Journal: Nucleic Acids Research
Article Title: LSD1 controls a nuclear checkpoint in Wnt/β-Catenin signaling to regulate muscle stem cell self-renewal
doi: 10.1093/nar/gkae060
Figure Lengend Snippet: LSD1 regulates MuSCs self-renewal potential. ( A ) PAX7 and MyHC staining and percentage of PAX7+ cells after 48 h under myogenic differentiation conditions of CTRL SC and LSD1 SCiKO MuSCs. ( B ) PAX7 and MyHC staining and percentage of PAX7+ cells after 48 h under myogenic differentiation conditions of MuSCs treated with LSD1 inhibitors (OG-L002 and Pargyline). ( C ) Bodipy, PAX7, desmin and Oil Red O staining of cells after 48 h under myogenic differentiation conditions of CTRL SC and LSD1 SCiKO MuSCs. ( D ) CTX experimental setup. ( E ) Anti-Laminin staining on cryosections of regenerated TA muscles in CTRL SC and LSD1 SCiKO mice at 28 dpi. Quantification of the number of myofibers per mm2. ( F ) CSA distribution of muscle fibers in CTRL SC and LSD1 SCiKO mice TA cryosections at 28 dpi. ( G ) Anti-PAX7 and anti-Laminin staining on cryosections of regenerated TA muscles in CTRL SC and LSD1 SCiKO mice at 28 dpi. Quantification of the number of sublaminar PAX7+/Ki67- cells per mm2. ( H ) CTX experimental setup. ( I ) Anti-PAX7 and anti-Laminin staining on cryosections of regenerated TA muscles in Vehicle and OG-L002 treated mice at 21 dpi. Quantification of the number of sublaminar PAX7+/Ki67− cells per mm 2 . ( J ) H&E and Oil Red O staining on cryosections of regenerated TA muscles in Vehicle and OG-L002 treated mice at 21 dpi. ( K ) Anti-Laminin staining on cryosections of regenerated TA muscles in Vehicle and OG-L002 treated mice at 21 dpi. Quantification of the number of myofibers per mm 2 . Scale bars, 50 μm. n = 3 mice/genotype. n = 3 primary MuSC cultures/genotype. n = 3 primary MuSC cultures/treatments. Values are mean or percentage mean ± SEM. ** P < 0.01 (Mann–Whitney–Wilcoxon test) *** P < 0.001 (Bonferroni test after one way-ANOVA).
Article Snippet: Nine-week-old C57BL/6J mice were purchased from Charles River laboratories and intraperitoneal injected with OG-L002 20 mg/kg or NaCl 0,9% for 7 days.
Techniques: Staining, Muscles, MANN-WHITNEY
