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human normal lung fibroblast cell line mrc 5  (ATCC)


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    ATCC human normal lung fibroblast cell line mrc 5
    Human Normal Lung Fibroblast Cell Line Mrc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5468 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human normal lung fibroblast cell line mrc 5/product/ATCC
    Average 99 stars, based on 5468 article reviews
    human normal lung fibroblast cell line mrc 5 - by Bioz Stars, 2025-12
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    (A) Antibody loading capacity and PLL content per mg of polymersomes. (B–C) Cellular uptake in primary human lung fibroblasts assessed by (B) flow cytometry and (C) immunohistochemistry. (D) Cytotoxicity analysis by MTT assay. Data represented as Mean ± SEM.

    Journal: bioRxiv

    Article Title: Intracellular Delivery of Full-Length Antibodies via Poly-L-lysine-Coated PEG–PLGA Polymersomes Enables Non-invasive Pulmonary Immunotherapy

    doi: 10.1101/2025.09.16.676558

    Figure Lengend Snippet: (A) Antibody loading capacity and PLL content per mg of polymersomes. (B–C) Cellular uptake in primary human lung fibroblasts assessed by (B) flow cytometry and (C) immunohistochemistry. (D) Cytotoxicity analysis by MTT assay. Data represented as Mean ± SEM.

    Article Snippet: PLL 30KDa (Sigma-Aldrich, P2636); Anti-human IgG AF488 (SoutherenBiotech; 2030-40); Primary human Lung Fibroblasts (ATCC, PCS-201-013); Fibroblast Basal Media (ATCC, PCS-201-030); Fibroblast growth Kit (ATCC, PCS-201-041), NLRP3 Ab (Novus Biologicals; NBP2-12446); Anti mouse Caspase 3 antibody, Anti-Rabbit IgG Cy5 conjugated, MTT assay kit (Invitrogen, V13154), IL-1β Elisa Kit (Invitrogen, DY201-05), DAPI (Invitrogen, D 3571), BCA assay Kit (Thermo Fischer, 23227), Uranyl Acetate, TEM Grid, 300 kDa Float-A-Lyzer (Biotech CE Trial kit dialysis membrane).

    Techniques: Flow Cytometry, Immunohistochemistry, MTT Assay

    (A) Schematic of experimental design using primary human lung fibroblasts. (B) Quantification of IL-1β levels in culture supernatants by ELISA, normalized to total protein. (C) Representative flow cytometry plots from Live/Dead assay following NLRP3 induction and treatment with NLRP3 Ab–loaded PLL-PS, blank PLL-PS, or untreated healthy cells. (D) Quantification of live cells across treatment groups. Data shown as Mean ± SEM.

    Journal: bioRxiv

    Article Title: Intracellular Delivery of Full-Length Antibodies via Poly-L-lysine-Coated PEG–PLGA Polymersomes Enables Non-invasive Pulmonary Immunotherapy

    doi: 10.1101/2025.09.16.676558

    Figure Lengend Snippet: (A) Schematic of experimental design using primary human lung fibroblasts. (B) Quantification of IL-1β levels in culture supernatants by ELISA, normalized to total protein. (C) Representative flow cytometry plots from Live/Dead assay following NLRP3 induction and treatment with NLRP3 Ab–loaded PLL-PS, blank PLL-PS, or untreated healthy cells. (D) Quantification of live cells across treatment groups. Data shown as Mean ± SEM.

    Article Snippet: PLL 30KDa (Sigma-Aldrich, P2636); Anti-human IgG AF488 (SoutherenBiotech; 2030-40); Primary human Lung Fibroblasts (ATCC, PCS-201-013); Fibroblast Basal Media (ATCC, PCS-201-030); Fibroblast growth Kit (ATCC, PCS-201-041), NLRP3 Ab (Novus Biologicals; NBP2-12446); Anti mouse Caspase 3 antibody, Anti-Rabbit IgG Cy5 conjugated, MTT assay kit (Invitrogen, V13154), IL-1β Elisa Kit (Invitrogen, DY201-05), DAPI (Invitrogen, D 3571), BCA assay Kit (Thermo Fischer, 23227), Uranyl Acetate, TEM Grid, 300 kDa Float-A-Lyzer (Biotech CE Trial kit dialysis membrane).

    Techniques: Enzyme-linked Immunosorbent Assay, Flow Cytometry, Live Dead Assay