Journal: Cell reports

Article Title: Embryonic temporal–spatial delineation of excitatory spinal V3 interneuron diversity

doi: 10.1016/j.celrep.2023.113635

Figure Lengend Snippet: Key resources table

Article Snippet: Human ONECUT2/OC-2 Affinity Purified Polyclonal Ab , R and D Systems , RRID:AB_10640365.

Techniques: Recombinant, Affinity Purification, Produced, Virus, Plasmid Preparation, Labeling, Software

Journal: Molecular medicine (Cambridge, Mass.)

Article Title: ONECUT2 reprograms neuroendocrine fate and is an actionable therapeutic target in small cell lung cancer.

doi: 10.1186/s10020-025-01267-6

Figure Lengend Snippet: Fig. 2 OC2 modulates plasticity from NE to non-NE phenotypes in the DMS53 cell line. A Heatmap showing DEGs (adjusted P-value < 0.001) after OC2 enforced expression in DMS53 cells analyzed by hierarchical clustering. Three independent RNA-Seq experiments were performed per condition. B Plot showing upregulated (red bars) and downregulated (blue bars) SCLC subtype-specific gene signatures (Ireland et al. 2020) after OC2 enforced expres sion. C Immunohistochemical staining of SYP in OC2 overexpressing DMS53 cells. The boxes show the 25–75th percentile range, and the center line is the median. Whiskers extend from the minimum and maximum values. P-values were obtained from Wilcoxon two-tailed rank-sum test. D, E mRNA (D) and protein levels (E) of OC2, ASCL1, NEUROD1 and YAP1 after OC2 enforced expression in DMS53 cells. For (D) qRT-PCR results were normalized using β-actin. The mean + SEM from three independent experiments is shown. Unpaired two-tailed Student’s t-test, *P < 0.05, **P < 0.01. For (E) representative blots from three independent experiments are shown. F Plot showing upregulated (red bars) non-NE gene signatures and downregulated (blue bars) NE gene signatures (Cai et al. 2021; Zhang et al. 2018)

Article Snippet: The empty vector pLenti-C-mGFP-P2A-Puro (Origene, PS100093) was used as a control and the OC2 plasmid Lenti-ORF clone of ONECUT2 (mGFP-tagged)-Human one cut homeobox 2 (Origene, RC211951L4) was used for constitutive expression of this protein.

Techniques: Expressing, RNA Sequencing, Immunohistochemical staining, Staining, Two Tailed Test, Quantitative RT-PCR

Journal: Biomacromolecules

Article Title: Controlling the Nematic Liquid Crystallinity of Cellulose Nanocrystals with an Alcohol Ethoxy Sulfonate Surfactant

doi: 10.1021/acs.biomac.3c01375

Figure Lengend Snippet: (a) Chemical structures of the cellulose nanocrystals (CNCs), the nonionic–anionic trifunctional oligo(ethylene glycol) alkyl-3-sulfopropyl diether potassium salt (AES) surfactant, and the nonionic difunctional reference surfactant ethoxylated alcohol (AE) without the anionic end group. (b) Schematics for CNC/AES processing into lyotropic suspensions. (c) For the (solvent-free) thermotropic CNC/AES system, CNCs were first mixed with an excess of AES surfactant in water, followed by water removal by freeze-drying. No additional organic solvents were used in this case.

Article Snippet: The trifunctional nonionic–anionic surfactant (oligo(ethylene glycol) alkyl-3-sulfopropyl diether potassium salt, i.e., C m H 2 m +1 -(OC 2 H 4 ) n -C 3 H 6 –SO 3 – K + , subsequently denoted as AES) was acquired from Raschig GmbH (type F11–13) ( Figure a).

Techniques: Solvent

Journal: Biomacromolecules

Article Title: Controlling the Nematic Liquid Crystallinity of Cellulose Nanocrystals with an Alcohol Ethoxy Sulfonate Surfactant

doi: 10.1021/acs.biomac.3c01375

Figure Lengend Snippet: (a) FTIR spectra of pristine CNC, AES, and CNC/AES “bound” complex and zoomed in detail for the (b) OH stretching vibration of hydroxyl groups present onto the CNC surface and the (c) distortion of the three AES C–O–C stretching vibration IR bands (in black) in the complex spectra (in blue). (d) Proposed H-bonding interaction between CNCs and the OEG block of AES in the formed complex in toluene, thus providing high charge crowding on CNCs. QCM-D results of AES (e) and AE (nonionic reference surfactant) (f) adsorption on the CNC thin film surface. AES binds to the CNC surface according to Δ f −11 Hz frequency change after rinsing with toluene.

Article Snippet: The trifunctional nonionic–anionic surfactant (oligo(ethylene glycol) alkyl-3-sulfopropyl diether potassium salt, i.e., C m H 2 m +1 -(OC 2 H 4 ) n -C 3 H 6 –SO 3 – K + , subsequently denoted as AES) was acquired from Raschig GmbH (type F11–13) ( Figure a).

Techniques: Blocking Assay, QCM-D, Adsorption