mff Search Results


93
Santa Cruz Biotechnology anti mff santa cruz
Anti Mff Santa Cruz, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene mmfcrn sirna
FIGURE 3 FcRn mRNA and differentiation marker expression after FcRn <t>siRNA</t> transfection. M-Mf and GM-Mf were transfected with FcRn siRNA on day 3 for 24 h. (A) Transfection efficiency was assessed by RT-qPCR. Relative expression of FCGRT was normalized to that of actin and GAPDH. Data are relative FcRn levels to siRNA control (mean ± SD, N=7). (B) Flow cytometry of CD163, CD14, CD206 and CD16 expression after transfection in M-Mf and GM-Mf. Data are normalized MFI fold change to siRNA control (mean ± SD, N=7). M-Mf, M-CSF–induced macrophages; GM-Mf, GM-CSF–induced macrophages; MFI, mean fluorescence intensity; ns, not significant. ns = not significant and *p ≤0.05.
Mmfcrn Sirna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc h4 aa 1 30
FIGURE 3 FcRn mRNA and differentiation marker expression after FcRn <t>siRNA</t> transfection. M-Mf and GM-Mf were transfected with FcRn siRNA on day 3 for 24 h. (A) Transfection efficiency was assessed by RT-qPCR. Relative expression of FCGRT was normalized to that of actin and GAPDH. Data are relative FcRn levels to siRNA control (mean ± SD, N=7). (B) Flow cytometry of CD163, CD14, CD206 and CD16 expression after transfection in M-Mf and GM-Mf. Data are normalized MFI fold change to siRNA control (mean ± SD, N=7). M-Mf, M-CSF–induced macrophages; GM-Mf, GM-CSF–induced macrophages; MFI, mean fluorescence intensity; ns, not significant. ns = not significant and *p ≤0.05.
H4 Aa 1 30, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Atlas Antibodies mff
FIGURE 3 FcRn mRNA and differentiation marker expression after FcRn <t>siRNA</t> transfection. M-Mf and GM-Mf were transfected with FcRn siRNA on day 3 for 24 h. (A) Transfection efficiency was assessed by RT-qPCR. Relative expression of FCGRT was normalized to that of actin and GAPDH. Data are relative FcRn levels to siRNA control (mean ± SD, N=7). (B) Flow cytometry of CD163, CD14, CD206 and CD16 expression after transfection in M-Mf and GM-Mf. Data are normalized MFI fold change to siRNA control (mean ± SD, N=7). M-Mf, M-CSF–induced macrophages; GM-Mf, GM-CSF–induced macrophages; MFI, mean fluorescence intensity; ns, not significant. ns = not significant and *p ≤0.05.
Mff, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Novus Biologicals mitochondrial fission factor mff
FIGURE 3 FcRn mRNA and differentiation marker expression after FcRn <t>siRNA</t> transfection. M-Mf and GM-Mf were transfected with FcRn siRNA on day 3 for 24 h. (A) Transfection efficiency was assessed by RT-qPCR. Relative expression of FCGRT was normalized to that of actin and GAPDH. Data are relative FcRn levels to siRNA control (mean ± SD, N=7). (B) Flow cytometry of CD163, CD14, CD206 and CD16 expression after transfection in M-Mf and GM-Mf. Data are normalized MFI fold change to siRNA control (mean ± SD, N=7). M-Mf, M-CSF–induced macrophages; GM-Mf, GM-CSF–induced macrophages; MFI, mean fluorescence intensity; ns, not significant. ns = not significant and *p ≤0.05.
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Thermo Fisher gene exp mff mm01273401 m1
FIGURE 3 FcRn mRNA and differentiation marker expression after FcRn <t>siRNA</t> transfection. M-Mf and GM-Mf were transfected with FcRn siRNA on day 3 for 24 h. (A) Transfection efficiency was assessed by RT-qPCR. Relative expression of FCGRT was normalized to that of actin and GAPDH. Data are relative FcRn levels to siRNA control (mean ± SD, N=7). (B) Flow cytometry of CD163, CD14, CD206 and CD16 expression after transfection in M-Mf and GM-Mf. Data are normalized MFI fold change to siRNA control (mean ± SD, N=7). M-Mf, M-CSF–induced macrophages; GM-Mf, GM-CSF–induced macrophages; MFI, mean fluorescence intensity; ns, not significant. ns = not significant and *p ≤0.05.
Gene Exp Mff Mm01273401 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 3 FcRn mRNA and differentiation marker expression after FcRn siRNA transfection. M-Mf and GM-Mf were transfected with FcRn siRNA on day 3 for 24 h. (A) Transfection efficiency was assessed by RT-qPCR. Relative expression of FCGRT was normalized to that of actin and GAPDH. Data are relative FcRn levels to siRNA control (mean ± SD, N=7). (B) Flow cytometry of CD163, CD14, CD206 and CD16 expression after transfection in M-Mf and GM-Mf. Data are normalized MFI fold change to siRNA control (mean ± SD, N=7). M-Mf, M-CSF–induced macrophages; GM-Mf, GM-CSF–induced macrophages; MFI, mean fluorescence intensity; ns, not significant. ns = not significant and *p ≤0.05.

Journal: Frontiers in immunology

Article Title: The neonatal Fc receptor expression during macrophage differentiation is related to autophagy.

doi: 10.3389/fimmu.2022.1054425

Figure Lengend Snippet: FIGURE 3 FcRn mRNA and differentiation marker expression after FcRn siRNA transfection. M-Mf and GM-Mf were transfected with FcRn siRNA on day 3 for 24 h. (A) Transfection efficiency was assessed by RT-qPCR. Relative expression of FCGRT was normalized to that of actin and GAPDH. Data are relative FcRn levels to siRNA control (mean ± SD, N=7). (B) Flow cytometry of CD163, CD14, CD206 and CD16 expression after transfection in M-Mf and GM-Mf. Data are normalized MFI fold change to siRNA control (mean ± SD, N=7). M-Mf, M-CSF–induced macrophages; GM-Mf, GM-CSF–induced macrophages; MFI, mean fluorescence intensity; ns, not significant. ns = not significant and *p ≤0.05.

Article Snippet: An amount of 20 mMFcRn siRNA (Origene, USA) or siRNA control (Eurogentec, Belgium) was diluted in 200 ml Opti-MEM (Gibco, France) containing 1 ml Lipofectamine RNAiMAX (Thermo Fisher Scientific, USA).

Techniques: Marker, Expressing, Transfection, Quantitative RT-PCR, Control, Flow Cytometry