Journal: PLoS ONE

Article Title: Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis

doi: 10.1371/journal.pone.0059237

Figure Lengend Snippet: A majority consensus mid-point rooted maximum parsimony (MP) tree was constructed based on the concatenated sequence of seven MLST gene loci. The MP tree is displayed without logarithmic scaling so that genetic distance and geographic region of isolation could be viewed. Values along branches represent maximum parsimony bootstrap values (MPB) and Bayesian posterior probability (BPP) values respectively. Values for branches partitioning less than three isolates or those with MPB ≤70 and BPP≤0.95 are not shown. The tree is displayed with pattern coding for geographic regions as described in . The MP tree displays a partition in the current species B. dermatitidis into two phylogenetic species: B. dermatitidis (PS1, clade 1) and B. gilchristii (PS2, clade 2).

Article Snippet: Differences in growth on various media have also been documented for select B. dermatitidis (PS1) (ATCC MYA-2586 (ER-3)) and B. gilchristii (PS2) (ATCC MYA- 2585 (ERC-2)) isolates at 20°C and 37°C .

Techniques: Construct, Sequencing, Isolation

Journal: PLoS ONE

Article Title: Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis

doi: 10.1371/journal.pone.0059237

Figure Lengend Snippet: Linkage disequilibrium measurements for all isolates and unique genotypes in the entire population, the entire population partitioned into two phylogenetic species or six geographic regions, and the population within each phylogenetic species ( B. dermatitidis (PS1) and B. gilchristii (PS2)).

Article Snippet: Differences in growth on various media have also been documented for select B. dermatitidis (PS1) (ATCC MYA-2586 (ER-3)) and B. gilchristii (PS2) (ATCC MYA- 2585 (ERC-2)) isolates at 20°C and 37°C .

Techniques:

Journal: PLoS ONE

Article Title: Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis

doi: 10.1371/journal.pone.0059237

Figure Lengend Snippet: of the B. dermatitidis. Split decomposition analysis was performed using the pairwise genetic distances of the concatenated chs2-drk1-fads-pyrF-tub1-arf6-its2 sequences. Isolated split networks within B. gilchristii (PS2) suggests recombination within the B. gilchristii (PS2) but not between both populations. Bootstrapping values less than ≤0.75 are not shown.

Article Snippet: Differences in growth on various media have also been documented for select B. dermatitidis (PS1) (ATCC MYA-2586 (ER-3)) and B. gilchristii (PS2) (ATCC MYA- 2585 (ERC-2)) isolates at 20°C and 37°C .

Techniques: Isolation

Journal: PLoS ONE

Article Title: Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis

doi: 10.1371/journal.pone.0059237

Figure Lengend Snippet: Intra- and interlocus simple four-gametes tests of each locus for B. dermatitidis (PS1) and B. gilchristii (PS2).

Article Snippet: Differences in growth on various media have also been documented for select B. dermatitidis (PS1) (ATCC MYA-2586 (ER-3)) and B. gilchristii (PS2) (ATCC MYA- 2585 (ERC-2)) isolates at 20°C and 37°C .

Techniques:

Journal: PLoS ONE

Article Title: Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis

doi: 10.1371/journal.pone.0059237

Figure Lengend Snippet: Phylogenetic species are represented by circles with light grey ( B. dermatitidis , PS1) and solid ( B. gilchristii , PS2) fill corresponding to each phylogenetic species. Circle size is proportional to the number of isolates from each geographic site.

Article Snippet: Differences in growth on various media have also been documented for select B. dermatitidis (PS1) (ATCC MYA-2586 (ER-3)) and B. gilchristii (PS2) (ATCC MYA- 2585 (ERC-2)) isolates at 20°C and 37°C .

Techniques: