hsf1 Search Results


gene exp hsf1 hs01027608 g1  (Thermo Fisher)
85
Thermo Fisher gene exp hsf1 hs01027608 g1
Promoter deletion constructs fused to Luciferase were tested for activity in HeLa, SKOV3 and OvCa429 cells. pRSV-Luc, positive control, 0, empty vector control. Numbers are base pairs relative to the transcriptional start site (0). Bioinformatic analysis (Genomatix) of the most active region within the KIF14 promoter (−2366 to −2245) identified putative binding sites for transcription factors YY1, <t>HSF1</t> and Sp1. Specific transcription factor recognition sites are underlined, and space within sequence denotes location of deletion constructs. N = 3, * Significance at P <0.05, unpaired t-test. P = 0.01 (HeLa); P = 0.01 (SKOV3); P = 0.03 (OvCa429).
Gene Exp Hsf1 Hs01027608 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hsf1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc hsf1
Promoter deletion constructs fused to Luciferase were tested for activity in HeLa, SKOV3 and OvCa429 cells. pRSV-Luc, positive control, 0, empty vector control. Numbers are base pairs relative to the transcriptional start site (0). Bioinformatic analysis (Genomatix) of the most active region within the KIF14 promoter (−2366 to −2245) identified putative binding sites for transcription factors YY1, <t>HSF1</t> and Sp1. Specific transcription factor recognition sites are underlined, and space within sequence denotes location of deletion constructs. N = 3, * Significance at P <0.05, unpaired t-test. P = 0.01 (HeLa); P = 0.01 (SKOV3); P = 0.03 (OvCa429).
Hsf1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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ms2 p65 hsf1 gfp  (Addgene inc)
94
Addgene inc ms2 p65 hsf1 gfp
Promoter deletion constructs fused to Luciferase were tested for activity in HeLa, SKOV3 and OvCa429 cells. pRSV-Luc, positive control, 0, empty vector control. Numbers are base pairs relative to the transcriptional start site (0). Bioinformatic analysis (Genomatix) of the most active region within the KIF14 promoter (−2366 to −2245) identified putative binding sites for transcription factors YY1, <t>HSF1</t> and Sp1. Specific transcription factor recognition sites are underlined, and space within sequence denotes location of deletion constructs. N = 3, * Significance at P <0.05, unpaired t-test. P = 0.01 (HeLa); P = 0.01 (SKOV3); P = 0.03 (OvCa429).
Ms2 P65 Hsf1 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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ms2 p65 hsf1 activator helper complex  (Addgene inc)
93
Addgene inc ms2 p65 hsf1 activator helper complex
Promoter deletion constructs fused to Luciferase were tested for activity in HeLa, SKOV3 and OvCa429 cells. pRSV-Luc, positive control, 0, empty vector control. Numbers are base pairs relative to the transcriptional start site (0). Bioinformatic analysis (Genomatix) of the most active region within the KIF14 promoter (−2366 to −2245) identified putative binding sites for transcription factors YY1, <t>HSF1</t> and Sp1. Specific transcription factor recognition sites are underlined, and space within sequence denotes location of deletion constructs. N = 3, * Significance at P <0.05, unpaired t-test. P = 0.01 (HeLa); P = 0.01 (SKOV3); P = 0.03 (OvCa429).
Ms2 P65 Hsf1 Activator Helper Complex, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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51034 1 ap  (Proteintech)
94
Proteintech 51034 1 ap
Promoter deletion constructs fused to Luciferase were tested for activity in HeLa, SKOV3 and OvCa429 cells. pRSV-Luc, positive control, 0, empty vector control. Numbers are base pairs relative to the transcriptional start site (0). Bioinformatic analysis (Genomatix) of the most active region within the KIF14 promoter (−2366 to −2245) identified putative binding sites for transcription factors YY1, <t>HSF1</t> and Sp1. Specific transcription factor recognition sites are underlined, and space within sequence denotes location of deletion constructs. N = 3, * Significance at P <0.05, unpaired t-test. P = 0.01 (HeLa); P = 0.01 (SKOV3); P = 0.03 (OvCa429).
51034 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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antibodies against hsf1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc antibodies against hsf1
Promoter deletion constructs fused to Luciferase were tested for activity in HeLa, SKOV3 and OvCa429 cells. pRSV-Luc, positive control, 0, empty vector control. Numbers are base pairs relative to the transcriptional start site (0). Bioinformatic analysis (Genomatix) of the most active region within the KIF14 promoter (−2366 to −2245) identified putative binding sites for transcription factors YY1, <t>HSF1</t> and Sp1. Specific transcription factor recognition sites are underlined, and space within sequence denotes location of deletion constructs. N = 3, * Significance at P <0.05, unpaired t-test. P = 0.01 (HeLa); P = 0.01 (SKOV3); P = 0.03 (OvCa429).
Antibodies Against Hsf1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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steroidogenic factor 1 nr5a1 human  (OriGene)
93
OriGene steroidogenic factor 1 nr5a1 human
Promoter deletion constructs fused to Luciferase were tested for activity in HeLa, SKOV3 and OvCa429 cells. pRSV-Luc, positive control, 0, empty vector control. Numbers are base pairs relative to the transcriptional start site (0). Bioinformatic analysis (Genomatix) of the most active region within the KIF14 promoter (−2366 to −2245) identified putative binding sites for transcription factors YY1, <t>HSF1</t> and Sp1. Specific transcription factor recognition sites are underlined, and space within sequence denotes location of deletion constructs. N = 3, * Significance at P <0.05, unpaired t-test. P = 0.01 (HeLa); P = 0.01 (SKOV3); P = 0.03 (OvCa429).
Steroidogenic Factor 1 Nr5a1 Human, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lenti ms2 p65 hsf1 hygro  (Addgene inc)
95
Addgene inc lenti ms2 p65 hsf1 hygro
Promoter deletion constructs fused to Luciferase were tested for activity in HeLa, SKOV3 and OvCa429 cells. pRSV-Luc, positive control, 0, empty vector control. Numbers are base pairs relative to the transcriptional start site (0). Bioinformatic analysis (Genomatix) of the most active region within the KIF14 promoter (−2366 to −2245) identified putative binding sites for transcription factors YY1, <t>HSF1</t> and Sp1. Specific transcription factor recognition sites are underlined, and space within sequence denotes location of deletion constructs. N = 3, * Significance at P <0.05, unpaired t-test. P = 0.01 (HeLa); P = 0.01 (SKOV3); P = 0.03 (OvCa429).
Lenti Ms2 P65 Hsf1 Hygro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mammalian expression vector pcmv6 entry hnr5a1  (OriGene)
91
OriGene mammalian expression vector pcmv6 entry hnr5a1
Promoter deletion constructs fused to Luciferase were tested for activity in HeLa, SKOV3 and OvCa429 cells. pRSV-Luc, positive control, 0, empty vector control. Numbers are base pairs relative to the transcriptional start site (0). Bioinformatic analysis (Genomatix) of the most active region within the KIF14 promoter (−2366 to −2245) identified putative binding sites for transcription factors YY1, <t>HSF1</t> and Sp1. Specific transcription factor recognition sites are underlined, and space within sequence denotes location of deletion constructs. N = 3, * Significance at P <0.05, unpaired t-test. P = 0.01 (HeLa); P = 0.01 (SKOV3); P = 0.03 (OvCa429).
Mammalian Expression Vector Pcmv6 Entry Hnr5a1, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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transcription factor hsf1  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology transcription factor hsf1
Promoter deletion constructs fused to Luciferase were tested for activity in HeLa, SKOV3 and OvCa429 cells. pRSV-Luc, positive control, 0, empty vector control. Numbers are base pairs relative to the transcriptional start site (0). Bioinformatic analysis (Genomatix) of the most active region within the KIF14 promoter (−2366 to −2245) identified putative binding sites for transcription factors YY1, <t>HSF1</t> and Sp1. Specific transcription factor recognition sites are underlined, and space within sequence denotes location of deletion constructs. N = 3, * Significance at P <0.05, unpaired t-test. P = 0.01 (HeLa); P = 0.01 (SKOV3); P = 0.03 (OvCa429).
Transcription Factor Hsf1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hsf1  (Bethyl)
92
Bethyl hsf1
(A and B) p53 and <t>HSF1</t> protein levels in Q111 cells relative to Q7 (n = 5).
Hsf1, supplied by Bethyl, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hsf1 flag  (Addgene inc)
88
Addgene inc hsf1 flag
(A and B) p53 and <t>HSF1</t> protein levels in Q111 cells relative to Q7 (n = 5).
Hsf1 Flag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Promoter deletion constructs fused to Luciferase were tested for activity in HeLa, SKOV3 and OvCa429 cells. pRSV-Luc, positive control, 0, empty vector control. Numbers are base pairs relative to the transcriptional start site (0). Bioinformatic analysis (Genomatix) of the most active region within the KIF14 promoter (−2366 to −2245) identified putative binding sites for transcription factors YY1, HSF1 and Sp1. Specific transcription factor recognition sites are underlined, and space within sequence denotes location of deletion constructs. N = 3, * Significance at P <0.05, unpaired t-test. P = 0.01 (HeLa); P = 0.01 (SKOV3); P = 0.03 (OvCa429).

Journal: PLoS ONE

Article Title: Transcriptional and Epigenetic Regulation of KIF14 Overexpression in Ovarian Cancer

doi: 10.1371/journal.pone.0091540

Figure Lengend Snippet: Promoter deletion constructs fused to Luciferase were tested for activity in HeLa, SKOV3 and OvCa429 cells. pRSV-Luc, positive control, 0, empty vector control. Numbers are base pairs relative to the transcriptional start site (0). Bioinformatic analysis (Genomatix) of the most active region within the KIF14 promoter (−2366 to −2245) identified putative binding sites for transcription factors YY1, HSF1 and Sp1. Specific transcription factor recognition sites are underlined, and space within sequence denotes location of deletion constructs. N = 3, * Significance at P <0.05, unpaired t-test. P = 0.01 (HeLa); P = 0.01 (SKOV3); P = 0.03 (OvCa429).

Article Snippet: 1 μL of synthesized cDNA was added to 1X TaqMan PCR master mix (ABI), and 1X TaqMan Gene Expression Assay primer-probe mix for KIF14 (Hs00978216_m1), Sp1 (Hs00916521_m1), YY1 (Hs00231533_m1), and HSF1 (Hs01027608_g1).

Techniques: Construct, Luciferase, Activity Assay, Positive Control, Plasmid Preparation, Control, Binding Assay, Sequencing

siRNA knockdown of endogenous Sp1 (orange), HSF1 (green), and YY1 (red) transcription factors, and measurement of their mRNA expression along with corresponding KIF14 mRNA levels (blue) via real-time PCR in A SKOV3 and B OvCa429 cells. Y-axes: normalized mRNA expression relative to MOCK. GL2, control siRNA; N = 3, * Significance at P <0.05, unpaired t-test. Three different siRNA molecules (A, B, C) were used to knock down each gene. P values for panel A (SKOV3 cells): P = 0.02 for Sp1 expression (orange) with Sp1 siRNAs A, B, and C; P = 0.03 (siRNA-A), P = 0.047 (siRNA–B), P = 0.04 (siRNA–C) for KIF14 expression (blue). P = 0.001 for HSF1 expression (green) with HSF1 siRNAs A, B, and C; P = 0.23 (siRNA-A), P = 0.12 (siRNA-B), P = 0.4 (siRNA-C) for KIF14 expression (blue). P = 0.01 for YY1 expression (red) with YY1 siRNAs A, B and C; P = 0.006 for KIF14 expression (blue) with YY1 siRNAs A, B, and C. P values for panel B (OvCa429 cells): P = 0.03 for Sp1 expression (orange) with Sp1 siRNAs A, B, and C; P = 0.05 (siRNA-A), P = 0.04 (siRNA-B), P = 0.045 (siRNA-C) for KIF14 expression (blue). P = 0.003 for HSF1 expression (green) with HSF1 siRNAs A, B, and C; P = 0.31 (siRNA-A), P = 0.45 (siRNA-B), P = 0.39 (siRNA-C) for KIF14 expression (blue). P = 0.02 (siRNA-A), P = 0.01 (siRNA-B), P = 0.01 for YY1 expression (red); P = 0.02 (siRNA-A), P = 0.001 (siRNA-B), P = 0.006 (siRNA-C) for KIF14 expression (blue).

Journal: PLoS ONE

Article Title: Transcriptional and Epigenetic Regulation of KIF14 Overexpression in Ovarian Cancer

doi: 10.1371/journal.pone.0091540

Figure Lengend Snippet: siRNA knockdown of endogenous Sp1 (orange), HSF1 (green), and YY1 (red) transcription factors, and measurement of their mRNA expression along with corresponding KIF14 mRNA levels (blue) via real-time PCR in A SKOV3 and B OvCa429 cells. Y-axes: normalized mRNA expression relative to MOCK. GL2, control siRNA; N = 3, * Significance at P <0.05, unpaired t-test. Three different siRNA molecules (A, B, C) were used to knock down each gene. P values for panel A (SKOV3 cells): P = 0.02 for Sp1 expression (orange) with Sp1 siRNAs A, B, and C; P = 0.03 (siRNA-A), P = 0.047 (siRNA–B), P = 0.04 (siRNA–C) for KIF14 expression (blue). P = 0.001 for HSF1 expression (green) with HSF1 siRNAs A, B, and C; P = 0.23 (siRNA-A), P = 0.12 (siRNA-B), P = 0.4 (siRNA-C) for KIF14 expression (blue). P = 0.01 for YY1 expression (red) with YY1 siRNAs A, B and C; P = 0.006 for KIF14 expression (blue) with YY1 siRNAs A, B, and C. P values for panel B (OvCa429 cells): P = 0.03 for Sp1 expression (orange) with Sp1 siRNAs A, B, and C; P = 0.05 (siRNA-A), P = 0.04 (siRNA-B), P = 0.045 (siRNA-C) for KIF14 expression (blue). P = 0.003 for HSF1 expression (green) with HSF1 siRNAs A, B, and C; P = 0.31 (siRNA-A), P = 0.45 (siRNA-B), P = 0.39 (siRNA-C) for KIF14 expression (blue). P = 0.02 (siRNA-A), P = 0.01 (siRNA-B), P = 0.01 for YY1 expression (red); P = 0.02 (siRNA-A), P = 0.001 (siRNA-B), P = 0.006 (siRNA-C) for KIF14 expression (blue).

Article Snippet: 1 μL of synthesized cDNA was added to 1X TaqMan PCR master mix (ABI), and 1X TaqMan Gene Expression Assay primer-probe mix for KIF14 (Hs00978216_m1), Sp1 (Hs00916521_m1), YY1 (Hs00231533_m1), and HSF1 (Hs01027608_g1).

Techniques: Knockdown, Expressing, Real-time Polymerase Chain Reaction, Control

A siRNA knockdown of endogenous Sp1 (orange), HSF1 (green), and YY1 (red) transcription factors, and measurement of their protein expression along with corresponding KIF14 protein levels (blue) via immunoblot in SKOV3 cells. x-axis: normalized protein expression relative to MOCK. B Representative immunoblot of KIF14 and transcription factor expression. Numbers represent normalized expression values for the experiment shown. Similar results were seen with OvCa429 cells. GL2, control siRNA; N = 3; *, P <0.05 for transcription factor expression; #, P <0.05 for KIF14 expression, unpaired t-test. P values for panel A (SKOV3 cells): P = 0.009 (siRNA-A), P = 0.003 (siRNA-B), P = 0.006 (siRNA-C) for Sp1 expression (orange); P = 0.005 (siRNA-A), P = 0.007 (siRNA-B), P = 0.004 (siRNA-C) for KIF14 expression (blue). P = 0.01 for HSF1 expression (green) with HSF1 siRNAs A, B, and C; P = 0.54 (siRNA-A), P = 0.65 (siRNA-B), P = 0.41 (siRNA-C) for KIF14 expression (blue). P = 0.001 for YY1 expression (red) with YY1 siRNAs A, B and C; P = 0.01 (siRNA-A), P = 0.02 (siRNA-B), P = 0.005 (siRNA-C) for KIF14 expression (blue).

Journal: PLoS ONE

Article Title: Transcriptional and Epigenetic Regulation of KIF14 Overexpression in Ovarian Cancer

doi: 10.1371/journal.pone.0091540

Figure Lengend Snippet: A siRNA knockdown of endogenous Sp1 (orange), HSF1 (green), and YY1 (red) transcription factors, and measurement of their protein expression along with corresponding KIF14 protein levels (blue) via immunoblot in SKOV3 cells. x-axis: normalized protein expression relative to MOCK. B Representative immunoblot of KIF14 and transcription factor expression. Numbers represent normalized expression values for the experiment shown. Similar results were seen with OvCa429 cells. GL2, control siRNA; N = 3; *, P <0.05 for transcription factor expression; #, P <0.05 for KIF14 expression, unpaired t-test. P values for panel A (SKOV3 cells): P = 0.009 (siRNA-A), P = 0.003 (siRNA-B), P = 0.006 (siRNA-C) for Sp1 expression (orange); P = 0.005 (siRNA-A), P = 0.007 (siRNA-B), P = 0.004 (siRNA-C) for KIF14 expression (blue). P = 0.01 for HSF1 expression (green) with HSF1 siRNAs A, B, and C; P = 0.54 (siRNA-A), P = 0.65 (siRNA-B), P = 0.41 (siRNA-C) for KIF14 expression (blue). P = 0.001 for YY1 expression (red) with YY1 siRNAs A, B and C; P = 0.01 (siRNA-A), P = 0.02 (siRNA-B), P = 0.005 (siRNA-C) for KIF14 expression (blue).

Article Snippet: 1 μL of synthesized cDNA was added to 1X TaqMan PCR master mix (ABI), and 1X TaqMan Gene Expression Assay primer-probe mix for KIF14 (Hs00978216_m1), Sp1 (Hs00916521_m1), YY1 (Hs00231533_m1), and HSF1 (Hs01027608_g1).

Techniques: Knockdown, Expressing, Western Blot, Control

ChIP assays of endogenous YY1, Sp1 and HSF1 followed by real time PCR with the KIF14 promoter region (−2300 to −2133) in cell lines SKOV3, OvCa429, and HeLa compared to IgG (negative control). Values represent average quantity of promoter region product relative to IgG control. Error bars represent standard deviation of triplicate assays.

Journal: PLoS ONE

Article Title: Transcriptional and Epigenetic Regulation of KIF14 Overexpression in Ovarian Cancer

doi: 10.1371/journal.pone.0091540

Figure Lengend Snippet: ChIP assays of endogenous YY1, Sp1 and HSF1 followed by real time PCR with the KIF14 promoter region (−2300 to −2133) in cell lines SKOV3, OvCa429, and HeLa compared to IgG (negative control). Values represent average quantity of promoter region product relative to IgG control. Error bars represent standard deviation of triplicate assays.

Article Snippet: 1 μL of synthesized cDNA was added to 1X TaqMan PCR master mix (ABI), and 1X TaqMan Gene Expression Assay primer-probe mix for KIF14 (Hs00978216_m1), Sp1 (Hs00916521_m1), YY1 (Hs00231533_m1), and HSF1 (Hs01027608_g1).

Techniques: Real-time Polymerase Chain Reaction, Negative Control, Control, Standard Deviation

Quantitative mRNA expression analysis of primary OvCa tumors with KIF14 HIGH (red) and KIF14 LOW (green) mRNA expression (but no KIF14 genomic gain) for Sp1 (circle), YY1 (triangle), and HSF1 (diamond) normalized to normal ovary expression (set as 1, black dashed line). Mean, black line. Individual tumors represented by symbols. * Significance at P <0.05, paired t-test. P = 0.03 (Sp1), P = 0.01 (YY1), P = 0.32 (HSF1).

Journal: PLoS ONE

Article Title: Transcriptional and Epigenetic Regulation of KIF14 Overexpression in Ovarian Cancer

doi: 10.1371/journal.pone.0091540

Figure Lengend Snippet: Quantitative mRNA expression analysis of primary OvCa tumors with KIF14 HIGH (red) and KIF14 LOW (green) mRNA expression (but no KIF14 genomic gain) for Sp1 (circle), YY1 (triangle), and HSF1 (diamond) normalized to normal ovary expression (set as 1, black dashed line). Mean, black line. Individual tumors represented by symbols. * Significance at P <0.05, paired t-test. P = 0.03 (Sp1), P = 0.01 (YY1), P = 0.32 (HSF1).

Article Snippet: 1 μL of synthesized cDNA was added to 1X TaqMan PCR master mix (ABI), and 1X TaqMan Gene Expression Assay primer-probe mix for KIF14 (Hs00978216_m1), Sp1 (Hs00916521_m1), YY1 (Hs00231533_m1), and HSF1 (Hs01027608_g1).

Techniques: Expressing

Pearson correlation between KIF14 gain/no gain tumors and Sp1 , HSF1 and YY1 mRNA.

Journal: PLoS ONE

Article Title: Transcriptional and Epigenetic Regulation of KIF14 Overexpression in Ovarian Cancer

doi: 10.1371/journal.pone.0091540

Figure Lengend Snippet: Pearson correlation between KIF14 gain/no gain tumors and Sp1 , HSF1 and YY1 mRNA.

Article Snippet: 1 μL of synthesized cDNA was added to 1X TaqMan PCR master mix (ABI), and 1X TaqMan Gene Expression Assay primer-probe mix for KIF14 (Hs00978216_m1), Sp1 (Hs00916521_m1), YY1 (Hs00231533_m1), and HSF1 (Hs01027608_g1).

Techniques:

(A and B) p53 and HSF1 protein levels in Q111 cells relative to Q7 (n = 5).

Journal: Cell reports

Article Title: Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington’s disease

doi: 10.1016/j.celrep.2023.112198

Figure Lengend Snippet: (A and B) p53 and HSF1 protein levels in Q111 cells relative to Q7 (n = 5).

Article Snippet: Primary antibodies are listed in the and in Table S1 . anti-CK2α’ (Novus NB100-379, RRID:AB_100000802 and Proteintech 10606-1-AP, RRID:AB_2292447), HTT (Millipore, Mab5374 clone EM48, RRID:AB_10055116), HTT (Abcam, Mabab109115, RID:AB_10863082), HSF1 (Bethyl antibodies A303-176A, RRID:AB_10892628), p53 (Santa Cruz, clone DO1 SC-126, RRID: AB_628082), MDM2 (Santa Cruz sc-965, RRID:AB_627920), MDM4 (Aviva Systems Biology AVARP06007_T100, RRID:AB_841544), aB-crys (Enzo, ADI-SPA-223D, RRID:AB_2039025), FBXW7 (Abcam, Ab109617 , RRID:AB_2687519) GAPDH (Santacruz sc-365062, RRID:AB_10847862), Actin (Santa Cruz sc-8432, RRID:AB_626630), H3 (Cell Signaling technology, 9715, RRID:AB_331563), FLAG (Santa Cruz, sc-166355, RRID:AB_2017593), GFP (Santa Cruz, sc-9996, RRID:AB_627695).

Techniques:

(A and B) HSF1 and p53 protein levels in Q7 and Q111 non-transfected cells (−) and Q111 treated cells with scramble (scr.) or sip53 (n = 3).

Journal: Cell reports

Article Title: Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington’s disease

doi: 10.1016/j.celrep.2023.112198

Figure Lengend Snippet: (A and B) HSF1 and p53 protein levels in Q7 and Q111 non-transfected cells (−) and Q111 treated cells with scramble (scr.) or sip53 (n = 3).

Article Snippet: Primary antibodies are listed in the and in Table S1 . anti-CK2α’ (Novus NB100-379, RRID:AB_100000802 and Proteintech 10606-1-AP, RRID:AB_2292447), HTT (Millipore, Mab5374 clone EM48, RRID:AB_10055116), HTT (Abcam, Mabab109115, RID:AB_10863082), HSF1 (Bethyl antibodies A303-176A, RRID:AB_10892628), p53 (Santa Cruz, clone DO1 SC-126, RRID: AB_628082), MDM2 (Santa Cruz sc-965, RRID:AB_627920), MDM4 (Aviva Systems Biology AVARP06007_T100, RRID:AB_841544), aB-crys (Enzo, ADI-SPA-223D, RRID:AB_2039025), FBXW7 (Abcam, Ab109617 , RRID:AB_2687519) GAPDH (Santacruz sc-365062, RRID:AB_10847862), Actin (Santa Cruz sc-8432, RRID:AB_626630), H3 (Cell Signaling technology, 9715, RRID:AB_331563), FLAG (Santa Cruz, sc-166355, RRID:AB_2017593), GFP (Santa Cruz, sc-9996, RRID:AB_627695).

Techniques: Transfection

(A) Immunoblotting in cytoplasm and nuclear fractions of Q7 and Q111 cells treated cells with scr. or sip53. HSF1 is shown at two different exposures (long and short).

Journal: Cell reports

Article Title: Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington’s disease

doi: 10.1016/j.celrep.2023.112198

Figure Lengend Snippet: (A) Immunoblotting in cytoplasm and nuclear fractions of Q7 and Q111 cells treated cells with scr. or sip53. HSF1 is shown at two different exposures (long and short).

Article Snippet: Primary antibodies are listed in the and in Table S1 . anti-CK2α’ (Novus NB100-379, RRID:AB_100000802 and Proteintech 10606-1-AP, RRID:AB_2292447), HTT (Millipore, Mab5374 clone EM48, RRID:AB_10055116), HTT (Abcam, Mabab109115, RID:AB_10863082), HSF1 (Bethyl antibodies A303-176A, RRID:AB_10892628), p53 (Santa Cruz, clone DO1 SC-126, RRID: AB_628082), MDM2 (Santa Cruz sc-965, RRID:AB_627920), MDM4 (Aviva Systems Biology AVARP06007_T100, RRID:AB_841544), aB-crys (Enzo, ADI-SPA-223D, RRID:AB_2039025), FBXW7 (Abcam, Ab109617 , RRID:AB_2687519) GAPDH (Santacruz sc-365062, RRID:AB_10847862), Actin (Santa Cruz sc-8432, RRID:AB_626630), H3 (Cell Signaling technology, 9715, RRID:AB_331563), FLAG (Santa Cruz, sc-166355, RRID:AB_2017593), GFP (Santa Cruz, sc-9996, RRID:AB_627695).

Techniques: Western Blot

(A and B) p53, HSF1, and DARPP-32 protein levels in striatum samples from 12-month-old WT and zQ175 mice (n = 3).

Journal: Cell reports

Article Title: Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington’s disease

doi: 10.1016/j.celrep.2023.112198

Figure Lengend Snippet: (A and B) p53, HSF1, and DARPP-32 protein levels in striatum samples from 12-month-old WT and zQ175 mice (n = 3).

Article Snippet: Primary antibodies are listed in the and in Table S1 . anti-CK2α’ (Novus NB100-379, RRID:AB_100000802 and Proteintech 10606-1-AP, RRID:AB_2292447), HTT (Millipore, Mab5374 clone EM48, RRID:AB_10055116), HTT (Abcam, Mabab109115, RID:AB_10863082), HSF1 (Bethyl antibodies A303-176A, RRID:AB_10892628), p53 (Santa Cruz, clone DO1 SC-126, RRID: AB_628082), MDM2 (Santa Cruz sc-965, RRID:AB_627920), MDM4 (Aviva Systems Biology AVARP06007_T100, RRID:AB_841544), aB-crys (Enzo, ADI-SPA-223D, RRID:AB_2039025), FBXW7 (Abcam, Ab109617 , RRID:AB_2687519) GAPDH (Santacruz sc-365062, RRID:AB_10847862), Actin (Santa Cruz sc-8432, RRID:AB_626630), H3 (Cell Signaling technology, 9715, RRID:AB_331563), FLAG (Santa Cruz, sc-166355, RRID:AB_2017593), GFP (Santa Cruz, sc-9996, RRID:AB_627695).

Techniques:

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington’s disease

doi: 10.1016/j.celrep.2023.112198

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Primary antibodies are listed in the and in Table S1 . anti-CK2α’ (Novus NB100-379, RRID:AB_100000802 and Proteintech 10606-1-AP, RRID:AB_2292447), HTT (Millipore, Mab5374 clone EM48, RRID:AB_10055116), HTT (Abcam, Mabab109115, RID:AB_10863082), HSF1 (Bethyl antibodies A303-176A, RRID:AB_10892628), p53 (Santa Cruz, clone DO1 SC-126, RRID: AB_628082), MDM2 (Santa Cruz sc-965, RRID:AB_627920), MDM4 (Aviva Systems Biology AVARP06007_T100, RRID:AB_841544), aB-crys (Enzo, ADI-SPA-223D, RRID:AB_2039025), FBXW7 (Abcam, Ab109617 , RRID:AB_2687519) GAPDH (Santacruz sc-365062, RRID:AB_10847862), Actin (Santa Cruz sc-8432, RRID:AB_626630), H3 (Cell Signaling technology, 9715, RRID:AB_331563), FLAG (Santa Cruz, sc-166355, RRID:AB_2017593), GFP (Santa Cruz, sc-9996, RRID:AB_627695).

Techniques: Control, Recombinant, Modification, Purification, Extraction, Enzyme-linked Immunosorbent Assay, Mutagenesis, Software, Binding Assay