Journal: American Journal of Cancer Research

Article Title: Mitochondrial calcium uniporter as a biomarker in lung adenocarcinoma: regulation of the immune microenvironment, apoptosis, cell cycle and mitophagy

doi: 10.62347/PGQK3358

Figure Lengend Snippet: MCU regulates mitophagy and mitochondrial function. A. GSEA analysis showed that MCU-associated genes were significantly enriched in the “autophagy of mitochondrion” and “positive regulation of autophagy of mitochondrion” pathways; B. Western blot analysis was performed to detect the expression of mitophagy-related proteins, including PINK1, PRKN, and LC3B, in H1975 and PC9 cells; C, D. Immunofluorescence analysis was used to examine the colocalization of mitochondria and LC3B in H1975 and PC9 cells; E, F. JC-1 staining combined with flow cytometry was performed to assess changes in mitochondrial membrane potential in H1975 and PC9 cells; G, H. Flow cytometry was used to detect intracellular ROS levels in H1975 and PC9 cells. Data are presented as the mean ± SD. Statistical analysis was performed using two-sided t-tests. **P < 0.01; ***P < 0.001; ****P < 0.0001.

Article Snippet: JC-1 assay kit (MedChemExpress, New Jersey, USA) is to be used for the mitochondrial membrane potential experiment.

Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Flow Cytometry, Membrane