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Proteintech anti mff
Anti Mff, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mff/MFF+Antibody/pm41862479-335-31-33
Average 96 stars, based on 241 article reviews
anti mff - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Western Blot:

Article Title: Systematic evaluation of mitochondrial morphology regulators for amelioration of neuronal α-synucleinopathy.
Article Snippet: .. The following primary antibodies were used for Western blotting in the study: Mff (Proteintech, Rosemont, IL, 1:5000), Fis1 (Proteintech, 1:5000), NeuN (Sigma, 1:1000), GAPDH (Proteintech, 1:10000), beta-actin (Abbkine, Wuhan, China, 1:10000). .. The membranes were washed three times with 0.1% Tween20/TBS (TBST) and incubated with HRPconjugated secondary antibodies (Abbkine) for 1 h at room temperature, followed by three washes with TBST.

Article Title: CSRP3 promotes skeletal muscle remodeling toward aerobic metabolism and enhances exercise endurance through increasing LDHD activity.
Article Snippet: Exercise performance and skeletal muscle homeostasis are influenced by myofiber type composition.. Cysteine and glycine-rich protein 3 (CSRP3) is highly expressed in the oxidative fiber-rich mammalian soleus muscle.. However, the mechanistic basis of CSRP3's involvement in skeletal muscle development and myofiber type specification remains unclear.

Article Title: Systematic evaluation of mitochondrial morphology regulators for amelioration of neuronal α-synucleinopathy
Article Snippet: .. The following primary antibodies were used for Western blotting in the study: Mff (Proteintech, Rosemont, IL, 1:5000), Fis1 (Proteintech, 1:5000), NeuN (Sigma, 1:1000), GAPDH (Proteintech, 1:10000), beta-actin (Abbkine, Wuhan, China, 1:10000). .. The membranes were washed three times with 0.1% Tween20/TBS (TBST) and incubated with HRP-conjugated secondary antibodies (Abbkine) for 1 h at room temperature, followed by three washes with TBST.

Blocking Assay:

Article Title: SENP3/FIS1-regulated PFC neural mitochondrial fragmentation underlies the mechanism of electroacupuncture attenuating depressive behavior in CUMS mice
Article Snippet: .. After blocking with BSA/TBST at room temperature for 3 h, the membranes were incubated with primary antibodies, including DRP1 (1:1500, 12957-1-Ap, Proteintech, Chicago, USA), MFF (1:4000, 66527-1-Ig, Proteintech), FIS1 (1:1000, SAB2702049, Sigma-Aldrich), SUMO2/3 (1:1500, 11251-1-Ap, Proteintech), SENP3 (1:1000, 17659-1-Ap, Proteintech), and GAPDH (1:3000, HRP-60004, Proteintech), overnight at 4°C. .. Secondary antibodies were incubated at room temperature for 1 h (1:3000, anti-rabbit SA00001-2, Proteintech; 1:3000, anti-mouse SA00001-1, Proteintech).

Article Title: Nsun2-mediated m5C methylation of Ncor1 exacerbates sepsis-induced cardiomyopathy by promoting mitochondrial dysfunction.
Article Snippet: Sepsis-induced cardiomyopathy (SIC) is a severe complication of sepsis characterized by mitochondrial dysfunction and impaired myocardial contractility, yet its molecular pathogenesis remains incompletely understood.. In this study, we demonstrate that excessive mitochondrial fission plays a pivotal role in SIC, contributing to inflammation, oxidative stress, and cardiomyocyte apoptosis.. Pharmacological inhibition of mitochondrial fission using Mdivi-1 alleviated these pathological changes both in vivo and in vitro.

Incubation:

Article Title: SENP3/FIS1-regulated PFC neural mitochondrial fragmentation underlies the mechanism of electroacupuncture attenuating depressive behavior in CUMS mice
Article Snippet: .. After blocking with BSA/TBST at room temperature for 3 h, the membranes were incubated with primary antibodies, including DRP1 (1:1500, 12957-1-Ap, Proteintech, Chicago, USA), MFF (1:4000, 66527-1-Ig, Proteintech), FIS1 (1:1000, SAB2702049, Sigma-Aldrich), SUMO2/3 (1:1500, 11251-1-Ap, Proteintech), SENP3 (1:1000, 17659-1-Ap, Proteintech), and GAPDH (1:3000, HRP-60004, Proteintech), overnight at 4°C. .. Secondary antibodies were incubated at room temperature for 1 h (1:3000, anti-rabbit SA00001-2, Proteintech; 1:3000, anti-mouse SA00001-1, Proteintech).

Article Title: Nsun2-mediated m5C methylation of Ncor1 exacerbates sepsis-induced cardiomyopathy by promoting mitochondrial dysfunction.
Article Snippet: Sepsis-induced cardiomyopathy (SIC) is a severe complication of sepsis characterized by mitochondrial dysfunction and impaired myocardial contractility, yet its molecular pathogenesis remains incompletely understood.. In this study, we demonstrate that excessive mitochondrial fission plays a pivotal role in SIC, contributing to inflammation, oxidative stress, and cardiomyocyte apoptosis.. Pharmacological inhibition of mitochondrial fission using Mdivi-1 alleviated these pathological changes both in vivo and in vitro.

Article Title: CSRP3 promotes skeletal muscle remodeling toward aerobic metabolism and enhances exercise endurance through increasing LDHD activity.
Article Snippet: Exercise performance and skeletal muscle homeostasis are influenced by myofiber type composition.. Cysteine and glycine-rich protein 3 (CSRP3) is highly expressed in the oxidative fiber-rich mammalian soleus muscle.. However, the mechanistic basis of CSRP3's involvement in skeletal muscle development and myofiber type specification remains unclear.

Membrane:

Article Title: CSRP3 promotes skeletal muscle remodeling toward aerobic metabolism and enhances exercise endurance through increasing LDHD activity.
Article Snippet: Exercise performance and skeletal muscle homeostasis are influenced by myofiber type composition.. Cysteine and glycine-rich protein 3 (CSRP3) is highly expressed in the oxidative fiber-rich mammalian soleus muscle.. However, the mechanistic basis of CSRP3's involvement in skeletal muscle development and myofiber type specification remains unclear.



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Proteintech mff antibody
<t>TRIM21</t> was an E3 ubiquitin ligase of <t>MFF</t> and promoted its ubiquitination degradation. (A and B) The levels of the remained MFF protein in 40 μg/mL CIP-exposed HTR-8/SVneo cells and with 10 μM CHX treatment within 10 h and its relative quantification (n = 3 independent experiments, Student's t-test). (C) The protein levels of MFF in 40 μg/mL CIP-exposed HTR-8/SVneo cells treated with 10 μM MG132 or 50 μM CQ and co-treated with 10 μM CHX and its relative quantification (n = 3 independent experiments, one-way ANOVA). (D and E) IP assay analysis of the protein levels of MFF-Ub pulled down by MFF in 40 μg/mL CIP-exposed HTR-8/SVneo cells and its relative quantification (n = 3 independent experiments, Student's t-test). (F) MFF IP-MS assay analysis of the proteins that were pulled down by MFF in HTR-8/SVneo cells. (G–K) IP assay analysis of the protein levels of TRIM21 and MFF-Ub pulled down by MFF in HTR-8/SVneo cells with overexpression (Student's t-test) or knockdown (one-way ANOVA) of TRIM21, with MFF and TRIM21 protein levels in cell lysates and their relative quantification (n = 3 independent experiments).
Mff Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mff/MFF+Antibody/pmc12906125-270-5-8
Average 96 stars, based on 1 article reviews
mff antibody - by Bioz Stars, 2026-09
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TRIM21 was an E3 ubiquitin ligase of MFF and promoted its ubiquitination degradation. (A and B) The levels of the remained MFF protein in 40 μg/mL CIP-exposed HTR-8/SVneo cells and with 10 μM CHX treatment within 10 h and its relative quantification (n = 3 independent experiments, Student's t-test). (C) The protein levels of MFF in 40 μg/mL CIP-exposed HTR-8/SVneo cells treated with 10 μM MG132 or 50 μM CQ and co-treated with 10 μM CHX and its relative quantification (n = 3 independent experiments, one-way ANOVA). (D and E) IP assay analysis of the protein levels of MFF-Ub pulled down by MFF in 40 μg/mL CIP-exposed HTR-8/SVneo cells and its relative quantification (n = 3 independent experiments, Student's t-test). (F) MFF IP-MS assay analysis of the proteins that were pulled down by MFF in HTR-8/SVneo cells. (G–K) IP assay analysis of the protein levels of TRIM21 and MFF-Ub pulled down by MFF in HTR-8/SVneo cells with overexpression (Student's t-test) or knockdown (one-way ANOVA) of TRIM21, with MFF and TRIM21 protein levels in cell lysates and their relative quantification (n = 3 independent experiments).

Journal: eBioMedicine

Article Title: Environmental ciprofloxacin triggers pregnancy loss: senescence-driven miscarriage via TRIM21-mediated MFF degradation

doi: 10.1016/j.ebiom.2026.106146

Figure Lengend Snippet: TRIM21 was an E3 ubiquitin ligase of MFF and promoted its ubiquitination degradation. (A and B) The levels of the remained MFF protein in 40 μg/mL CIP-exposed HTR-8/SVneo cells and with 10 μM CHX treatment within 10 h and its relative quantification (n = 3 independent experiments, Student's t-test). (C) The protein levels of MFF in 40 μg/mL CIP-exposed HTR-8/SVneo cells treated with 10 μM MG132 or 50 μM CQ and co-treated with 10 μM CHX and its relative quantification (n = 3 independent experiments, one-way ANOVA). (D and E) IP assay analysis of the protein levels of MFF-Ub pulled down by MFF in 40 μg/mL CIP-exposed HTR-8/SVneo cells and its relative quantification (n = 3 independent experiments, Student's t-test). (F) MFF IP-MS assay analysis of the proteins that were pulled down by MFF in HTR-8/SVneo cells. (G–K) IP assay analysis of the protein levels of TRIM21 and MFF-Ub pulled down by MFF in HTR-8/SVneo cells with overexpression (Student's t-test) or knockdown (one-way ANOVA) of TRIM21, with MFF and TRIM21 protein levels in cell lysates and their relative quantification (n = 3 independent experiments).

Article Snippet: The lysates were incubated with MFF antibody (17090-1-AP, proteintech, dilution 1:200, RRID: AB_2142463 ) or TRIM21 antibody (67136-1-Ig, proteintech, dilution 1:200, RRID: AB_2882435 ) at 4 °C overnight, with equal weight of IgG antibody (ab172730, Abcam, dilution 1:200, RRID: AB_2687931 ) as negative control, and then incubated with Protein A/G magnetic beads (HY–K0202, MedChemExpress) for another 6 h to form bead-immunoprecipitate complex.

Techniques: Ubiquitin Proteomics, Quantitative Proteomics, Protein-Protein interactions, Over Expression, Knockdown

The joint roles of CIP and TRIM21 in MFF ubiquitination degradation. (A) TRIM21 mRNA levels in 0, 1, 10, 20, or 40 μg/mL CIP-exposed HTR-8/SVneo cells (n = 3 independent experiments, one-way ANOVA). (B) TRIM21 protein levels in 0, 1, 10, 20, or 40 μg/mL CIP-exposed HTR-8/SVneo cells and its relative quantification (n = 3 independent experiments, one-way ANOVA). (C and D) IP assay analysis of the protein levels of MFF pulled down by TRIM21 in 40 μg/mL CIP-exposed HTR-8/SVneo cells with MFF and TRIM21 protein levels in cell lysates and its relative quantification (n = 3 independent experiments, Student's t-test). (E–I) IP assay analysis of the protein levels of TRIM21 and MFF-Ub pulled down by MFF in 40 μg/mL CIP-exposed HTR-8/SVneo cells with TRIM21 knockdown, with MFF and TRIM21 protein levels in cell lysates and their relative quantification (n = 3 independent experiments, one-way ANOVA).

Journal: eBioMedicine

Article Title: Environmental ciprofloxacin triggers pregnancy loss: senescence-driven miscarriage via TRIM21-mediated MFF degradation

doi: 10.1016/j.ebiom.2026.106146

Figure Lengend Snippet: The joint roles of CIP and TRIM21 in MFF ubiquitination degradation. (A) TRIM21 mRNA levels in 0, 1, 10, 20, or 40 μg/mL CIP-exposed HTR-8/SVneo cells (n = 3 independent experiments, one-way ANOVA). (B) TRIM21 protein levels in 0, 1, 10, 20, or 40 μg/mL CIP-exposed HTR-8/SVneo cells and its relative quantification (n = 3 independent experiments, one-way ANOVA). (C and D) IP assay analysis of the protein levels of MFF pulled down by TRIM21 in 40 μg/mL CIP-exposed HTR-8/SVneo cells with MFF and TRIM21 protein levels in cell lysates and its relative quantification (n = 3 independent experiments, Student's t-test). (E–I) IP assay analysis of the protein levels of TRIM21 and MFF-Ub pulled down by MFF in 40 μg/mL CIP-exposed HTR-8/SVneo cells with TRIM21 knockdown, with MFF and TRIM21 protein levels in cell lysates and their relative quantification (n = 3 independent experiments, one-way ANOVA).

Article Snippet: The lysates were incubated with MFF antibody (17090-1-AP, proteintech, dilution 1:200, RRID: AB_2142463 ) or TRIM21 antibody (67136-1-Ig, proteintech, dilution 1:200, RRID: AB_2882435 ) at 4 °C overnight, with equal weight of IgG antibody (ab172730, Abcam, dilution 1:200, RRID: AB_2687931 ) as negative control, and then incubated with Protein A/G magnetic beads (HY–K0202, MedChemExpress) for another 6 h to form bead-immunoprecipitate complex.

Techniques: Ubiquitin Proteomics, Quantitative Proteomics, Knockdown

Verification of TRIM21/MFF signalling in human villous tissues. (A and B) TRIM21 protein levels in HC and UM villous tissues and its relative quantification (n = 12, Student's t-test). (C–G) IP assay analysis of the protein levels of TRIM21 and MFF-Ub that were pulled down by MFF in HC and UM villous tissues, with MFF and TRIM21 protein levels in tissue lysates and their relative quantification (each n = 12, Student's t-test). (H) Multivariate logistic regression analysis of forest plot of odds ratio (OR) of TRIM21 protein levels in villous tissues with its 95% confidence interval (95% CI) (n = 12).

Journal: eBioMedicine

Article Title: Environmental ciprofloxacin triggers pregnancy loss: senescence-driven miscarriage via TRIM21-mediated MFF degradation

doi: 10.1016/j.ebiom.2026.106146

Figure Lengend Snippet: Verification of TRIM21/MFF signalling in human villous tissues. (A and B) TRIM21 protein levels in HC and UM villous tissues and its relative quantification (n = 12, Student's t-test). (C–G) IP assay analysis of the protein levels of TRIM21 and MFF-Ub that were pulled down by MFF in HC and UM villous tissues, with MFF and TRIM21 protein levels in tissue lysates and their relative quantification (each n = 12, Student's t-test). (H) Multivariate logistic regression analysis of forest plot of odds ratio (OR) of TRIM21 protein levels in villous tissues with its 95% confidence interval (95% CI) (n = 12).

Article Snippet: The lysates were incubated with MFF antibody (17090-1-AP, proteintech, dilution 1:200, RRID: AB_2142463 ) or TRIM21 antibody (67136-1-Ig, proteintech, dilution 1:200, RRID: AB_2882435 ) at 4 °C overnight, with equal weight of IgG antibody (ab172730, Abcam, dilution 1:200, RRID: AB_2687931 ) as negative control, and then incubated with Protein A/G magnetic beads (HY–K0202, MedChemExpress) for another 6 h to form bead-immunoprecipitate complex.

Techniques: Quantitative Proteomics

Verification of murine Trim21/Mff signalling in CIP-exposed mouse placental tissues. (A) Murine Trim21 mRNA levels in placental tissues of CIP-exposed mice (n = 6, one-way ANOVA). (B and C) Murine Trim21 protein levels in placental tissues of CIP-exposed mice and its relative quantification (n = 6, one-way ANOVA). (D) IP assay analysis of the protein levels of Trim21 and Mff-Ub that were pulled down by Mff in placental tissues of 160 mg/kg/d CIP-exposed mice, with Mff and Trim21 protein levels in tissue lysates (each n = 6). (E) Schematic diagram of CIP-exposed mouse model with Trim21 knockdown. Pregnant mice were treated with saline or 160 mg/kg/d CIP and also treated with 10 mg/kg/3 d AS-Trim 21 (with AS–NC as control). (F and G) Embryo resorption (indicated by red arrows) and the average miscarriage rates in 160 mg/kg/d CIP-exposed mice with Trim21 knockdown (each n = 6, one-way ANOVA). (H) IP assay analysis of the protein levels of Trim21 and Mff-Ub that were pulled down by Mff in 160 mg/kg/d CIP-exposed mice with Trim21 knockdown, with Mff and Trim21 protein levels in tissue lysates (each n = 6). (I) The quantification of positive intensity of SA-β-Galactose staining of placental tissues in 160 mg/kg/d CIP-exposed mice with Trim21 knockdown (each n = 6, one-way ANOVA). (J) The protein levels of p16, p21, p38, and β-gal in placental tissues of 160 mg/kg/d CIP-exposed mice with Trim21 knockdown (each n = 6). (K) The relative mtDNA copy number in placental tissues of 160 mg/kg/d CIP-exposed mice with Trim21 knockdown (n = 6, one-way ANOVA). (L) The NAD + /NADH ratios in placental tissues of 160 mg/kg/d CIP-exposed mice with Trim21 knockdown (n = 6, one-way ANOVA). (M) The MMP levels in placental tissues of 160 mg/kg/d CIP-exposed mice with Trim21 knockdown (n = 6, one-way ANOVA).

Journal: eBioMedicine

Article Title: Environmental ciprofloxacin triggers pregnancy loss: senescence-driven miscarriage via TRIM21-mediated MFF degradation

doi: 10.1016/j.ebiom.2026.106146

Figure Lengend Snippet: Verification of murine Trim21/Mff signalling in CIP-exposed mouse placental tissues. (A) Murine Trim21 mRNA levels in placental tissues of CIP-exposed mice (n = 6, one-way ANOVA). (B and C) Murine Trim21 protein levels in placental tissues of CIP-exposed mice and its relative quantification (n = 6, one-way ANOVA). (D) IP assay analysis of the protein levels of Trim21 and Mff-Ub that were pulled down by Mff in placental tissues of 160 mg/kg/d CIP-exposed mice, with Mff and Trim21 protein levels in tissue lysates (each n = 6). (E) Schematic diagram of CIP-exposed mouse model with Trim21 knockdown. Pregnant mice were treated with saline or 160 mg/kg/d CIP and also treated with 10 mg/kg/3 d AS-Trim 21 (with AS–NC as control). (F and G) Embryo resorption (indicated by red arrows) and the average miscarriage rates in 160 mg/kg/d CIP-exposed mice with Trim21 knockdown (each n = 6, one-way ANOVA). (H) IP assay analysis of the protein levels of Trim21 and Mff-Ub that were pulled down by Mff in 160 mg/kg/d CIP-exposed mice with Trim21 knockdown, with Mff and Trim21 protein levels in tissue lysates (each n = 6). (I) The quantification of positive intensity of SA-β-Galactose staining of placental tissues in 160 mg/kg/d CIP-exposed mice with Trim21 knockdown (each n = 6, one-way ANOVA). (J) The protein levels of p16, p21, p38, and β-gal in placental tissues of 160 mg/kg/d CIP-exposed mice with Trim21 knockdown (each n = 6). (K) The relative mtDNA copy number in placental tissues of 160 mg/kg/d CIP-exposed mice with Trim21 knockdown (n = 6, one-way ANOVA). (L) The NAD + /NADH ratios in placental tissues of 160 mg/kg/d CIP-exposed mice with Trim21 knockdown (n = 6, one-way ANOVA). (M) The MMP levels in placental tissues of 160 mg/kg/d CIP-exposed mice with Trim21 knockdown (n = 6, one-way ANOVA).

Article Snippet: The lysates were incubated with MFF antibody (17090-1-AP, proteintech, dilution 1:200, RRID: AB_2142463 ) or TRIM21 antibody (67136-1-Ig, proteintech, dilution 1:200, RRID: AB_2882435 ) at 4 °C overnight, with equal weight of IgG antibody (ab172730, Abcam, dilution 1:200, RRID: AB_2687931 ) as negative control, and then incubated with Protein A/G magnetic beads (HY–K0202, MedChemExpress) for another 6 h to form bead-immunoprecipitate complex.

Techniques: Quantitative Proteomics, Knockdown, Saline, Control, Staining

Miscarriage treatment. (A) Schematic diagram of CIP-exposed mouse model with F + Q treatment. Pregnant mice were treated with saline or 160 mg/kg/d CIP and also treated with 50 mg/kg/2 d F and 10 mg/kg/2 d Q. (B–D) The protein levels of Trim21 and Mff in placental tissues of 160 mg/kg/d CIP-exposed mice with F + Q treatment and their relative quantification (each n = 6, Student's t-test). (E and F) Embryo resorption (indicated by red arrows) and the average miscarriage rates in 160 mg/kg/d CIP-exposed mice with F + Q treatment (each n = 6, Student's t-test). (G) IP assay analysis of the protein levels of Trim21 and Mff-Ub that were pulled down by Mff in 160 mg/kg/d CIP-exposed mice with F + Q treatment, with Mff and Trim21 protein levels in tissue lysates (each n = 6). (H) The quantification of positive intensity of SA-β-Galactose staining of placental tissues of 160 mg/kg/d CIP-exposed mice with F + Q treatment (each n = 6, Student's t-test). (I) The protein levels of p16, p21, p38, and β-gal in placental tissues of 160 mg/kg/d CIP-exposed mice with F + Q treatment (each n = 6). (J) The relative mtDNA copy number in placental tissues of 160 mg/kg/d CIP-exposed mice with F + Q treatment (n = 6, Student's t-test). (K) The NAD + /NADH ratios in placental tissues of 160 mg/kg/d CIP-exposed mice with F + Q treatment (n = 6, Student's t-test). (L) The MMP levels in placental tissues of 160 mg/kg/d CIP-exposed mice with F + Q treatment (n = 6, Student's t-test). (M) The proposed mechanisms.

Journal: eBioMedicine

Article Title: Environmental ciprofloxacin triggers pregnancy loss: senescence-driven miscarriage via TRIM21-mediated MFF degradation

doi: 10.1016/j.ebiom.2026.106146

Figure Lengend Snippet: Miscarriage treatment. (A) Schematic diagram of CIP-exposed mouse model with F + Q treatment. Pregnant mice were treated with saline or 160 mg/kg/d CIP and also treated with 50 mg/kg/2 d F and 10 mg/kg/2 d Q. (B–D) The protein levels of Trim21 and Mff in placental tissues of 160 mg/kg/d CIP-exposed mice with F + Q treatment and their relative quantification (each n = 6, Student's t-test). (E and F) Embryo resorption (indicated by red arrows) and the average miscarriage rates in 160 mg/kg/d CIP-exposed mice with F + Q treatment (each n = 6, Student's t-test). (G) IP assay analysis of the protein levels of Trim21 and Mff-Ub that were pulled down by Mff in 160 mg/kg/d CIP-exposed mice with F + Q treatment, with Mff and Trim21 protein levels in tissue lysates (each n = 6). (H) The quantification of positive intensity of SA-β-Galactose staining of placental tissues of 160 mg/kg/d CIP-exposed mice with F + Q treatment (each n = 6, Student's t-test). (I) The protein levels of p16, p21, p38, and β-gal in placental tissues of 160 mg/kg/d CIP-exposed mice with F + Q treatment (each n = 6). (J) The relative mtDNA copy number in placental tissues of 160 mg/kg/d CIP-exposed mice with F + Q treatment (n = 6, Student's t-test). (K) The NAD + /NADH ratios in placental tissues of 160 mg/kg/d CIP-exposed mice with F + Q treatment (n = 6, Student's t-test). (L) The MMP levels in placental tissues of 160 mg/kg/d CIP-exposed mice with F + Q treatment (n = 6, Student's t-test). (M) The proposed mechanisms.

Article Snippet: The lysates were incubated with MFF antibody (17090-1-AP, proteintech, dilution 1:200, RRID: AB_2142463 ) or TRIM21 antibody (67136-1-Ig, proteintech, dilution 1:200, RRID: AB_2882435 ) at 4 °C overnight, with equal weight of IgG antibody (ab172730, Abcam, dilution 1:200, RRID: AB_2687931 ) as negative control, and then incubated with Protein A/G magnetic beads (HY–K0202, MedChemExpress) for another 6 h to form bead-immunoprecipitate complex.

Techniques: Saline, Quantitative Proteomics, Staining