Src inhibition attenuates polyglutamine-mediated neuromuscular degeneration in spinal and bulbar muscular atrophy Nature Communications, 2019 Sep 19

"Downstream targets of Src in mouse and cellular models of SBMA. a – c Immunoblots showing the levels of phosphorylated and total <t>p130Cas,</t> Stat3, p38MAPK, and Akt proteins in the spinal cord and skeletal muscle of 9-week-old AR-97Q mice treated with or without A419259 ( a ). Quantitative analyses were performed using densitometry ( b , c ). d – f Immunoblots showing the levels of the phosphorylated and total Src, p130Cas, Stat3, p38MAPK, and Akt proteins in DHT-treated NSC34 and C2C12 cells stably expressing AR-97Q with or without 0.2 µM of A419259 administration ( d ). Quantitative analyses were performed using densitometry ( e , f ). g – i Immunoblots showing the levels of the phosphorylated and total Src, p130Cas, Stat3, and p38MAPK proteins in the NSC34 and C2C12 cells transfected with mock or Src plasmids ( g ). Quantitative analyses were performed using densitometry ( h , i ). Quantitative analyses were performed on samples from n = 3 animals per group. Error bars indicate the s.e.m. Statistical analyses were performed using the unpaired two-sided t -test ( b , c , e , f , h , i ). * p < 0.05 and ** p < 0.01. N.S., not significant. Source data are provided as a Source Data file ( a – i ) "