leukadherin 1 la1  (MedChemExpress)

Journal: Journal of Inflammation Research

Article Title: Microglial Activation Mediates Noradrenergic Locus Coeruleus Neurodegeneration via Complement Receptor 3 in a Rotenone-Induced Parkinson’s Disease Mouse Model

doi: 10.2147/JIR.S299927

Figure Lengend Snippet: LA-1 abrogates rotenone-induced activation and M1 polarization of microglia in mice. ( A ) Immunohistochemistry with an anti-Iba-1 antibody was performed to stain microglial cells in the LC of rotenone-intoxicated mice with or without LA-1 treatment, and representative images are shown. ( B ) Quantification of the density of Iba-1 immunostaining. Results were mean ± SEM from five mice for each group and were analyzed by one-way ANOVA (F (3,16) = 10.109, P = 0.001). ( C ) The mRNA levels of iNOS, TNFα and IL-1β in the brainstem of rotenone-treated mice with or without LA-1 treatment were determined by real-time PCR. Results were mean ± SEM from five mice for each group and were analyzed by one-way ANOVA (iNOS: F (3,16) = 5.661, P = 0.008; TNFα: F (3,16) = 7.439, P = 0.002; IL-1β: F (3,16) = 3.589, P = 0.037; post hoc analysis by Tukey’s multiple comparisons test). ( D ) The mRNA levels of Arg-1, CD206 and YM-1 in the brainstem of rotenone-treated mice with or without LA-1 treatment were determined by real-time PCR. Results were mean ± SEM from five mice for each group and were analyzed by one-way ANOVA (Arg-1: F (3,16) = 12.575, P = 0.000, post hoc analysis by Tukey’s multiple comparisons test; CD206: F (3,16) = 6.956, P=0.003, post hoc analysis by Tamhane’s T2 multiple comparisons test; YM-1: F (3,16) = 10.109, P=0.001, post hoc analysis by Tukey’s multiple comparisons test). *P<0.05, **P<0.01; Scale bar = 100 μm.

Article Snippet: Mice received (i.p.) 500 μL of 50 μM LA-1 (S8306, Selleck, China) or vehicle 1 h after rotenone injection for 3 continuous weeks.

Techniques: Activation Assay, Immunohistochemistry, Staining, Immunostaining, Real-time Polymerase Chain Reaction

Journal: Journal of Inflammation Research

Article Title: Microglial Activation Mediates Noradrenergic Locus Coeruleus Neurodegeneration via Complement Receptor 3 in a Rotenone-Induced Parkinson’s Disease Mouse Model

doi: 10.2147/JIR.S299927

Figure Lengend Snippet: Genetic deletion of CR3 or LA-1 treatment mitigates oxidative stress in rotenone-intoxicated mice. ( A ) The MDA and GSH contents were determined in the brainstem of WT and CR3 −/- mice after rotenone treatment by using commercial kits. Results were mean ± SEM from three mice for each group and were analyzed by two-way ANOVA (MDA: F (3,8) = 34.604, P = 0.000; GSH: F (3,8) = 37.129, P = 0.000; post hoc analysis by Tukey’s multiple comparisons test). ( B ) The MDA and GSH contents were determined in the brainstem of rotenone-treated mice with or without LA-1 treatment by using commercial kits. Results were mean ± SEM from four mice for each group and were analyzed by one-way ANOVA (MDA: F (3,12) = 12.753, P = 0.000; GSH: F (3,12) = 10.373, P = 0.001; post hoc analysis by Tukey’s multiple comparisons test). *P<0.05, **P<0.01.

Article Snippet: Mice received (i.p.) 500 μL of 50 μM LA-1 (S8306, Selleck, China) or vehicle 1 h after rotenone injection for 3 continuous weeks.

Techniques:

Journal: Journal of Inflammation Research

Article Title: Microglial Activation Mediates Noradrenergic Locus Coeruleus Neurodegeneration via Complement Receptor 3 in a Rotenone-Induced Parkinson’s Disease Mouse Model

doi: 10.2147/JIR.S299927

Figure Lengend Snippet: CR3 deficiency or LA-1 treatment alleviates rotenone-induced degeneration of LC/NE neurons in mice. ( A ) Immunohistochemistry with an anti-TH antibody was performed to stain LC/NE neurons of rotenone-intoxicated WT and CR3 −/- mice, and representative images are shown. ( B ) Quantification of the number of THir neurons. Results were mean ± SEM from five to six mice for each group and were analyzed by two-way ANOVA (F (3,19) = 6.923, P = 0.002, post hoc analysis by Tukey’s multiple comparisons test). ( C ) Immunohistochemistry with an anti-TH antibody was performed to stain LC/NE neurons of rotenone-intoxicated mice with or without LA-1 treatment, and representative images are shown. ( D ) Quantification of the number of THir neurons. Results were mean ± SEM from five mice for each group and were analyzed by one-way ANOVA (F (3,16) = 27.527, P = 0.000, post hoc analysis by Tukey’s multiple comparisons test). **P<0.01; Scale bar = 100 μm.

Article Snippet: Mice received (i.p.) 500 μL of 50 μM LA-1 (S8306, Selleck, China) or vehicle 1 h after rotenone injection for 3 continuous weeks.

Techniques: Immunohistochemistry, Staining

Journal: bioRxiv

Article Title: Pharmacologic manipulation of complement receptor 3 prevents dendritic spine loss and cognitive impairment after acute cranial radiation

doi: 10.1101/2020.11.25.398701

Figure Lengend Snippet: a) Experimental paradigm identical to the previous experiment with the exception of daily LA1 injections for 30 d. b )Modified legend with new symbols for this cohort of mice. NOR ( c ) and LIII ( d ) demonstrated a significant irradiation-induced deficit in vehicle task performance that was prevented in LA1 treated mice while e ) FC did not demonstrate a difference in between groups. Quantification of confocal images displaying f ) Thy1-YFP spine density, g ) CD68, and h ) CD11b. n = 10 ( c-e ) and n = 5 ( f-h ) per group; c, e-h ) two-way ANOVA followed by multiple comparisons correction; d ) Mantel-Cox test and test for trend; * p < 0.05, *** p < 0.001, **** p < 0.0001.

Article Snippet: Animal cohort 2, consisting of 40 Thy1 YFP/YFP :: CR3 +/+ male mice, were randomly assigned to either LA1 (Leukadherin-1; S8306, Selleckchem, TX) or) or vehicle (6% DMSO (Dimethyl Sulfoxide, ACS; 191418, MP Biomedicals, PA) in sterile saline with 1% Tween-20 (BP337500; Fisher Scientific, NJ)).

Techniques: Modification, Irradiation

Journal: Frontiers in Immunology

Article Title: Identification of a Novel HIF-1α-α M β 2 Integrin-NET Axis in Fibrotic Interstitial Lung Disease

doi: 10.3389/fimmu.2020.02190

Figure Lengend Snippet: Specific α M β 2 activation induces NET-like structure release. The effects of specific α M β 2 activation upon on neutrophil function by culturing cells with varying concentrations of leukadherin-1 (LA-1). (A) Lower concentrations (0–15 μM) of LA-1 did not increased neutrophil adhesion compared to vehicle. From 20 to 50 μM LA-1, we observed a dose dependent increase in neutrophil adhesion. Data are presented as the mean ± SEM from three independent experiments and analyzed by two-way ANOVA with a Sidak's multiple comparison test. The release of NET-like structures was visualized by staining nuclear DNA (DAPI, blue staining) and histone H3 (green staining) in neutrophils. (B) Untreated neutrophils and (C) neutrophils stimulated with 10 μM LA-1 displayed punctate nuclear staining. (D) 25 μM LA-1 stimulation induced greater neutrophil adhesion, however nuclear staining was still punctate. (E) Greater numbers of neutrophils were seen following stimulation with 100 μM LA-1 with the addition of externalized DNA and histone staining, forming NET-like structures. (F) 200 μM LA-1 stimulation also induced NET releasing neutrophils. Representative images are shown from two independent experiments. *** = p = 0.0003, **** = p < 0.0001.

Article Snippet: In brief, 5 ×10 5 neutrophils were added to fibrinogen-coated coverslips, stimulated for 4 h with 40 nM PMA, 0.5 mM MnCl 2 or varying concentrations of leukadherin-1 (LA-1; Sigma, UK), and fixed with 4% PFA.

Techniques: Activation Assay, Staining