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methylpyruvate  (ATCC)


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    ATCC methylpyruvate
    Methylpyruvate, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/methylpyruvate/product/ATCC
    Average 90 stars, based on 2 article reviews
    methylpyruvate - by Bioz Stars, 2026-06
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    a , qRT-PCR analyses of pro-autophagic genes in old HSCs (n = 3–5). Results are expressed as log2 fold expression compared to young HSCs (set to 0). b , Apoptosis levels in cultured young and old HSCs (n = 3). Results are expressed as percent caspase activation compared to +cytokines young HSCs. Hatching indicates –cytokines conditions. c , 2-NBD glucose uptake in cultured young and old HSCs (n = 3). d , Percent colony formation in young and old WT HSCs cultured for 32h ± BafA and <t>methylpyruvate</t> (M-Pyr) (n = 3). Colonies were counted at day 10 and normalized to -BafA conditions. Data are means ± s.d. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
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    a , qRT-PCR analyses of pro-autophagic genes in old HSCs (n = 3–5). Results are expressed as log2 fold expression compared to young HSCs (set to 0). b , Apoptosis levels in cultured young and old HSCs (n = 3). Results are expressed as percent caspase activation compared to +cytokines young HSCs. Hatching indicates –cytokines conditions. c , 2-NBD glucose uptake in cultured young and old HSCs (n = 3). d , Percent colony formation in young and old WT HSCs cultured for 32h ± BafA and <t>methylpyruvate</t> (M-Pyr) (n = 3). Colonies were counted at day 10 and normalized to -BafA conditions. Data are means ± s.d. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
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    a , qRT-PCR analyses of pro-autophagic genes in old HSCs (n = 3–5). Results are expressed as log2 fold expression compared to young HSCs (set to 0). b , Apoptosis levels in cultured young and old HSCs (n = 3). Results are expressed as percent caspase activation compared to +cytokines young HSCs. Hatching indicates –cytokines conditions. c , 2-NBD glucose uptake in cultured young and old HSCs (n = 3). d , Percent colony formation in young and old WT HSCs cultured for 32h ± BafA and methylpyruvate (M-Pyr) (n = 3). Colonies were counted at day 10 and normalized to -BafA conditions. Data are means ± s.d. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.

    Journal: Nature

    Article Title: FoxO3a Directs a Protective Autophagy Program in Hematopoietic Stem Cells

    doi: 10.1038/nature11895

    Figure Lengend Snippet: a , qRT-PCR analyses of pro-autophagic genes in old HSCs (n = 3–5). Results are expressed as log2 fold expression compared to young HSCs (set to 0). b , Apoptosis levels in cultured young and old HSCs (n = 3). Results are expressed as percent caspase activation compared to +cytokines young HSCs. Hatching indicates –cytokines conditions. c , 2-NBD glucose uptake in cultured young and old HSCs (n = 3). d , Percent colony formation in young and old WT HSCs cultured for 32h ± BafA and methylpyruvate (M-Pyr) (n = 3). Colonies were counted at day 10 and normalized to -BafA conditions. Data are means ± s.d. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.

    Article Snippet: Bafilomycin A (BafA, 5 nM, Sigma), methylpyruvate (M-Pyr, 10 mM, Sigma), rapamycin (Rapa, 20 μM, EMD Millipore) or appropriate vehicles (DMSO for BafA and Rapa) were added to the media as indicated.

    Techniques: Quantitative RT-PCR, Expressing, Cell Culture, Activation Assay