oc2 plasmid lenti orf  (OriGene)

Journal: Molecular medicine (Cambridge, Mass.)

Article Title: ONECUT2 reprograms neuroendocrine fate and is an actionable therapeutic target in small cell lung cancer.

doi: 10.1186/s10020-025-01267-6

Figure Lengend Snippet: Fig. 2 OC2 modulates plasticity from NE to non-NE phenotypes in the DMS53 cell line. A Heatmap showing DEGs (adjusted P-value < 0.001) after OC2 enforced expression in DMS53 cells analyzed by hierarchical clustering. Three independent RNA-Seq experiments were performed per condition. B Plot showing upregulated (red bars) and downregulated (blue bars) SCLC subtype-specific gene signatures (Ireland et al. 2020) after OC2 enforced expres sion. C Immunohistochemical staining of SYP in OC2 overexpressing DMS53 cells. The boxes show the 25–75th percentile range, and the center line is the median. Whiskers extend from the minimum and maximum values. P-values were obtained from Wilcoxon two-tailed rank-sum test. D, E mRNA (D) and protein levels (E) of OC2, ASCL1, NEUROD1 and YAP1 after OC2 enforced expression in DMS53 cells. For (D) qRT-PCR results were normalized using β-actin. The mean + SEM from three independent experiments is shown. Unpaired two-tailed Student’s t-test, *P < 0.05, **P < 0.01. For (E) representative blots from three independent experiments are shown. F Plot showing upregulated (red bars) non-NE gene signatures and downregulated (blue bars) NE gene signatures (Cai et al. 2021; Zhang et al. 2018)

Article Snippet: The empty vector pLenti-C-mGFP-P2A-Puro (Origene, PS100093) was used as a control and the OC2 plasmid Lenti-ORF clone of ONECUT2 (mGFP-tagged)-Human one cut homeobox 2 (Origene, RC211951L4) was used for constitutive expression of this protein.

Techniques: Expressing, RNA Sequencing, Immunohistochemical staining, Staining, Two Tailed Test, Quantitative RT-PCR