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ubiquitous cytomegalovirus cmv promoter sequence  (OriGene)


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    OriGene ubiquitous cytomegalovirus cmv promoter sequence
    Ubiquitous Cytomegalovirus Cmv Promoter Sequence, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/matriptase/Matriptase+2+(TMPRSS6)+(NM_153609)+Human+Untagged+Clone/us12503701-1418-37-32
    Average 93 stars, based on 1 article reviews
    ubiquitous cytomegalovirus cmv promoter sequence - by Bioz Stars, 2026-09
    93/100 stars

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    other:

    Article Title: Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways
    Article Snippet: Proteins were transferred to polyvinylidene fluoride membranes and detected by specific primary antibodies and HRP-conjugated secondary antibodies using SuperSignal West Femto or Dura chemiluminescent Western blotting substrates (Thermo Fisher Scientific).

    Clone Assay:

    Article Title: Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways
    Article Snippet: .. The cDNA of the HA gene of B/Malaysia/2506/2004 was cloned from viral RNA by RT-PCR using HA-specific primers and subsequently subcloned into pCAGGS expression plasmid using EcoRI and NotI restriction sites. pCMV6-Entry expression plasmids encoding murine proteases with a C-terminal Myc-DDK tag were obtained from OriGene Technologies: CFB (MR210521), HGFA (MR216475), hepsin (MR219750), LTF (MR210170), tPA (MR208868), uPA (MR225747), tryptase ϵ (MR204321), NSP4 (MR217041), prostasin (MR223227), matriptase (MR222240), TMPRSS4 (MR206946), and TMPRSS13 (MR220731). pCAGGS plasmids encoding human or murine TMPRSS2 with C-terminal FLAG epitope have been described previously ( 6 , 15 ). pCAGGS encoding human prostasin was described previously ( 31 ). pcDNA6.2/C-emGFP-HPN encoding human hepsin (Human ORFeome Collaboration, Clone ID: 100004749) was generously provided by the Juha Klefström laboratory (University of Helsinki). .. The cDNA encoding tryptase ϵ_DDDDK mutant with substitution of Arg-49 by the enterokinase cleavage site motif DDDDK was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) based on the cDNA sequence of the murine Prss22 gene (OriGene Technologies, MR204321) and was cloned into the pCMV6-Entry plasmid using HindIII and NotI restriction sites.

    Article Title: Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways
    Article Snippet: .. The cDNA of the HA gene of B/Malaysia/2506/2004 was cloned from viral RNA by RT-PCR using HA-specific primers and subsequently subcloned into pCAGGS expression plasmid using EcoRI and NotI restriction sites. pCMV6-Entry expression plasmids encoding murine proteases with Cterminal Myc-DDK tag were obtained from OriGene Technologies: CFB (MR210521), HGFA (MR216475), Hepsin (MR219750), LTF (MR210170), tPA (MR208868), uPA (MR225747), tryptase ɛ (MR204321), NSP4 (MR217041), prostasin (MR223227), matriptase (MR222240), TMPRSS4 (MR206946), and TMPRSS13 (MR220731). pCAGGS plasmids encoding human or murine TMPRSS2 with Cterminal FLAG epitope have been described previously (6, 15). pCAGGS encoding human prostasin was described in (31). pcDNA6.2/CemGFP-HPN encoding human hepsin (Human ORFeome Collaboration, Clone Id: 100004749) was generously provided by the Juha Klefström laboratory (University of Helsinki). .. The cDNA encoding tryptase ε_DDDDK mutant with substitution of R49 by the enterokinase cleavage site motif DDDDK was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) based on the cDNA sequence of the murine Prss22 gene (OriGene Technologies, MR204321) and was cloned into pCMV6-Entry plasmid using HindIII and NotI restriction sites.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways
    Article Snippet: .. The cDNA of the HA gene of B/Malaysia/2506/2004 was cloned from viral RNA by RT-PCR using HA-specific primers and subsequently subcloned into pCAGGS expression plasmid using EcoRI and NotI restriction sites. pCMV6-Entry expression plasmids encoding murine proteases with a C-terminal Myc-DDK tag were obtained from OriGene Technologies: CFB (MR210521), HGFA (MR216475), hepsin (MR219750), LTF (MR210170), tPA (MR208868), uPA (MR225747), tryptase ϵ (MR204321), NSP4 (MR217041), prostasin (MR223227), matriptase (MR222240), TMPRSS4 (MR206946), and TMPRSS13 (MR220731). pCAGGS plasmids encoding human or murine TMPRSS2 with C-terminal FLAG epitope have been described previously ( 6 , 15 ). pCAGGS encoding human prostasin was described previously ( 31 ). pcDNA6.2/C-emGFP-HPN encoding human hepsin (Human ORFeome Collaboration, Clone ID: 100004749) was generously provided by the Juha Klefström laboratory (University of Helsinki). .. The cDNA encoding tryptase ϵ_DDDDK mutant with substitution of Arg-49 by the enterokinase cleavage site motif DDDDK was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) based on the cDNA sequence of the murine Prss22 gene (OriGene Technologies, MR204321) and was cloned into the pCMV6-Entry plasmid using HindIII and NotI restriction sites.

    Article Title: Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways
    Article Snippet: .. The cDNA of the HA gene of B/Malaysia/2506/2004 was cloned from viral RNA by RT-PCR using HA-specific primers and subsequently subcloned into pCAGGS expression plasmid using EcoRI and NotI restriction sites. pCMV6-Entry expression plasmids encoding murine proteases with Cterminal Myc-DDK tag were obtained from OriGene Technologies: CFB (MR210521), HGFA (MR216475), Hepsin (MR219750), LTF (MR210170), tPA (MR208868), uPA (MR225747), tryptase ɛ (MR204321), NSP4 (MR217041), prostasin (MR223227), matriptase (MR222240), TMPRSS4 (MR206946), and TMPRSS13 (MR220731). pCAGGS plasmids encoding human or murine TMPRSS2 with Cterminal FLAG epitope have been described previously (6, 15). pCAGGS encoding human prostasin was described in (31). pcDNA6.2/CemGFP-HPN encoding human hepsin (Human ORFeome Collaboration, Clone Id: 100004749) was generously provided by the Juha Klefström laboratory (University of Helsinki). .. The cDNA encoding tryptase ε_DDDDK mutant with substitution of R49 by the enterokinase cleavage site motif DDDDK was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) based on the cDNA sequence of the murine Prss22 gene (OriGene Technologies, MR204321) and was cloned into pCMV6-Entry plasmid using HindIII and NotI restriction sites.

    Expressing:

    Article Title: Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways
    Article Snippet: .. The cDNA of the HA gene of B/Malaysia/2506/2004 was cloned from viral RNA by RT-PCR using HA-specific primers and subsequently subcloned into pCAGGS expression plasmid using EcoRI and NotI restriction sites. pCMV6-Entry expression plasmids encoding murine proteases with a C-terminal Myc-DDK tag were obtained from OriGene Technologies: CFB (MR210521), HGFA (MR216475), hepsin (MR219750), LTF (MR210170), tPA (MR208868), uPA (MR225747), tryptase ϵ (MR204321), NSP4 (MR217041), prostasin (MR223227), matriptase (MR222240), TMPRSS4 (MR206946), and TMPRSS13 (MR220731). pCAGGS plasmids encoding human or murine TMPRSS2 with C-terminal FLAG epitope have been described previously ( 6 , 15 ). pCAGGS encoding human prostasin was described previously ( 31 ). pcDNA6.2/C-emGFP-HPN encoding human hepsin (Human ORFeome Collaboration, Clone ID: 100004749) was generously provided by the Juha Klefström laboratory (University of Helsinki). .. The cDNA encoding tryptase ϵ_DDDDK mutant with substitution of Arg-49 by the enterokinase cleavage site motif DDDDK was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) based on the cDNA sequence of the murine Prss22 gene (OriGene Technologies, MR204321) and was cloned into the pCMV6-Entry plasmid using HindIII and NotI restriction sites.

    Article Title: Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways
    Article Snippet: .. The cDNA of the HA gene of B/Malaysia/2506/2004 was cloned from viral RNA by RT-PCR using HA-specific primers and subsequently subcloned into pCAGGS expression plasmid using EcoRI and NotI restriction sites. pCMV6-Entry expression plasmids encoding murine proteases with Cterminal Myc-DDK tag were obtained from OriGene Technologies: CFB (MR210521), HGFA (MR216475), Hepsin (MR219750), LTF (MR210170), tPA (MR208868), uPA (MR225747), tryptase ɛ (MR204321), NSP4 (MR217041), prostasin (MR223227), matriptase (MR222240), TMPRSS4 (MR206946), and TMPRSS13 (MR220731). pCAGGS plasmids encoding human or murine TMPRSS2 with Cterminal FLAG epitope have been described previously (6, 15). pCAGGS encoding human prostasin was described in (31). pcDNA6.2/CemGFP-HPN encoding human hepsin (Human ORFeome Collaboration, Clone Id: 100004749) was generously provided by the Juha Klefström laboratory (University of Helsinki). .. The cDNA encoding tryptase ε_DDDDK mutant with substitution of R49 by the enterokinase cleavage site motif DDDDK was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) based on the cDNA sequence of the murine Prss22 gene (OriGene Technologies, MR204321) and was cloned into pCMV6-Entry plasmid using HindIII and NotI restriction sites.

    Plasmid Preparation:

    Article Title: Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways
    Article Snippet: .. The cDNA of the HA gene of B/Malaysia/2506/2004 was cloned from viral RNA by RT-PCR using HA-specific primers and subsequently subcloned into pCAGGS expression plasmid using EcoRI and NotI restriction sites. pCMV6-Entry expression plasmids encoding murine proteases with a C-terminal Myc-DDK tag were obtained from OriGene Technologies: CFB (MR210521), HGFA (MR216475), hepsin (MR219750), LTF (MR210170), tPA (MR208868), uPA (MR225747), tryptase ϵ (MR204321), NSP4 (MR217041), prostasin (MR223227), matriptase (MR222240), TMPRSS4 (MR206946), and TMPRSS13 (MR220731). pCAGGS plasmids encoding human or murine TMPRSS2 with C-terminal FLAG epitope have been described previously ( 6 , 15 ). pCAGGS encoding human prostasin was described previously ( 31 ). pcDNA6.2/C-emGFP-HPN encoding human hepsin (Human ORFeome Collaboration, Clone ID: 100004749) was generously provided by the Juha Klefström laboratory (University of Helsinki). .. The cDNA encoding tryptase ϵ_DDDDK mutant with substitution of Arg-49 by the enterokinase cleavage site motif DDDDK was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) based on the cDNA sequence of the murine Prss22 gene (OriGene Technologies, MR204321) and was cloned into the pCMV6-Entry plasmid using HindIII and NotI restriction sites.

    Article Title: Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways
    Article Snippet: .. The cDNA of the HA gene of B/Malaysia/2506/2004 was cloned from viral RNA by RT-PCR using HA-specific primers and subsequently subcloned into pCAGGS expression plasmid using EcoRI and NotI restriction sites. pCMV6-Entry expression plasmids encoding murine proteases with Cterminal Myc-DDK tag were obtained from OriGene Technologies: CFB (MR210521), HGFA (MR216475), Hepsin (MR219750), LTF (MR210170), tPA (MR208868), uPA (MR225747), tryptase ɛ (MR204321), NSP4 (MR217041), prostasin (MR223227), matriptase (MR222240), TMPRSS4 (MR206946), and TMPRSS13 (MR220731). pCAGGS plasmids encoding human or murine TMPRSS2 with Cterminal FLAG epitope have been described previously (6, 15). pCAGGS encoding human prostasin was described in (31). pcDNA6.2/CemGFP-HPN encoding human hepsin (Human ORFeome Collaboration, Clone Id: 100004749) was generously provided by the Juha Klefström laboratory (University of Helsinki). .. The cDNA encoding tryptase ε_DDDDK mutant with substitution of R49 by the enterokinase cleavage site motif DDDDK was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) based on the cDNA sequence of the murine Prss22 gene (OriGene Technologies, MR204321) and was cloned into pCMV6-Entry plasmid using HindIII and NotI restriction sites.

    FLAG-tag:

    Article Title: Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways
    Article Snippet: .. The cDNA of the HA gene of B/Malaysia/2506/2004 was cloned from viral RNA by RT-PCR using HA-specific primers and subsequently subcloned into pCAGGS expression plasmid using EcoRI and NotI restriction sites. pCMV6-Entry expression plasmids encoding murine proteases with a C-terminal Myc-DDK tag were obtained from OriGene Technologies: CFB (MR210521), HGFA (MR216475), hepsin (MR219750), LTF (MR210170), tPA (MR208868), uPA (MR225747), tryptase ϵ (MR204321), NSP4 (MR217041), prostasin (MR223227), matriptase (MR222240), TMPRSS4 (MR206946), and TMPRSS13 (MR220731). pCAGGS plasmids encoding human or murine TMPRSS2 with C-terminal FLAG epitope have been described previously ( 6 , 15 ). pCAGGS encoding human prostasin was described previously ( 31 ). pcDNA6.2/C-emGFP-HPN encoding human hepsin (Human ORFeome Collaboration, Clone ID: 100004749) was generously provided by the Juha Klefström laboratory (University of Helsinki). .. The cDNA encoding tryptase ϵ_DDDDK mutant with substitution of Arg-49 by the enterokinase cleavage site motif DDDDK was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) based on the cDNA sequence of the murine Prss22 gene (OriGene Technologies, MR204321) and was cloned into the pCMV6-Entry plasmid using HindIII and NotI restriction sites.

    Article Title: Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways
    Article Snippet: .. The cDNA of the HA gene of B/Malaysia/2506/2004 was cloned from viral RNA by RT-PCR using HA-specific primers and subsequently subcloned into pCAGGS expression plasmid using EcoRI and NotI restriction sites. pCMV6-Entry expression plasmids encoding murine proteases with Cterminal Myc-DDK tag were obtained from OriGene Technologies: CFB (MR210521), HGFA (MR216475), Hepsin (MR219750), LTF (MR210170), tPA (MR208868), uPA (MR225747), tryptase ɛ (MR204321), NSP4 (MR217041), prostasin (MR223227), matriptase (MR222240), TMPRSS4 (MR206946), and TMPRSS13 (MR220731). pCAGGS plasmids encoding human or murine TMPRSS2 with Cterminal FLAG epitope have been described previously (6, 15). pCAGGS encoding human prostasin was described in (31). pcDNA6.2/CemGFP-HPN encoding human hepsin (Human ORFeome Collaboration, Clone Id: 100004749) was generously provided by the Juha Klefström laboratory (University of Helsinki). .. The cDNA encoding tryptase ε_DDDDK mutant with substitution of R49 by the enterokinase cleavage site motif DDDDK was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) based on the cDNA sequence of the murine Prss22 gene (OriGene Technologies, MR204321) and was cloned into pCMV6-Entry plasmid using HindIII and NotI restriction sites.



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    OriGene pcmv6 matriptase
    Structures of the masking reagent consist of: 1) a maleimide for conjugation, 2) a <t>matriptase‐cleavable</t> peptide (highlighted in purple), and 3) PEG to create steric hindrance, preventing the antibody from binding to the target.
    Pcmv6 Matriptase, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/matriptase/ST14+(NM_021978)+Human+Tagged+ORF+Clone/pmc12496188-48-0-2
    Average 93 stars, based on 1 article reviews
    pcmv6 matriptase - by Bioz Stars, 2026-09
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    matriptase 2 inhibitor  (Sanofi)
    86
    Sanofi matriptase 2 inhibitor
    Structures of the masking reagent consist of: 1) a maleimide for conjugation, 2) a <t>matriptase‐cleavable</t> peptide (highlighted in purple), and 3) PEG to create steric hindrance, preventing the antibody from binding to the target.
    Matriptase 2 Inhibitor, supplied by Sanofi, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/matriptase/2+inhibitor+matriptase/sec_filing____1479615_slash_000095012321003321_slash_sln___f1-2255-12-24
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    Image Search Results


    Colonic PAR2 activation by 2F elicits LSN responses that are mediated by endosomal internalization, PKA and PKC. (A) Experimental framework for LSN recordings showing a cannulated colon in a recording chamber with the LSN aspirated into a suction electrode. (B) Representative action potentials recorded from LSN afferent fiber prestimulation. (C and D) 100 µM 2F application elicits LSN responses illustrated by (C) action potential traces prestimulation and 15 minutes poststimulation and (D) change in LSN firing rate over time after stimulation via the luminal inflow (vertical dotted line). 2F-stimulation increased LSN firing (E and F). Timeline and peak change in firing rate after 100 µM 2F application (vertical dotted line) in tissue pretreated with (E) 50 µM PitStop2 (PS2) inhibitor for endosomal internalization or negative control PitNot2 (PN2) and (F) 100 µM H-89 dihydrochloride (H-89) and bisindolylmaleimide (GFX), PKA and PKC inhibitors or DMSO vehicle. (E) PS2 as well as separate and simultaneous pretreatment with H-89 and GFX reduced the peak response to 2F. (D and E) Independent samples t test comparing the peak change in firing rate (N = 5–9). (F) One-way ANOVA with post-hoc FDR-corrected independent samples t test (N = 5–6). * P < 0.05, ** P < 0.01, *** P < 0.001 **** P < 0.0001. Data are presented as mean ± SD. LSN, lumbar splanchnic nerve; PAR2, protease-activated receptor 2; PKA, protein kinase A; PKC, protein kinase C.

    Journal: Pain Reports

    Article Title: Monoclonal antibody inhibition of PAR2 reduces phenotype severity and pain in murine inflammatory bowel disease

    doi: 10.1097/PR9.0000000000001446

    Figure Lengend Snippet: Colonic PAR2 activation by 2F elicits LSN responses that are mediated by endosomal internalization, PKA and PKC. (A) Experimental framework for LSN recordings showing a cannulated colon in a recording chamber with the LSN aspirated into a suction electrode. (B) Representative action potentials recorded from LSN afferent fiber prestimulation. (C and D) 100 µM 2F application elicits LSN responses illustrated by (C) action potential traces prestimulation and 15 minutes poststimulation and (D) change in LSN firing rate over time after stimulation via the luminal inflow (vertical dotted line). 2F-stimulation increased LSN firing (E and F). Timeline and peak change in firing rate after 100 µM 2F application (vertical dotted line) in tissue pretreated with (E) 50 µM PitStop2 (PS2) inhibitor for endosomal internalization or negative control PitNot2 (PN2) and (F) 100 µM H-89 dihydrochloride (H-89) and bisindolylmaleimide (GFX), PKA and PKC inhibitors or DMSO vehicle. (E) PS2 as well as separate and simultaneous pretreatment with H-89 and GFX reduced the peak response to 2F. (D and E) Independent samples t test comparing the peak change in firing rate (N = 5–9). (F) One-way ANOVA with post-hoc FDR-corrected independent samples t test (N = 5–6). * P < 0.05, ** P < 0.01, *** P < 0.001 **** P < 0.0001. Data are presented as mean ± SD. LSN, lumbar splanchnic nerve; PAR2, protease-activated receptor 2; PKA, protein kinase A; PKC, protein kinase C.

    Article Snippet: When measuring the inhibitory concentration 50 (IC50), cells received PAR650097 mIgG or hIgG or isotype control antibodies, for 60 minutes at room temperature before addition of PAR2 activating matriptase (3946-SEB-010, R&D Systems, Abingdon, United Kingdom).

    Techniques: Activation Assay, Negative Control

    PAR2 activation in the colon by 2F sensitizes LSN responses to mechanical and chemical stimulation of the colon via endosomal internalization, PKA and PKC. LSN action potential firing after mechanical distention: gradually increasing intraluminal pressure from 0 to 80 mm Hg, and chemical application: 1 mM cinnamaldehyde and 1 µM capsaicin are illustrated in (A). (B) Responses to distention were quantified through the change in firing rate over increasing discrete pressure values. (C and D) Responses to chemical stimuli were quantified through the change in firing rate over time after application (vertical dotted line). 100 µM 2F stimulation, but not vehicle control, elicited sensitization of the LSN to (B) distention, (C) cinnamaldehyde, and (D) capsaicin. Pretreatment with PS2 before 2F stimulation reduced subsequent LSN responses to distention, cinnamaldehyde, and capsaicin compared with PN2. Pretreatment with H-89 and GFX, applied either simultaneously or individually, also reduced subsequent peak LSN responses to distention, cinnamaldehyde, and capsaicin compared with DMSO vehicle. (B–D) Independent samples t tests and one-way ANOVA with post-hoc FDR-corrected independent samples t test (N = 5–9). * P < 0.05, ** P < 0.01, **** P < 0.0001. Data are presented as means ± SD. LSN, lumbar splanchnic nerve; PAR2, protease-activated receptor 2; PKA, protein kinase A; PKC, protein kinase C.

    Journal: Pain Reports

    Article Title: Monoclonal antibody inhibition of PAR2 reduces phenotype severity and pain in murine inflammatory bowel disease

    doi: 10.1097/PR9.0000000000001446

    Figure Lengend Snippet: PAR2 activation in the colon by 2F sensitizes LSN responses to mechanical and chemical stimulation of the colon via endosomal internalization, PKA and PKC. LSN action potential firing after mechanical distention: gradually increasing intraluminal pressure from 0 to 80 mm Hg, and chemical application: 1 mM cinnamaldehyde and 1 µM capsaicin are illustrated in (A). (B) Responses to distention were quantified through the change in firing rate over increasing discrete pressure values. (C and D) Responses to chemical stimuli were quantified through the change in firing rate over time after application (vertical dotted line). 100 µM 2F stimulation, but not vehicle control, elicited sensitization of the LSN to (B) distention, (C) cinnamaldehyde, and (D) capsaicin. Pretreatment with PS2 before 2F stimulation reduced subsequent LSN responses to distention, cinnamaldehyde, and capsaicin compared with PN2. Pretreatment with H-89 and GFX, applied either simultaneously or individually, also reduced subsequent peak LSN responses to distention, cinnamaldehyde, and capsaicin compared with DMSO vehicle. (B–D) Independent samples t tests and one-way ANOVA with post-hoc FDR-corrected independent samples t test (N = 5–9). * P < 0.05, ** P < 0.01, **** P < 0.0001. Data are presented as means ± SD. LSN, lumbar splanchnic nerve; PAR2, protease-activated receptor 2; PKA, protein kinase A; PKC, protein kinase C.

    Article Snippet: When measuring the inhibitory concentration 50 (IC50), cells received PAR650097 mIgG or hIgG or isotype control antibodies, for 60 minutes at room temperature before addition of PAR2 activating matriptase (3946-SEB-010, R&D Systems, Abingdon, United Kingdom).

    Techniques: Activation Assay, Control

    Structures of the masking reagent consist of: 1) a maleimide for conjugation, 2) a matriptase‐cleavable peptide (highlighted in purple), and 3) PEG to create steric hindrance, preventing the antibody from binding to the target.

    Journal: Chembiochem

    Article Title: Design and Characterization of Prodrugged Anti‐CTLA‐4 Antibodies

    doi: 10.1002/cbic.202500304

    Figure Lengend Snippet: Structures of the masking reagent consist of: 1) a maleimide for conjugation, 2) a matriptase‐cleavable peptide (highlighted in purple), and 3) PEG to create steric hindrance, preventing the antibody from binding to the target.

    Article Snippet: Prodrugged antibody was incubated with human matriptase (30:1 molar ratio, R&D system, 3946‐SE‐010) in 100 mM Tris buffer, pH7.6 at 37C.

    Techniques: Conjugation Assay, Binding Assay