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nbqx  (Tocris)


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    Structured Review

    Tocris nbqx
    (A) Example trace of sIPSC recordings performed with caesium chloride (CsCl) and in the presence of the AMPA and NMDA blockers <t>NBQX</t> <t>and</t> <t>DL-AP5,</t> respectively. (B) Boxplots of sIPSC frequency, amplitude, rise time, and decay time (frequency: 4.73 ± 2.09 Hz; amplitude:-62.5 ± 25.6 pA; rise time; 2.00 ± 0.31 ms; decay time: 5.27 ± 1.34 ms; n = 15 neurons from 5 mice). (C) Example traces of a recorded MDJ-IO neuron before (t=0), 10 minutes after (t=10), and 20 minutes (t=20) after the addition of the GABA receptor blocker picrotoxin (PTX). (D) Time-course of the frequency minutes (F(1,6.0687) = 156.47, p = 1.466e-5, linear mixed model), amplitude (F(1, 2.702) = 58.028, p = 0.0067, linear mixed model), rise time (F(1, 4.9424) = 11.812, p = 0.0188, linear mixed model) and decay time (F(1,4.9115) = 0.98111, p = 0.3682, linear mixed model) of sIPSCs after the addition of PTX (red dotted line). (E-F) Confocal images of the MDJ labelled with fluorescent markers for VGAT (left) and Gephyrin (middle), and overlayed together (right). The image in (e) is the same animal as in (f), but (f) is at a higher magnification and resolution. Data are represented as mean ± SD.
    Nbqx, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1990 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nbqx/NBQX/bio_rxiv__64898__2026__04__10__717667-71-39-40
    Average 96 stars, based on 1990 article reviews
    nbqx - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Electrophysiological properties of mesodiencephalic junction neurons projecting to the inferior olive"

    Article Title: Electrophysiological properties of mesodiencephalic junction neurons projecting to the inferior olive

    Journal: bioRxiv

    doi: 10.64898/2026.04.10.717667

    (A) Example trace of sIPSC recordings performed with caesium chloride (CsCl) and in the presence of the AMPA and NMDA blockers NBQX and DL-AP5, respectively. (B) Boxplots of sIPSC frequency, amplitude, rise time, and decay time (frequency: 4.73 ± 2.09 Hz; amplitude:-62.5 ± 25.6 pA; rise time; 2.00 ± 0.31 ms; decay time: 5.27 ± 1.34 ms; n = 15 neurons from 5 mice). (C) Example traces of a recorded MDJ-IO neuron before (t=0), 10 minutes after (t=10), and 20 minutes (t=20) after the addition of the GABA receptor blocker picrotoxin (PTX). (D) Time-course of the frequency minutes (F(1,6.0687) = 156.47, p = 1.466e-5, linear mixed model), amplitude (F(1, 2.702) = 58.028, p = 0.0067, linear mixed model), rise time (F(1, 4.9424) = 11.812, p = 0.0188, linear mixed model) and decay time (F(1,4.9115) = 0.98111, p = 0.3682, linear mixed model) of sIPSCs after the addition of PTX (red dotted line). (E-F) Confocal images of the MDJ labelled with fluorescent markers for VGAT (left) and Gephyrin (middle), and overlayed together (right). The image in (e) is the same animal as in (f), but (f) is at a higher magnification and resolution. Data are represented as mean ± SD.
    Figure Legend Snippet: (A) Example trace of sIPSC recordings performed with caesium chloride (CsCl) and in the presence of the AMPA and NMDA blockers NBQX and DL-AP5, respectively. (B) Boxplots of sIPSC frequency, amplitude, rise time, and decay time (frequency: 4.73 ± 2.09 Hz; amplitude:-62.5 ± 25.6 pA; rise time; 2.00 ± 0.31 ms; decay time: 5.27 ± 1.34 ms; n = 15 neurons from 5 mice). (C) Example traces of a recorded MDJ-IO neuron before (t=0), 10 minutes after (t=10), and 20 minutes (t=20) after the addition of the GABA receptor blocker picrotoxin (PTX). (D) Time-course of the frequency minutes (F(1,6.0687) = 156.47, p = 1.466e-5, linear mixed model), amplitude (F(1, 2.702) = 58.028, p = 0.0067, linear mixed model), rise time (F(1, 4.9424) = 11.812, p = 0.0188, linear mixed model) and decay time (F(1,4.9115) = 0.98111, p = 0.3682, linear mixed model) of sIPSCs after the addition of PTX (red dotted line). (E-F) Confocal images of the MDJ labelled with fluorescent markers for VGAT (left) and Gephyrin (middle), and overlayed together (right). The image in (e) is the same animal as in (f), but (f) is at a higher magnification and resolution. Data are represented as mean ± SD.

    Techniques Used:

    Related Articles

    other:

    Article Title: Enrichment experience improves hippocampal sparse coding via inhibitory circuit plasticity
    Article Snippet: Stock solutions of NBQX (Tocris Bioscience, 10 mM), D-AP5 (Tocris Bioscience, 25 mM), Gabazine (SR 95531 hydrobromide, Sigma-Aldrich, 10 mM) and Tetrodotoxin (TTX, Alomone Labs, 1 mM) were dissolved in water.

    Transmission Assay:

    Article Title: Vagal sensory neurones directly synapse onto parasympathetic motor (DMV) neurones
    Article Snippet: The laser was calibrated using a silicon photodiode (S120C; Thorlabs, Newton, NJ, USA) and power sensor (PM100D; Thorlabs) to emit light at a power of 15 mW. .. TTX (3 μm; catalog. no. 1078; Tocris, Bristiol, UK), 4-aminopyridine (4-AP, 25 μm; catalog. no. A78403; Merck Sigma-Aldrich, Burlington, MA, USA), NBQX (20 μM; catalog. no. 1044; Tocris) and gabazine (3 μm, SR-95531; catalog. no. 1262; Tocris) were used to alter synaptic transmission or transmitter function in the assay. ..

    Blocking Assay:

    Article Title: Compound- and sex-specific medial prefrontal cortex rewiring after prenatal THC and CBD exposure
    Article Snippet: GABA-sIPSCs were recorded at − 70 mV in presence of 20 μM CNQX (6-Cyano-7-nitroquinoxaline-2,3-dione disodium, an AMPA receptor antagonist, Tocris) and L-APV 50 μM (DL-2-Amino-5-phosphonopentanoic acid, a selective NMDA receptor antagonist, Tocris). .. NMDA-sEPSCs: Recorded at +30 mV in the presence of 20 μM NBQX (2,3-dioxo-6-nitro-7-sulfamoyl-benzo[f]quinoxaline, Tocris) to block AMPA and kainite mediated currents and using a cesium-methanesulfonate internal solution (in mM: cesium-methanesulfonate (143), NaCl (5), MgCl2(1), EGTA (1), CaCl2 (0.3), Hepes (10), Na2ATP (2), NaGTP (0.3) and cAMP (0.2) (pH 7.3 and 290 mOsm). ..

    Article Title: Cerebellar microglia-derived IL-17A mitigates autism-related behavioral and synaptic deficits.
    Article Snippet: .. For sIPSCs recordings, glass pipettes were loaded with high Cl− internal solution containing 135mM CsCl, 2 mM NaCl, 1 mM MgSO4, 0.2 mM CaCl2, 0.2 mM EGTA, 5 mM QX314, 4 mM Na2ATP, 0.4 mM Na-GTP, with the pH adjusted to 7.2 using CsOH; D-APV (50 μM; Tocris Bioscience, 0106) and NBQX (20 μM; Tocris Bioscience, 0373) were included to block NMDA and AMPA receptors, respectively. ..



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    (A) Example trace of sIPSC recordings performed with caesium chloride (CsCl) and in the presence of the AMPA and NMDA blockers <t>NBQX</t> <t>and</t> <t>DL-AP5,</t> respectively. (B) Boxplots of sIPSC frequency, amplitude, rise time, and decay time (frequency: 4.73 ± 2.09 Hz; amplitude:-62.5 ± 25.6 pA; rise time; 2.00 ± 0.31 ms; decay time: 5.27 ± 1.34 ms; n = 15 neurons from 5 mice). (C) Example traces of a recorded MDJ-IO neuron before (t=0), 10 minutes after (t=10), and 20 minutes (t=20) after the addition of the GABA receptor blocker picrotoxin (PTX). (D) Time-course of the frequency minutes (F(1,6.0687) = 156.47, p = 1.466e-5, linear mixed model), amplitude (F(1, 2.702) = 58.028, p = 0.0067, linear mixed model), rise time (F(1, 4.9424) = 11.812, p = 0.0188, linear mixed model) and decay time (F(1,4.9115) = 0.98111, p = 0.3682, linear mixed model) of sIPSCs after the addition of PTX (red dotted line). (E-F) Confocal images of the MDJ labelled with fluorescent markers for VGAT (left) and Gephyrin (middle), and overlayed together (right). The image in (e) is the same animal as in (f), but (f) is at a higher magnification and resolution. Data are represented as mean ± SD.
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    (A) Example trace of sIPSC recordings performed with caesium chloride (CsCl) and in the presence of the AMPA and NMDA blockers <t>NBQX</t> <t>and</t> <t>DL-AP5,</t> respectively. (B) Boxplots of sIPSC frequency, amplitude, rise time, and decay time (frequency: 4.73 ± 2.09 Hz; amplitude:-62.5 ± 25.6 pA; rise time; 2.00 ± 0.31 ms; decay time: 5.27 ± 1.34 ms; n = 15 neurons from 5 mice). (C) Example traces of a recorded MDJ-IO neuron before (t=0), 10 minutes after (t=10), and 20 minutes (t=20) after the addition of the GABA receptor blocker picrotoxin (PTX). (D) Time-course of the frequency minutes (F(1,6.0687) = 156.47, p = 1.466e-5, linear mixed model), amplitude (F(1, 2.702) = 58.028, p = 0.0067, linear mixed model), rise time (F(1, 4.9424) = 11.812, p = 0.0188, linear mixed model) and decay time (F(1,4.9115) = 0.98111, p = 0.3682, linear mixed model) of sIPSCs after the addition of PTX (red dotted line). (E-F) Confocal images of the MDJ labelled with fluorescent markers for VGAT (left) and Gephyrin (middle), and overlayed together (right). The image in (e) is the same animal as in (f), but (f) is at a higher magnification and resolution. Data are represented as mean ± SD.
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    (A) Example trace of sIPSC recordings performed with caesium chloride (CsCl) and in the presence of the AMPA and NMDA blockers <t>NBQX</t> <t>and</t> <t>DL-AP5,</t> respectively. (B) Boxplots of sIPSC frequency, amplitude, rise time, and decay time (frequency: 4.73 ± 2.09 Hz; amplitude:-62.5 ± 25.6 pA; rise time; 2.00 ± 0.31 ms; decay time: 5.27 ± 1.34 ms; n = 15 neurons from 5 mice). (C) Example traces of a recorded MDJ-IO neuron before (t=0), 10 minutes after (t=10), and 20 minutes (t=20) after the addition of the GABA receptor blocker picrotoxin (PTX). (D) Time-course of the frequency minutes (F(1,6.0687) = 156.47, p = 1.466e-5, linear mixed model), amplitude (F(1, 2.702) = 58.028, p = 0.0067, linear mixed model), rise time (F(1, 4.9424) = 11.812, p = 0.0188, linear mixed model) and decay time (F(1,4.9115) = 0.98111, p = 0.3682, linear mixed model) of sIPSCs after the addition of PTX (red dotted line). (E-F) Confocal images of the MDJ labelled with fluorescent markers for VGAT (left) and Gephyrin (middle), and overlayed together (right). The image in (e) is the same animal as in (f), but (f) is at a higher magnification and resolution. Data are represented as mean ± SD.
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    (A) Example trace of sIPSC recordings performed with caesium chloride (CsCl) and in the presence of the AMPA and NMDA blockers <t>NBQX</t> <t>and</t> <t>DL-AP5,</t> respectively. (B) Boxplots of sIPSC frequency, amplitude, rise time, and decay time (frequency: 4.73 ± 2.09 Hz; amplitude:-62.5 ± 25.6 pA; rise time; 2.00 ± 0.31 ms; decay time: 5.27 ± 1.34 ms; n = 15 neurons from 5 mice). (C) Example traces of a recorded MDJ-IO neuron before (t=0), 10 minutes after (t=10), and 20 minutes (t=20) after the addition of the GABA receptor blocker picrotoxin (PTX). (D) Time-course of the frequency minutes (F(1,6.0687) = 156.47, p = 1.466e-5, linear mixed model), amplitude (F(1, 2.702) = 58.028, p = 0.0067, linear mixed model), rise time (F(1, 4.9424) = 11.812, p = 0.0188, linear mixed model) and decay time (F(1,4.9115) = 0.98111, p = 0.3682, linear mixed model) of sIPSCs after the addition of PTX (red dotted line). (E-F) Confocal images of the MDJ labelled with fluorescent markers for VGAT (left) and Gephyrin (middle), and overlayed together (right). The image in (e) is the same animal as in (f), but (f) is at a higher magnification and resolution. Data are represented as mean ± SD.
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    (A) Example trace of sIPSC recordings performed with caesium chloride (CsCl) and in the presence of the AMPA and NMDA blockers <t>NBQX</t> <t>and</t> <t>DL-AP5,</t> respectively. (B) Boxplots of sIPSC frequency, amplitude, rise time, and decay time (frequency: 4.73 ± 2.09 Hz; amplitude:-62.5 ± 25.6 pA; rise time; 2.00 ± 0.31 ms; decay time: 5.27 ± 1.34 ms; n = 15 neurons from 5 mice). (C) Example traces of a recorded MDJ-IO neuron before (t=0), 10 minutes after (t=10), and 20 minutes (t=20) after the addition of the GABA receptor blocker picrotoxin (PTX). (D) Time-course of the frequency minutes (F(1,6.0687) = 156.47, p = 1.466e-5, linear mixed model), amplitude (F(1, 2.702) = 58.028, p = 0.0067, linear mixed model), rise time (F(1, 4.9424) = 11.812, p = 0.0188, linear mixed model) and decay time (F(1,4.9115) = 0.98111, p = 0.3682, linear mixed model) of sIPSCs after the addition of PTX (red dotted line). (E-F) Confocal images of the MDJ labelled with fluorescent markers for VGAT (left) and Gephyrin (middle), and overlayed together (right). The image in (e) is the same animal as in (f), but (f) is at a higher magnification and resolution. Data are represented as mean ± SD.
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    Image Search Results


    (A) Example trace of sIPSC recordings performed with caesium chloride (CsCl) and in the presence of the AMPA and NMDA blockers NBQX and DL-AP5, respectively. (B) Boxplots of sIPSC frequency, amplitude, rise time, and decay time (frequency: 4.73 ± 2.09 Hz; amplitude:-62.5 ± 25.6 pA; rise time; 2.00 ± 0.31 ms; decay time: 5.27 ± 1.34 ms; n = 15 neurons from 5 mice). (C) Example traces of a recorded MDJ-IO neuron before (t=0), 10 minutes after (t=10), and 20 minutes (t=20) after the addition of the GABA receptor blocker picrotoxin (PTX). (D) Time-course of the frequency minutes (F(1,6.0687) = 156.47, p = 1.466e-5, linear mixed model), amplitude (F(1, 2.702) = 58.028, p = 0.0067, linear mixed model), rise time (F(1, 4.9424) = 11.812, p = 0.0188, linear mixed model) and decay time (F(1,4.9115) = 0.98111, p = 0.3682, linear mixed model) of sIPSCs after the addition of PTX (red dotted line). (E-F) Confocal images of the MDJ labelled with fluorescent markers for VGAT (left) and Gephyrin (middle), and overlayed together (right). The image in (e) is the same animal as in (f), but (f) is at a higher magnification and resolution. Data are represented as mean ± SD.

    Journal: bioRxiv

    Article Title: Electrophysiological properties of mesodiencephalic junction neurons projecting to the inferior olive

    doi: 10.64898/2026.04.10.717667

    Figure Lengend Snippet: (A) Example trace of sIPSC recordings performed with caesium chloride (CsCl) and in the presence of the AMPA and NMDA blockers NBQX and DL-AP5, respectively. (B) Boxplots of sIPSC frequency, amplitude, rise time, and decay time (frequency: 4.73 ± 2.09 Hz; amplitude:-62.5 ± 25.6 pA; rise time; 2.00 ± 0.31 ms; decay time: 5.27 ± 1.34 ms; n = 15 neurons from 5 mice). (C) Example traces of a recorded MDJ-IO neuron before (t=0), 10 minutes after (t=10), and 20 minutes (t=20) after the addition of the GABA receptor blocker picrotoxin (PTX). (D) Time-course of the frequency minutes (F(1,6.0687) = 156.47, p = 1.466e-5, linear mixed model), amplitude (F(1, 2.702) = 58.028, p = 0.0067, linear mixed model), rise time (F(1, 4.9424) = 11.812, p = 0.0188, linear mixed model) and decay time (F(1,4.9115) = 0.98111, p = 0.3682, linear mixed model) of sIPSCs after the addition of PTX (red dotted line). (E-F) Confocal images of the MDJ labelled with fluorescent markers for VGAT (left) and Gephyrin (middle), and overlayed together (right). The image in (e) is the same animal as in (f), but (f) is at a higher magnification and resolution. Data are represented as mean ± SD.

    Article Snippet: Recordings of sIPSCs were done using a caesium-chloride internal containing in mM: 150 CsCl, 1.5 MgCl2, 0.5 EGTA, 10 HEPES, 4 Na2ATP, 0.3 Na3GTP, 5 Qx-314. sIPSCs were recorded with 1 μM TTX (HelloBio, Bristol, United Kingdom), 20 μM NBQX (Tocris Bioscience, Bristol, United Kingdom), and 50 μM D-AP5 or DL-AP5 (Tocris Bioscience).

    Techniques: