p hsf1 ser326  (Bioss)

Journal: Scientific Reports

Article Title: HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2—Dectin-1/ SYK mediated ROS and inflammatory responses

doi: 10.1038/s41598-025-13531-0

Figure Lengend Snippet: Bioinformatics analysis. A: Box plot for sample correction. B: Density plot of sample expression. C: Sample quantile plot. D: Volcano plot of differentially expressed genes (DEGs). E: Mean difference plot of DEGs. F: Bar chart of GO enrichment analysis. G: Chord diagram of KEGG enrichment analysis. H: Protein-Protein Interaction (PPI) network diagram. I: Scatter plot of ROS and SIRT1 expression levels. J: Scatter plot of ROS and P38 expression levels. K: Scatter plot of SIRT1 and HSF1 expression levels.

Article Snippet: Macrophage RAW264.7 cells were obtained from the Chinese Cell Resource Center; fetal bovine serum and DMEM medium were purchased from Gibco; ovalbumin (OVA) was obtained from Sigma (USA); LPS was purchased from Sigma; GSH, MDA, IL-1β, and TNFα ELISA kits were purchased from Wuhan Elabscience Biotechnology Co., Ltd.; HSF1 activator HSF1A was obtained from Beijing Biolabs Technology Co., Ltd.; HSF1 inhibitor KRIBB11 was purchased from MCE Company; SIRPα-OE vector and SIRPα shRNA were obtained from Genscript (Nanjing, China); INTERFERin ® kit was purchased from Selleck Company; Masson staining kit was purchased from Beijing Biolabs Technology Co., Ltd.; Western blot electrophoresis equipment and the FlexiVent pulmonary function testing system were purchased from Shanghai Sitek Biological Technology Co., Ltd. Fluorescent primary antibody: recombinant Anti-CD68 antibody (ab53444, Abcam).

Techniques: Expressing

Journal: Scientific Reports

Article Title: HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2—Dectin-1/ SYK mediated ROS and inflammatory responses

doi: 10.1038/s41598-025-13531-0

Figure Lengend Snippet: HSF1 improves lung function and lung injury in asthmatic mice. A: Masson staining of lung tissue changes in the Sham, Model, and Model + HSF1A groups. Scale: 100 μm. B: Immunofluorescence showing CD68 levels in the Sham, Model, and Model + HSF1A groups. Scale: 50 μm. C: Assessment of lung function in mice from the Sham, Model, and Model + HSF1A groups, including FEV1, FVC, FEV1/FVC, FEF 25%, and Ri changes. Data are expressed as mean ± standard deviation. N = 7; Sham vs. Model: * P < 0.05; ** P < 0.01; Model vs. Model + HSF1A: # P < 0.05; ## P < 0.01.

Article Snippet: Macrophage RAW264.7 cells were obtained from the Chinese Cell Resource Center; fetal bovine serum and DMEM medium were purchased from Gibco; ovalbumin (OVA) was obtained from Sigma (USA); LPS was purchased from Sigma; GSH, MDA, IL-1β, and TNFα ELISA kits were purchased from Wuhan Elabscience Biotechnology Co., Ltd.; HSF1 activator HSF1A was obtained from Beijing Biolabs Technology Co., Ltd.; HSF1 inhibitor KRIBB11 was purchased from MCE Company; SIRPα-OE vector and SIRPα shRNA were obtained from Genscript (Nanjing, China); INTERFERin ® kit was purchased from Selleck Company; Masson staining kit was purchased from Beijing Biolabs Technology Co., Ltd.; Western blot electrophoresis equipment and the FlexiVent pulmonary function testing system were purchased from Shanghai Sitek Biological Technology Co., Ltd. Fluorescent primary antibody: recombinant Anti-CD68 antibody (ab53444, Abcam).

Techniques: Staining, Immunofluorescence, Standard Deviation

Journal: Scientific Reports

Article Title: HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2—Dectin-1/ SYK mediated ROS and inflammatory responses

doi: 10.1038/s41598-025-13531-0

Figure Lengend Snippet: HSF1 promotes SIRPα activity and inhibits p-SYK/t-SYK and Dectin-1 function. A: ELISA measuring levels of GSH, MDA, IL-1β, and TNFα in the Sham, Model, and Model + HSF1A groups. B: Western blot analysis of protein expression for P47 (ROS), p-P38/t-P38, p-SIRT1/t-SIRT1, HSF1, SIRPα, p-SYK/t-SYK, and Dectin-1 in the Sham, Model, and Model + HSF1A groups. GAPDH as control protein. Data are expressed as mean ± standard deviation. N = 7; Sham vs. Model: * P < 0.05; ** P < 0.01; Model vs. Model + HSF1A: # P < 0.05; ## P < 0.01.

Article Snippet: Macrophage RAW264.7 cells were obtained from the Chinese Cell Resource Center; fetal bovine serum and DMEM medium were purchased from Gibco; ovalbumin (OVA) was obtained from Sigma (USA); LPS was purchased from Sigma; GSH, MDA, IL-1β, and TNFα ELISA kits were purchased from Wuhan Elabscience Biotechnology Co., Ltd.; HSF1 activator HSF1A was obtained from Beijing Biolabs Technology Co., Ltd.; HSF1 inhibitor KRIBB11 was purchased from MCE Company; SIRPα-OE vector and SIRPα shRNA were obtained from Genscript (Nanjing, China); INTERFERin ® kit was purchased from Selleck Company; Masson staining kit was purchased from Beijing Biolabs Technology Co., Ltd.; Western blot electrophoresis equipment and the FlexiVent pulmonary function testing system were purchased from Shanghai Sitek Biological Technology Co., Ltd. Fluorescent primary antibody: recombinant Anti-CD68 antibody (ab53444, Abcam).

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control, Standard Deviation

Journal: Scientific Reports

Article Title: HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2—Dectin-1/ SYK mediated ROS and inflammatory responses

doi: 10.1038/s41598-025-13531-0

Figure Lengend Snippet: In macrophages, HSF1 mediates ROS and inflammatory responses by regulating the SIRPα/Dectin-1/SYK balance, thereby inhibiting asthma progression. A: Western blot analysis of HSF1, SIRPα, p-SYK/t-SYK, Dectin-1, P22, P47, and p-P38/t-P38 protein expression in the NC, OVA + LPS, HSF1A + OVA + LPS, KRIBB11 + OVA + LPS, and SIRPα-OE + KRIBB11 + OVA + LPS groups. B: ELISA measurements of GSH, MDA, IL-1β, and TNFα levels in the NC, OVA + LPS, HSF1A + OVA + LPS, KRIBB11 + OVA + LPS, and SIRPα-OE + KRIBB11 + OVA + LPS groups. GAPDH as control protein. Data are expressed as mean ± standard deviation. N = 3; NC vs. OVA + LPS: ** P < 0.01; ns P >0.05; OVA + LPS vs. HSF1A + OVA + LPS: # P < 0.05; ## P < 0.01; OVA + LPS vs. KRIBB11 + OVA + LPS: & P < 0.05; && P < 0.01; ns P >0.05; KRIBB11 + OVA + LPS vs. SIRPα + KRIBB11 + OVA + LPS: †† P < 0.01; ns P >0.05.

Article Snippet: Macrophage RAW264.7 cells were obtained from the Chinese Cell Resource Center; fetal bovine serum and DMEM medium were purchased from Gibco; ovalbumin (OVA) was obtained from Sigma (USA); LPS was purchased from Sigma; GSH, MDA, IL-1β, and TNFα ELISA kits were purchased from Wuhan Elabscience Biotechnology Co., Ltd.; HSF1 activator HSF1A was obtained from Beijing Biolabs Technology Co., Ltd.; HSF1 inhibitor KRIBB11 was purchased from MCE Company; SIRPα-OE vector and SIRPα shRNA were obtained from Genscript (Nanjing, China); INTERFERin ® kit was purchased from Selleck Company; Masson staining kit was purchased from Beijing Biolabs Technology Co., Ltd.; Western blot electrophoresis equipment and the FlexiVent pulmonary function testing system were purchased from Shanghai Sitek Biological Technology Co., Ltd. Fluorescent primary antibody: recombinant Anti-CD68 antibody (ab53444, Abcam).

Techniques: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Control, Standard Deviation

Journal: Scientific Reports

Article Title: HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2—Dectin-1/ SYK mediated ROS and inflammatory responses

doi: 10.1038/s41598-025-13531-0

Figure Lengend Snippet: Mechanistic study of how HSF1 inhibits asthma progression through the SIRPα/Dectin-1/SYK balance-mediated ROS and inflammatory responses in macrophages (Created with BioRender.com).

Article Snippet: Macrophage RAW264.7 cells were obtained from the Chinese Cell Resource Center; fetal bovine serum and DMEM medium were purchased from Gibco; ovalbumin (OVA) was obtained from Sigma (USA); LPS was purchased from Sigma; GSH, MDA, IL-1β, and TNFα ELISA kits were purchased from Wuhan Elabscience Biotechnology Co., Ltd.; HSF1 activator HSF1A was obtained from Beijing Biolabs Technology Co., Ltd.; HSF1 inhibitor KRIBB11 was purchased from MCE Company; SIRPα-OE vector and SIRPα shRNA were obtained from Genscript (Nanjing, China); INTERFERin ® kit was purchased from Selleck Company; Masson staining kit was purchased from Beijing Biolabs Technology Co., Ltd.; Western blot electrophoresis equipment and the FlexiVent pulmonary function testing system were purchased from Shanghai Sitek Biological Technology Co., Ltd. Fluorescent primary antibody: recombinant Anti-CD68 antibody (ab53444, Abcam).

Techniques:

Journal: Scientific Reports

Article Title: HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2—Dectin-1/ SYK mediated ROS and inflammatory responses

doi: 10.1038/s41598-025-13531-0

Figure Lengend Snippet: Bioinformatics analysis. A: Box plot for sample correction. B: Density plot of sample expression. C: Sample quantile plot. D: Volcano plot of differentially expressed genes (DEGs). E: Mean difference plot of DEGs. F: Bar chart of GO enrichment analysis. G: Chord diagram of KEGG enrichment analysis. H: Protein-Protein Interaction (PPI) network diagram. I: Scatter plot of ROS and SIRT1 expression levels. J: Scatter plot of ROS and P38 expression levels. K: Scatter plot of SIRT1 and HSF1 expression levels.

Article Snippet: Macrophage RAW264.7 cells were obtained from the Chinese Cell Resource Center; fetal bovine serum and DMEM medium were purchased from Gibco; ovalbumin (OVA) was obtained from Sigma (USA); LPS was purchased from Sigma; GSH, MDA, IL-1β, and TNFα ELISA kits were purchased from Wuhan Elabscience Biotechnology Co., Ltd.; HSF1 activator HSF1A was obtained from Beijing Biolabs Technology Co., Ltd.; HSF1 inhibitor KRIBB11 was purchased from MCE Company; SIRPα-OE vector and SIRPα shRNA were obtained from Genscript (Nanjing, China); INTERFERin ® kit was purchased from Selleck Company; Masson staining kit was purchased from Beijing Biolabs Technology Co., Ltd.; Western blot electrophoresis equipment and the FlexiVent pulmonary function testing system were purchased from Shanghai Sitek Biological Technology Co., Ltd. Fluorescent primary antibody: recombinant Anti-CD68 antibody (ab53444, Abcam).

Techniques: Expressing

Journal: Scientific Reports

Article Title: HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2—Dectin-1/ SYK mediated ROS and inflammatory responses

doi: 10.1038/s41598-025-13531-0

Figure Lengend Snippet: HSF1 improves lung function and lung injury in asthmatic mice. A: Masson staining of lung tissue changes in the Sham, Model, and Model + HSF1A groups. Scale: 100 μm. B: Immunofluorescence showing CD68 levels in the Sham, Model, and Model + HSF1A groups. Scale: 50 μm. C: Assessment of lung function in mice from the Sham, Model, and Model + HSF1A groups, including FEV1, FVC, FEV1/FVC, FEF 25%, and Ri changes. Data are expressed as mean ± standard deviation. N = 7; Sham vs. Model: * P < 0.05; ** P < 0.01; Model vs. Model + HSF1A: # P < 0.05; ## P < 0.01.

Article Snippet: Macrophage RAW264.7 cells were obtained from the Chinese Cell Resource Center; fetal bovine serum and DMEM medium were purchased from Gibco; ovalbumin (OVA) was obtained from Sigma (USA); LPS was purchased from Sigma; GSH, MDA, IL-1β, and TNFα ELISA kits were purchased from Wuhan Elabscience Biotechnology Co., Ltd.; HSF1 activator HSF1A was obtained from Beijing Biolabs Technology Co., Ltd.; HSF1 inhibitor KRIBB11 was purchased from MCE Company; SIRPα-OE vector and SIRPα shRNA were obtained from Genscript (Nanjing, China); INTERFERin ® kit was purchased from Selleck Company; Masson staining kit was purchased from Beijing Biolabs Technology Co., Ltd.; Western blot electrophoresis equipment and the FlexiVent pulmonary function testing system were purchased from Shanghai Sitek Biological Technology Co., Ltd. Fluorescent primary antibody: recombinant Anti-CD68 antibody (ab53444, Abcam).

Techniques: Staining, Immunofluorescence, Standard Deviation

Journal: Scientific Reports

Article Title: HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2—Dectin-1/ SYK mediated ROS and inflammatory responses

doi: 10.1038/s41598-025-13531-0

Figure Lengend Snippet: HSF1 promotes SIRPα activity and inhibits p-SYK/t-SYK and Dectin-1 function. A: ELISA measuring levels of GSH, MDA, IL-1β, and TNFα in the Sham, Model, and Model + HSF1A groups. B: Western blot analysis of protein expression for P47 (ROS), p-P38/t-P38, p-SIRT1/t-SIRT1, HSF1, SIRPα, p-SYK/t-SYK, and Dectin-1 in the Sham, Model, and Model + HSF1A groups. GAPDH as control protein. Data are expressed as mean ± standard deviation. N = 7; Sham vs. Model: * P < 0.05; ** P < 0.01; Model vs. Model + HSF1A: # P < 0.05; ## P < 0.01.

Article Snippet: Macrophage RAW264.7 cells were obtained from the Chinese Cell Resource Center; fetal bovine serum and DMEM medium were purchased from Gibco; ovalbumin (OVA) was obtained from Sigma (USA); LPS was purchased from Sigma; GSH, MDA, IL-1β, and TNFα ELISA kits were purchased from Wuhan Elabscience Biotechnology Co., Ltd.; HSF1 activator HSF1A was obtained from Beijing Biolabs Technology Co., Ltd.; HSF1 inhibitor KRIBB11 was purchased from MCE Company; SIRPα-OE vector and SIRPα shRNA were obtained from Genscript (Nanjing, China); INTERFERin ® kit was purchased from Selleck Company; Masson staining kit was purchased from Beijing Biolabs Technology Co., Ltd.; Western blot electrophoresis equipment and the FlexiVent pulmonary function testing system were purchased from Shanghai Sitek Biological Technology Co., Ltd. Fluorescent primary antibody: recombinant Anti-CD68 antibody (ab53444, Abcam).

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control, Standard Deviation

Journal: Scientific Reports

Article Title: HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2—Dectin-1/ SYK mediated ROS and inflammatory responses

doi: 10.1038/s41598-025-13531-0

Figure Lengend Snippet: In macrophages, HSF1 mediates ROS and inflammatory responses by regulating the SIRPα/Dectin-1/SYK balance, thereby inhibiting asthma progression. A: Western blot analysis of HSF1, SIRPα, p-SYK/t-SYK, Dectin-1, P22, P47, and p-P38/t-P38 protein expression in the NC, OVA + LPS, HSF1A + OVA + LPS, KRIBB11 + OVA + LPS, and SIRPα-OE + KRIBB11 + OVA + LPS groups. B: ELISA measurements of GSH, MDA, IL-1β, and TNFα levels in the NC, OVA + LPS, HSF1A + OVA + LPS, KRIBB11 + OVA + LPS, and SIRPα-OE + KRIBB11 + OVA + LPS groups. GAPDH as control protein. Data are expressed as mean ± standard deviation. N = 3; NC vs. OVA + LPS: ** P < 0.01; ns P >0.05; OVA + LPS vs. HSF1A + OVA + LPS: # P < 0.05; ## P < 0.01; OVA + LPS vs. KRIBB11 + OVA + LPS: & P < 0.05; && P < 0.01; ns P >0.05; KRIBB11 + OVA + LPS vs. SIRPα + KRIBB11 + OVA + LPS: †† P < 0.01; ns P >0.05.

Article Snippet: Macrophage RAW264.7 cells were obtained from the Chinese Cell Resource Center; fetal bovine serum and DMEM medium were purchased from Gibco; ovalbumin (OVA) was obtained from Sigma (USA); LPS was purchased from Sigma; GSH, MDA, IL-1β, and TNFα ELISA kits were purchased from Wuhan Elabscience Biotechnology Co., Ltd.; HSF1 activator HSF1A was obtained from Beijing Biolabs Technology Co., Ltd.; HSF1 inhibitor KRIBB11 was purchased from MCE Company; SIRPα-OE vector and SIRPα shRNA were obtained from Genscript (Nanjing, China); INTERFERin ® kit was purchased from Selleck Company; Masson staining kit was purchased from Beijing Biolabs Technology Co., Ltd.; Western blot electrophoresis equipment and the FlexiVent pulmonary function testing system were purchased from Shanghai Sitek Biological Technology Co., Ltd. Fluorescent primary antibody: recombinant Anti-CD68 antibody (ab53444, Abcam).

Techniques: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Control, Standard Deviation

Journal: Scientific Reports

Article Title: HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2—Dectin-1/ SYK mediated ROS and inflammatory responses

doi: 10.1038/s41598-025-13531-0

Figure Lengend Snippet: Mechanistic study of how HSF1 inhibits asthma progression through the SIRPα/Dectin-1/SYK balance-mediated ROS and inflammatory responses in macrophages (Created with BioRender.com).

Article Snippet: Macrophage RAW264.7 cells were obtained from the Chinese Cell Resource Center; fetal bovine serum and DMEM medium were purchased from Gibco; ovalbumin (OVA) was obtained from Sigma (USA); LPS was purchased from Sigma; GSH, MDA, IL-1β, and TNFα ELISA kits were purchased from Wuhan Elabscience Biotechnology Co., Ltd.; HSF1 activator HSF1A was obtained from Beijing Biolabs Technology Co., Ltd.; HSF1 inhibitor KRIBB11 was purchased from MCE Company; SIRPα-OE vector and SIRPα shRNA were obtained from Genscript (Nanjing, China); INTERFERin ® kit was purchased from Selleck Company; Masson staining kit was purchased from Beijing Biolabs Technology Co., Ltd.; Western blot electrophoresis equipment and the FlexiVent pulmonary function testing system were purchased from Shanghai Sitek Biological Technology Co., Ltd. Fluorescent primary antibody: recombinant Anti-CD68 antibody (ab53444, Abcam).

Techniques: