogen) overnight at 4uC in a total volume of 0.8–1.0 ml.

Techniques: Immunoprecipitation, Western Blot, Clinical Proteomics, Comparison, Purification, Centrifugation, Derivative Assay, Injection