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phospho interferon regulatory factor 3 p irf3 antibody  (Proteintech)


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    Structured Review

    Proteintech phospho interferon regulatory factor 3 p irf3 antibody
    ( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis <t>of</t> <t>p-IRF3</t> expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
    Phospho Interferon Regulatory Factor 3 P Irf3 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 507 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/irf3/IRF3+Antibody/pmc13674266-59-6-15
    Average 96 stars, based on 507 article reviews
    phospho interferon regulatory factor 3 p irf3 antibody - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "A Multifunctional Metal-Based Nanomedicine for Copper Sequestration, in situ Toxification, and Immune Activation Against Metastatic Cancer"

    Article Title: A Multifunctional Metal-Based Nanomedicine for Copper Sequestration, in situ Toxification, and Immune Activation Against Metastatic Cancer

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S580749

    ( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis of p-IRF3 expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
    Figure Legend Snippet: ( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis of p-IRF3 expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

    Techniques Used: Immunofluorescence, Expressing, Membrane, Fluorescence, Analysis, Western Blot

    Related Articles

    other:

    Article Title: A dual role for ER-Golgi cargo receptor LMAN1 in supporting CSFV replication and restraining RLR signaling.
    Article Snippet: Antibodies against Flag (20543-1-AP), LMAN1 (20543-1-AP), RIG-1/DDX58 (20566-1-AP), IFIH1/MDA5 (21775-1-AP), MAVS (14341-1-AP), IRF3 (11312-1-AP), NF-κB p65 (66535-1-Ig), p-NF-κB p65 (Ser468) (82335-1-RR), and CANX (10427-2-AP / 66903-1-Ig) were obtained from Proteintech.

    Article Title: PIM1 Inhibition Alleviates Aspergillus fumigatus Keratitis by Regulating DDX41-Mediated STING Signaling Pathway.
    Article Snippet: Antibodies targeting PIM2 (25865-1-AP, 1:1000), PIM3 (33202-1-AP, 1:1000), β-actin (20536-1-AP, 1:50,000), GST (10000-0-AP, 1:2000), MYC (16286-1-AP, 1:2000), Flag (66008-4-Ig, 1:2000), His (66005-1-Ig, 1:2000), STING (19851-1-AP, 1:2000), IRF3 (11312-1-AP, 1:1000), and TBK1 (28397-1-AP, 1:1000) were sourced from Proteintech (Wuhan, China).

    Article Title: PIM1 Inhibition Alleviates Aspergillus fumigatus Keratitis by Regulating DDX41-Mediated STING Signaling Pathway
    Article Snippet: Antibodies targeting PIM2 (25865-1-AP, 1:1000), PIM3 (33202-1-AP, 1:1000), β-actin (20536-1-AP, 1:50,000), GST (10000-0-AP, 1:2000), MYC (16286-1-AP, 1:2000), Flag (66008-4-Ig, 1:2000), His (66005-1-Ig, 1:2000), STING (19851-1-AP, 1:2000), IRF3 (11312-1-AP, 1:1000), and TBK1 (28397-1-AP, 1:1000) were sourced from Proteintech (Wuhan, China).

    Hemagglutination Assay:

    Article Title: The segmented flavivirus ALSV-encoded nucleoprotein VP2 inhibits type I interferon production by targeting RIG-I
    Article Snippet: To induce an IFN-I response, HEK293T cells were transfected with poly(I:C) (Invivogen, Cat# tlrl-pic) for the specified durations. .. The following primary antibodies were utilized: glyceraldehyde 3-phosphate dehydrogenase (GAPDH; ProteinTech, cat#10494-1-AP), HA (ProteinTech, cat#51064-2-AP), GST (ProteinTech, cat#10000-0-AP), Flag (ProteinTech, cat#20543-1-AP), Myc (ProteinTech, cat#60003-2-Ig), β-Actin (ProteinTech, cat#66009-1-Ig), IFIT3 (ProteinTech, cat#15201-1-AP), IFIT1 (Cell Signaling Technology, cat#14769), Phospho-IRF3 (Abways, cat#CY6575), Phospho-TBK1 (Cell Signaling Technology, cat#5483s), IRF3 (ProteinTech, cat11312-1-AP), TBK1 (Abcam, cat#AB40676), RIG-I (ProteinTech, cat#20566-1-AP), MDA5 (ProteinTech, cat#21775-1-AP), MAVS (ProteinTech, cat#14341-1-AP), TRAF3 (ProteinTech, cat#18099-1-AP), LC3 (ProteinTech, cat#14600-1-AP), and ATG5 (HUABIO, cat#ET1611-38). ..

    Cell-Signaling:

    Article Title: The segmented flavivirus ALSV-encoded nucleoprotein VP2 inhibits type I interferon production by targeting RIG-I
    Article Snippet: To induce an IFN-I response, HEK293T cells were transfected with poly(I:C) (Invivogen, Cat# tlrl-pic) for the specified durations. .. The following primary antibodies were utilized: glyceraldehyde 3-phosphate dehydrogenase (GAPDH; ProteinTech, cat#10494-1-AP), HA (ProteinTech, cat#51064-2-AP), GST (ProteinTech, cat#10000-0-AP), Flag (ProteinTech, cat#20543-1-AP), Myc (ProteinTech, cat#60003-2-Ig), β-Actin (ProteinTech, cat#66009-1-Ig), IFIT3 (ProteinTech, cat#15201-1-AP), IFIT1 (Cell Signaling Technology, cat#14769), Phospho-IRF3 (Abways, cat#CY6575), Phospho-TBK1 (Cell Signaling Technology, cat#5483s), IRF3 (ProteinTech, cat11312-1-AP), TBK1 (Abcam, cat#AB40676), RIG-I (ProteinTech, cat#20566-1-AP), MDA5 (ProteinTech, cat#21775-1-AP), MAVS (ProteinTech, cat#14341-1-AP), TRAF3 (ProteinTech, cat#18099-1-AP), LC3 (ProteinTech, cat#14600-1-AP), and ATG5 (HUABIO, cat#ET1611-38). ..

    Article Title: Molecularly cyclic dinucleotide-based immune stimulator-aptamer conjugate enhances systemic STING-driven antitumor immunity
    Article Snippet: Activation of the stimulator of interferon genes (STING) pathway has emerged as a promising approach in cancer immunotherapy.. Although cyclic dinucleotide (CDN)-based STING agonists have demonstrated considerable antitumor potential in preclinical studies, their clinical translation has been hampered by multiple limitations, including poor metabolic stability, low cellular uptake, limited tumor accumulation, and off-target toxicity.. To address these challenges, we developed a tumor-targeting immune stimulator-aptamer conjugate (ISApC) by conjugating the phosphorothioate isomer of CDN (F2) to an aptamer Sgc8c via a cleavable linker (F2-L1-Sgc8c).



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    ( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis <t>of</t> <t>p-IRF3</t> expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
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    ( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis <t>of</t> <t>p-IRF3</t> expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
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    ( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis <t>of</t> <t>p-IRF3</t> expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
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    Image Search Results


    ( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis of p-IRF3 expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

    Journal: International Journal of Nanomedicine

    Article Title: A Multifunctional Metal-Based Nanomedicine for Copper Sequestration, in situ Toxification, and Immune Activation Against Metastatic Cancer

    doi: 10.2147/IJN.S580749

    Figure Lengend Snippet: ( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis of p-IRF3 expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

    Article Snippet: The calreticulin (CRT), CD206, CD86, and phospho-interferon regulatory factor 3 (p-IRF3) antibody were purchased from Proteintech Group, Inc. (Wuhan, China).

    Techniques: Immunofluorescence, Expressing, Membrane, Fluorescence, Analysis, Western Blot