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mitosox red mitochondrial superoxide indicator  (MedChemExpress)


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    MedChemExpress mitosox red mitochondrial superoxide indicator
    Mitosox Red Mitochondrial Superoxide Indicator, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mitochondri/MitoSOX+Red/pm42570014-45-33-38
    Average 99 stars, based on 450 article reviews
    mitosox red mitochondrial superoxide indicator - by Bioz Stars, 2026-09
    99/100 stars

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    other:

    Article Title: Mitochondrial Haplogroups Influence Mitochondrial Structure and Function, Oxidative Stress, Autophagy, and Lipid Metabolism of Chicken Hepatocytes in Response to Energy Stimulation.
    Article Snippet: Primary antibodies used in this study include Mitofusin 2 (MFN2) (sc-100560, Santa Cruz Biotechnology, Dallas, TX, USA), Beta-Actin (β-actin) (AC026, ABclonal, Wuhan, China), Superoxide Dismutase 2 (SOD2) (24127-1-AP, Proteintech, Wuhan, China), Microtubule-Associated Protein 1A/1B-Light Chain 3 Beta (LC3B) (3868T, Cell Signaling Technology, Danvers, MA, USA), Mitochondri- https://doi.org/10.3390/ani16050766 ally Encoded NADH Dehydrogenase 1 (MT-ND1) (YA2089, MedChemExpress, Monmouth Junction, NJ, USA), and Cytochrome B (CYTB) (55090-1-AP, Proteintech, Wuhan, China).



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    jc 1 assay kit  (MedChemExpress)
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    MedChemExpress jc 1 assay kit
    MCU regulates mitophagy and mitochondrial function. A. GSEA analysis showed that MCU-associated genes were significantly enriched in the “autophagy of mitochondrion” and “positive regulation of autophagy of mitochondrion” pathways; B. Western blot analysis was performed to detect the expression of mitophagy-related proteins, including PINK1, PRKN, and LC3B, in H1975 and PC9 cells; C, D. Immunofluorescence analysis was used to examine the colocalization of mitochondria and LC3B in H1975 and PC9 cells; E, <t>F.</t> <t>JC-1</t> staining combined with flow cytometry was performed to assess changes in mitochondrial membrane potential in H1975 and PC9 cells; G, H. Flow cytometry was used to detect intracellular ROS levels in H1975 and PC9 cells. Data are presented as the mean ± SD. Statistical analysis was performed using two-sided t-tests. **P < 0.01; ***P < 0.001; ****P < 0.0001.
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    MCU regulates mitophagy and mitochondrial function. A. GSEA analysis showed that MCU-associated genes were significantly enriched in the “autophagy of mitochondrion” and “positive regulation of autophagy of mitochondrion” pathways; B. Western blot analysis was performed to detect the expression of mitophagy-related proteins, including PINK1, PRKN, and LC3B, in H1975 and PC9 cells; C, D. Immunofluorescence analysis was used to examine the colocalization of mitochondria and LC3B in H1975 and PC9 cells; E, F. JC-1 staining combined with flow cytometry was performed to assess changes in mitochondrial membrane potential in H1975 and PC9 cells; G, H. Flow cytometry was used to detect intracellular ROS levels in H1975 and PC9 cells. Data are presented as the mean ± SD. Statistical analysis was performed using two-sided t-tests. **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Journal: American Journal of Cancer Research

    Article Title: Mitochondrial calcium uniporter as a biomarker in lung adenocarcinoma: regulation of the immune microenvironment, apoptosis, cell cycle and mitophagy

    doi: 10.62347/PGQK3358

    Figure Lengend Snippet: MCU regulates mitophagy and mitochondrial function. A. GSEA analysis showed that MCU-associated genes were significantly enriched in the “autophagy of mitochondrion” and “positive regulation of autophagy of mitochondrion” pathways; B. Western blot analysis was performed to detect the expression of mitophagy-related proteins, including PINK1, PRKN, and LC3B, in H1975 and PC9 cells; C, D. Immunofluorescence analysis was used to examine the colocalization of mitochondria and LC3B in H1975 and PC9 cells; E, F. JC-1 staining combined with flow cytometry was performed to assess changes in mitochondrial membrane potential in H1975 and PC9 cells; G, H. Flow cytometry was used to detect intracellular ROS levels in H1975 and PC9 cells. Data are presented as the mean ± SD. Statistical analysis was performed using two-sided t-tests. **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Article Snippet: JC-1 assay kit (MedChemExpress, New Jersey, USA) is to be used for the mitochondrial membrane potential experiment.

    Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Flow Cytometry, Membrane