Journal: The Journal of clinical endocrinology and metabolism

Article Title: β2-Agonist Induces Net Leg Glucose Uptake and Free Fatty Acid Release at Rest but Not During Exercise in Young Men.

doi: 10.1210/jc.2018-01349

Figure Lengend Snippet: Figure 5. Muscle protein phosphorylation and representative Western blots for terbutaline (filled bars) and placebo (open bars) at rest and at cessation of knee extensor exercise. (a) Phosphorylation of PKA substrateSer/Thr. (b) Phosphorylation ratio of p-AktSer473/Akt total. (c) Representative blots. Values are means and upper bound of the 95% CI (n = 9 for rest; n = 8 for exercise). *P , 0.05, for treatment difference at sampling point. EX, cessation of exercise; R, rest.

Article Snippet: Primary antibodies used were p-PKA substrateSer/Thr (dilution, 1:500; no. 9621, Cell Signaling Technology, Leiden, Netherlands), Akt (dilution, 1:1000; no. 2967, Cell Signaling Technology), and p-AktSer473 (dilution, 1:1000; no. 9271, Cell Figure 1.

Techniques: Phospho-proteomics, Western Blot, Sampling

Journal: Oncotarget

Article Title: The serine protease matriptase inhibits migration and proliferation in multiple myeloma cells

doi: 10.18632/oncotarget.28300

Figure Lengend Snippet: ( A – C ) Nanostring mRNA expression screening of (A) matriptase (ST14), (B) HAI-1 (SPINT1) and (C) HAI-2 (SPINT2) was performed on human myeloma cell lines ( n = 8). ( D , E ) Matriptase (D) overexpression and (E) knockdown was confirmed via Western blotting.

Article Snippet: A plasmid containing a shRNA specific for matriptase (sc-43911-V) and control plasmid (sc-108080) purchased from Santa Cruz Biotechnology was used to establish cells with stable knocked-down matriptase (RPMI-8226 shMatriptase and JJN-3 shMatriptase) and control cell lines (RPMI-8226 shMock and JJN-3 shMock), respectively.

Techniques: Expressing, Over Expression, Knockdown, Western Blot

Journal: Oncotarget

Article Title: The serine protease matriptase inhibits migration and proliferation in multiple myeloma cells

doi: 10.18632/oncotarget.28300

Figure Lengend Snippet: Protein expression of HAI-1 and HAI-2 was determined using Western blotting. ( A ) INA-6 and U266 matriptase overexpression (Matriptase) and control (Mock) cells, and ( B ) RPMI-8226 and JJN-3 matriptase knockdown (shMatriptase) and control (shMock) cells. One representative of at least three independent experiments is shown.

Article Snippet: A plasmid containing a shRNA specific for matriptase (sc-43911-V) and control plasmid (sc-108080) purchased from Santa Cruz Biotechnology was used to establish cells with stable knocked-down matriptase (RPMI-8226 shMatriptase and JJN-3 shMatriptase) and control cell lines (RPMI-8226 shMock and JJN-3 shMock), respectively.

Techniques: Expressing, Western Blot, Over Expression, Control, Knockdown

Journal: Oncotarget

Article Title: The serine protease matriptase inhibits migration and proliferation in multiple myeloma cells

doi: 10.18632/oncotarget.28300

Figure Lengend Snippet: Cell proliferation was measured in INA-6 Matriptase overexpression (Matriptase) and control (Mock) cells by the CellTiter-Glo assay. The mean (±SD) of four independent experiments is shown. Each dot represents one technical replicate and dots in the same color correspond to one of the biological replicates. Triangles represent the mean of each biological replicate. p -value was calculated by unpaired Student’s t -test based on the average from each independent experiment. * p ≤ 0.05.

Article Snippet: A plasmid containing a shRNA specific for matriptase (sc-43911-V) and control plasmid (sc-108080) purchased from Santa Cruz Biotechnology was used to establish cells with stable knocked-down matriptase (RPMI-8226 shMatriptase and JJN-3 shMatriptase) and control cell lines (RPMI-8226 shMock and JJN-3 shMock), respectively.

Techniques: Over Expression, Control, Glo Assay

Journal: Oncotarget

Article Title: The serine protease matriptase inhibits migration and proliferation in multiple myeloma cells

doi: 10.18632/oncotarget.28300

Figure Lengend Snippet: ( A , B ) Migration of (A) INA-6 and (B) U266 matriptase overexpression (Matriptase) and control (Mock) cells. The promigratory cytokines HGF and/or SDF-1α were added to the lower wells as indicated. ( C ) Basal migration of matriptase knockdown (shMatriptase) and control cells (shMock) in RPMI-8226 and JJN-3. ( D ) Src activation in INA-6 Matriptase and Mock. Cells were starved for 6 h in serum-free medium. Subsequently, cells were stimulated with HGF and SDF-1α for 10 and 30 min and probed with antibodies as indicated. ( E ) Migration of INA-6 Matriptase and Mock cells. The combination of HGF and SDF-1α were added to the lower wells in all conditions. DMSO or PP2 Src inhibitor were added in concentrations as indicated to both upper and lower wells. (A–C and E) Cells were seeded in the upper well of a two-chamber transwell migration assay. After 24 h incubation, cells in the bottom wells were counted and the percentage of migrated cells calculated. Bars in (A), (B), (C) and (E) represent the mean (±SD) of at least two repeated counts in two independent measurements. Concentrations of HGF and SDF-1α was 150 ng/mL and 75 ng/mL, respectively, in all experiments. One representative of three independent experiments is shown in all figures. p -values were calculated by unpaired Student’s t -test. ns = not significant ( p > 0.05), * p ≤ 0.05, *** p ≤ 0.001, **** p ≤ 0.0001.

Article Snippet: A plasmid containing a shRNA specific for matriptase (sc-43911-V) and control plasmid (sc-108080) purchased from Santa Cruz Biotechnology was used to establish cells with stable knocked-down matriptase (RPMI-8226 shMatriptase and JJN-3 shMatriptase) and control cell lines (RPMI-8226 shMock and JJN-3 shMock), respectively.

Techniques: Migration, Over Expression, Control, Knockdown, Activation Assay, Transwell Migration Assay, Incubation