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npas3  (Bioss)


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    Bioss npas3
    Npas3, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/npas3/product/Bioss
    Average 94 stars, based on 1 article reviews
    npas3 - by Bioz Stars, 2026-02
    94/100 stars

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    Image Search Results


    a. Antibody staining for BRN2 (Layers II/II), CTIP2 (Layers V), and FOXP2 (Layers VI) layer-specific neuronal markers in the P7 cortex from Nfia-cKO and control; quantification is derived from n = 3 pairs of animals (6 images, GLME model). b. CX-specific transcription factor DEGs increased between P7-P14. c. OB-specific transcription factor DEGs increased between P7-P14. d. Immunoprecipitation of LHX2 and immunoblot of LHX2 and NFIA from the P28 cortex; arrow heads label the proteins of interest. e. Immunoprecipitation of NPAS3 and immunoblot of NPAS3 and SOX9 from P28 cortex; arrow heads label the proteins of interest. f. Quantification of LHX2 expression in virally labeled astrocytes from the P28 cortex of mice where it has been knocked out using guideRNAs in the ROSA-LSL-Cas9-EGFP mouse line; quantification is derived from n = 3 pairs of animals (37, 39 cells; GLME model, ***P = 0.00099). g. Imaging of virally labeled astrocytes from the P28 cortex of Lhx2-cKO mice where Lhx2 has been knocked out using guideRNAs in the ROSA-LSL-Cas9-EGFP mouse line; quantification of morphological complexity via Scholl analysis was derived from n = 3 animals (Lhx2-cKO: 13 mcherry–Cas9-EGFP+ cells, 40 mcherry+Cas9-EGFP+ cells; GLME model with Sidak’s multiple comparisons test, **P = 0.0037; GLME model, **P = 0.006, 0.003). h. RNAscope for Gabbr1 expression in Cas9-EGFP cortical astrocytes lacking Lhx2 and controls at P28; quantitative analysis demonstrating reduction of Gabbr1 expression is derived from n = 3 pairs of animals (51,55 cells; GLME model, *P = 0.015; LME model, **P = 0.0003). Scale bars, 100 μm (a), 20 μm (m). Data represent mean ± s.d. (a, g right), median, minimum value, maximum value and IQR (f, g left, h).

    Journal: Nature

    Article Title: Inhibitory input directs astrocyte morphogenesis through glial GABA B R

    doi: 10.1038/s41586-023-06010-x

    Figure Lengend Snippet: a. Antibody staining for BRN2 (Layers II/II), CTIP2 (Layers V), and FOXP2 (Layers VI) layer-specific neuronal markers in the P7 cortex from Nfia-cKO and control; quantification is derived from n = 3 pairs of animals (6 images, GLME model). b. CX-specific transcription factor DEGs increased between P7-P14. c. OB-specific transcription factor DEGs increased between P7-P14. d. Immunoprecipitation of LHX2 and immunoblot of LHX2 and NFIA from the P28 cortex; arrow heads label the proteins of interest. e. Immunoprecipitation of NPAS3 and immunoblot of NPAS3 and SOX9 from P28 cortex; arrow heads label the proteins of interest. f. Quantification of LHX2 expression in virally labeled astrocytes from the P28 cortex of mice where it has been knocked out using guideRNAs in the ROSA-LSL-Cas9-EGFP mouse line; quantification is derived from n = 3 pairs of animals (37, 39 cells; GLME model, ***P = 0.00099). g. Imaging of virally labeled astrocytes from the P28 cortex of Lhx2-cKO mice where Lhx2 has been knocked out using guideRNAs in the ROSA-LSL-Cas9-EGFP mouse line; quantification of morphological complexity via Scholl analysis was derived from n = 3 animals (Lhx2-cKO: 13 mcherry–Cas9-EGFP+ cells, 40 mcherry+Cas9-EGFP+ cells; GLME model with Sidak’s multiple comparisons test, **P = 0.0037; GLME model, **P = 0.006, 0.003). h. RNAscope for Gabbr1 expression in Cas9-EGFP cortical astrocytes lacking Lhx2 and controls at P28; quantitative analysis demonstrating reduction of Gabbr1 expression is derived from n = 3 pairs of animals (51,55 cells; GLME model, *P = 0.015; LME model, **P = 0.0003). Scale bars, 100 μm (a), 20 μm (m). Data represent mean ± s.d. (a, g right), median, minimum value, maximum value and IQR (f, g left, h).

    Article Snippet: The following primary antibodies were used: Chicken anti-GFP (1:1000; Abcam, ab13970), rabbit anti-NFIA (1:500; Sigma, HPA006111), chicken anti-GFAP (1:1000; Abcam, ab4674), mouse anti-GFAP (1:1000; EMD Millipore, MAB360), goat anti-SOX9 (1:750; RD system, AF3075), rabbit anti-SOX9 (1:650; EMD Millipore, AB5535), rabbit anti-BRN2 (1:1000; Cell Signaling Technology, 12137S), rat anti-CTIP2 (1:500; Abcam, ab18465), rabbit anti-FOXP2 (1:500; Abcam, ab16046), mouse anti-GAD67 (1:200; EMD Millipore, MAB5406), mouse anti-Gephyrin (1:600; Synaptic Systems, 147011), rat anti-HA (1:100; Sigma, 11867423001), rabbit anti-PSD95 (1:200; Thermo Fisher Scientific, 51–6900), guinea pig anti-VGAT (1:350; Synaptic Systems, 131004), guinea pig anti-VGlut1 (1:2000; EMD Millipore, AB5905), guinea pig anti-VGlut2 (1:5000; EMD Millipore, AB2251), rabbit anti-EDNRB (1:250, Abcam, ab117529), rabbit anti-LHX2 (1:250, Abcam, ab184337), rabbit anti-NPAS3 (1:250; Thermo Fisher, PA5–20365).

    Techniques: Immunoprecipitation, Staining, Derivative Assay, Western Blot, Expressing, Labeling, Imaging

    a. Imaging of Aldh1l1-GFP astrocytes at P28 from the Sox9-cKO and Nfia-cKO; quantification via Scholl analysis was derived from n = 3 pairs of animals (Nfia control: OB 56, CX 52, Nfia cKO: OB 60, CX 48, Sox9 control: OB 29, CX 35, Sox9 cKO: OB 39, CX 33 cells; GLME model with Sidak’s multiple comparisons test, *P = 0.015, P = 0.41, 0.60, **P = 0.0097). b. Immunostaining for LHX2 in P28 astrocytes quantified from n = 3 pairs of animals (LME model, ****P = 1.31e-12). c. Immunostaining for NPAS3 in P28 astrocytes quantified from n = 3 pairs of animals (GLME model, **P = 0.0013). d-g. Imaging of virally labeled astrocytes lacking Lhx2 from P28 cortex; quantification via Scholl analysis was derived from n = 3 pairs of animals (41,41 cells; f, GLME model with Sidak’s multiple comparisons test, ****P = 1.29e-24; g, GLME model, ***P = 0.00099, ****P = 3.98e-05). Scale bars, 30 μm (a, e), 20 μm (b, c). Data represent mean ± s.d. (a, f), median, minimum value, maximum value and IQR (b, c, g,).

    Journal: Nature

    Article Title: Inhibitory input directs astrocyte morphogenesis through glial GABA B R

    doi: 10.1038/s41586-023-06010-x

    Figure Lengend Snippet: a. Imaging of Aldh1l1-GFP astrocytes at P28 from the Sox9-cKO and Nfia-cKO; quantification via Scholl analysis was derived from n = 3 pairs of animals (Nfia control: OB 56, CX 52, Nfia cKO: OB 60, CX 48, Sox9 control: OB 29, CX 35, Sox9 cKO: OB 39, CX 33 cells; GLME model with Sidak’s multiple comparisons test, *P = 0.015, P = 0.41, 0.60, **P = 0.0097). b. Immunostaining for LHX2 in P28 astrocytes quantified from n = 3 pairs of animals (LME model, ****P = 1.31e-12). c. Immunostaining for NPAS3 in P28 astrocytes quantified from n = 3 pairs of animals (GLME model, **P = 0.0013). d-g. Imaging of virally labeled astrocytes lacking Lhx2 from P28 cortex; quantification via Scholl analysis was derived from n = 3 pairs of animals (41,41 cells; f, GLME model with Sidak’s multiple comparisons test, ****P = 1.29e-24; g, GLME model, ***P = 0.00099, ****P = 3.98e-05). Scale bars, 30 μm (a, e), 20 μm (b, c). Data represent mean ± s.d. (a, f), median, minimum value, maximum value and IQR (b, c, g,).

    Article Snippet: The following primary antibodies were used: Chicken anti-GFP (1:1000; Abcam, ab13970), rabbit anti-NFIA (1:500; Sigma, HPA006111), chicken anti-GFAP (1:1000; Abcam, ab4674), mouse anti-GFAP (1:1000; EMD Millipore, MAB360), goat anti-SOX9 (1:750; RD system, AF3075), rabbit anti-SOX9 (1:650; EMD Millipore, AB5535), rabbit anti-BRN2 (1:1000; Cell Signaling Technology, 12137S), rat anti-CTIP2 (1:500; Abcam, ab18465), rabbit anti-FOXP2 (1:500; Abcam, ab16046), mouse anti-GAD67 (1:200; EMD Millipore, MAB5406), mouse anti-Gephyrin (1:600; Synaptic Systems, 147011), rat anti-HA (1:100; Sigma, 11867423001), rabbit anti-PSD95 (1:200; Thermo Fisher Scientific, 51–6900), guinea pig anti-VGAT (1:350; Synaptic Systems, 131004), guinea pig anti-VGlut1 (1:2000; EMD Millipore, AB5905), guinea pig anti-VGlut2 (1:5000; EMD Millipore, AB2251), rabbit anti-EDNRB (1:250, Abcam, ab117529), rabbit anti-LHX2 (1:250, Abcam, ab184337), rabbit anti-NPAS3 (1:250; Thermo Fisher, PA5–20365).

    Techniques: Imaging, Derivative Assay, Immunostaining, Labeling