Journal: International Journal of Molecular Sciences

Article Title: Arginine Is a Novel Drug Target for Arginine Decarboxylase in Human Colorectal Cancer Cells

doi: 10.3390/ijms241813741

Figure Lengend Snippet: ADC performed better than ADI in ASS-positive human colorectal cancer cell lines and was less toxic to normal cells. ( A ) Immunoblot analysis showing the expression of ASS in colorectal cancer cells. ( B ) Cell viability towards ADI of different cancer cell lines. ( C ) Cell viability towards ADC of different cancer cell lines and normal human fibroblast HFF1. ( D ) Bar chart comparing the effects of AFM and 100 μg/mL ADC on the viability of HCT116 and COLO 205 cells. Cells were treated for 72 h before MTT analysis. ( E ) Cell viability towards ADC in rat primary hepatocytes. Data are expressed as the percentage of viable cells compared to control (complete medium) in the form of mean ± SEM of three individual experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 using Mann–Whitney U test (versus control). # p < 0.05, ## p < 0.01, ### p < 0.001 using one-way ANOVA followed by Bonferroni test (between groups).

Article Snippet: Human foreskin fibroblast cell line HFF1 was purchased from the American Type Culture Collection (Manassas, VA, U.S.).

Techniques: Western Blot, Expressing, MANN-WHITNEY

Journal: PLOS Pathogens

Article Title: Function and regulation of a steroidogenic CYP450 enzyme in the mitochondrion of Toxoplasma gondii

doi: 10.1371/journal.ppat.1011566

Figure Lengend Snippet: (A) Plaque assays. Analysis of parasite growth for 7 days after fibroblast infection with 150 parasites from the parental and ΔTgMAPR strains showing representative images and quantification of lysed area from 3 independent experiments. Data are means ± SD. (B) Gliding assays. Freshly lysed ΔTgMAPR1 and parental parasites were allowed to glide on FBS-coated glass slides for 30 min before fixation and staining with anti-SAG1 antibody under non-permeabilized conditions. Representative images are shown. Measurement of gliding trails by dot plots, showing means ± SD of 3 independent experiments. (C) Invasion assays. Quantification of invasion of parental and ΔTgMAPR parasites using the red/green invasion assay. Red histograms represent external, attached parasites while green histograms represent internal, penetrated parasites. Data are means ± SEM of 4 independent experiments. (D) [ 3 H]uracil incorporation assays for replication. HFF were infected with parental and ΔTgMAPR parasites for 24 h prior to incubation with tritiated uracil. Data are means ± SD of 3 independent experiments. (E) Induced egress assays. HFF were infected with parental and ΔTgMAPR parasites for 24 h prior to exposure to 2 μM A23187 for time-lapse microscopy views, showing rapid egress for parental parasites that were largely extracellular upon treatment and slower response for the treated mutant (arrows). Quantification of egress time with means in seconds for 3 treated monolayers infected with parental or ΔTgMAPR parasites.

Article Snippet: Human foreskin fibroblasts (HFF), VERO cells and HeLa cells obtained from the American Type Culture Collection (Manassas, VA), and immortalized HFF (hTERT) received from S.N.J.

Techniques: Infection, Staining, Invasion Assay, Incubation, Time-lapse Microscopy, Mutagenesis