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human peripheral blood mononuclear cells pbmcs  (ATCC)


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    ATCC human peripheral blood mononuclear cells pbmcs
    Comparative induction of T cell exhaustion markers in colorectal cancer cell lines upon PBMC co-culture. Bar graph showing relative expression (fold change vs. monoculture baseline) of PDCD1, LAG3, HAVCR2, and CD27 in MSI-H cell lines (HCT116 and RKO) and MSS cell lines (HCT15 and SW480) following 48-h co-culture with activated <t>PBMCs.</t> Gray dashed line represents monoculture baseline (fold change = 1). MSI-H cell lines consistently demonstrated 2-3 times higher induction of all four exhaustion markers compared to MSS cell lines, with PDCD1 and LAG3 showing the greatest differential responses. Data represent mean values from three independent biological replicates analyzed by qRT-PCR with GAPDH normalization using the 2 -ΔΔCt method.
    Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 878 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hpbmcs/Primary+Peripheral+Blood+Mononuclear+Cells+(PBMC)%2C+Normal%2C+Human/pmc13258542-88-0-11
    Average 99 stars, based on 878 article reviews
    human peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2026-10
    99/100 stars

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    1) Product Images from "Single-cell transcriptomic profiling reveals CD27 + cytotoxic T Cell heterogeneity and exhaustion dynamics in colorectal cancer tumor microenvironment"

    Article Title: Single-cell transcriptomic profiling reveals CD27 + cytotoxic T Cell heterogeneity and exhaustion dynamics in colorectal cancer tumor microenvironment

    Journal: Frontiers in Genetics

    doi: 10.3389/fgene.2026.1808171

    Comparative induction of T cell exhaustion markers in colorectal cancer cell lines upon PBMC co-culture. Bar graph showing relative expression (fold change vs. monoculture baseline) of PDCD1, LAG3, HAVCR2, and CD27 in MSI-H cell lines (HCT116 and RKO) and MSS cell lines (HCT15 and SW480) following 48-h co-culture with activated PBMCs. Gray dashed line represents monoculture baseline (fold change = 1). MSI-H cell lines consistently demonstrated 2-3 times higher induction of all four exhaustion markers compared to MSS cell lines, with PDCD1 and LAG3 showing the greatest differential responses. Data represent mean values from three independent biological replicates analyzed by qRT-PCR with GAPDH normalization using the 2 -ΔΔCt method.
    Figure Legend Snippet: Comparative induction of T cell exhaustion markers in colorectal cancer cell lines upon PBMC co-culture. Bar graph showing relative expression (fold change vs. monoculture baseline) of PDCD1, LAG3, HAVCR2, and CD27 in MSI-H cell lines (HCT116 and RKO) and MSS cell lines (HCT15 and SW480) following 48-h co-culture with activated PBMCs. Gray dashed line represents monoculture baseline (fold change = 1). MSI-H cell lines consistently demonstrated 2-3 times higher induction of all four exhaustion markers compared to MSS cell lines, with PDCD1 and LAG3 showing the greatest differential responses. Data represent mean values from three independent biological replicates analyzed by qRT-PCR with GAPDH normalization using the 2 -ΔΔCt method.

    Techniques Used: Co-Culture Assay, Expressing, Quantitative RT-PCR

    Related Articles

    Injection:

    Article Title: CD8a antibody-functionalized biomimetic red blood cell membrane ectosomes delivering C646 reverse CD8⁺ T Cell exhaustion via H3K18la histone delactylation in gastric cardia adenocarcinoma
    Article Snippet: NOD SCID immunodeficient mice (4–5 weeks old, 18–22 g; C001070, Cyagen, Jiangsu, China) were acclimated for one week before experimentation. .. Humanized immune system models were established by intraperitoneal injection of hPBMCs (PCS-800–011, ATCC, USA), leading to immune cell reconstitution within two weeks [ ]. .. Mice were anesthetized with 3% isoflurane (792,632, Sigma-Aldrich, USA), and 5 × 107 MKN-45 cells were injected subcutaneously into the ventral region.



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    Comparative induction of T cell exhaustion markers in colorectal cancer cell lines upon PBMC co-culture. Bar graph showing relative expression (fold change vs. monoculture baseline) of PDCD1, LAG3, HAVCR2, and CD27 in MSI-H cell lines (HCT116 and RKO) and MSS cell lines (HCT15 and SW480) following 48-h co-culture with activated <t>PBMCs.</t> Gray dashed line represents monoculture baseline (fold change = 1). MSI-H cell lines consistently demonstrated 2-3 times higher induction of all four exhaustion markers compared to MSS cell lines, with PDCD1 and LAG3 showing the greatest differential responses. Data represent mean values from three independent biological replicates analyzed by qRT-PCR with GAPDH normalization using the 2 -ΔΔCt method.
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    Image Search Results


    Comparative induction of T cell exhaustion markers in colorectal cancer cell lines upon PBMC co-culture. Bar graph showing relative expression (fold change vs. monoculture baseline) of PDCD1, LAG3, HAVCR2, and CD27 in MSI-H cell lines (HCT116 and RKO) and MSS cell lines (HCT15 and SW480) following 48-h co-culture with activated PBMCs. Gray dashed line represents monoculture baseline (fold change = 1). MSI-H cell lines consistently demonstrated 2-3 times higher induction of all four exhaustion markers compared to MSS cell lines, with PDCD1 and LAG3 showing the greatest differential responses. Data represent mean values from three independent biological replicates analyzed by qRT-PCR with GAPDH normalization using the 2 -ΔΔCt method.

    Journal: Frontiers in Genetics

    Article Title: Single-cell transcriptomic profiling reveals CD27 + cytotoxic T Cell heterogeneity and exhaustion dynamics in colorectal cancer tumor microenvironment

    doi: 10.3389/fgene.2026.1808171

    Figure Lengend Snippet: Comparative induction of T cell exhaustion markers in colorectal cancer cell lines upon PBMC co-culture. Bar graph showing relative expression (fold change vs. monoculture baseline) of PDCD1, LAG3, HAVCR2, and CD27 in MSI-H cell lines (HCT116 and RKO) and MSS cell lines (HCT15 and SW480) following 48-h co-culture with activated PBMCs. Gray dashed line represents monoculture baseline (fold change = 1). MSI-H cell lines consistently demonstrated 2-3 times higher induction of all four exhaustion markers compared to MSS cell lines, with PDCD1 and LAG3 showing the greatest differential responses. Data represent mean values from three independent biological replicates analyzed by qRT-PCR with GAPDH normalization using the 2 -ΔΔCt method.

    Article Snippet: Human peripheral blood mononuclear cells (PBMCs) were obtained commercially from the American Type Culture Collection (ATCC; catalog no. PCS-800-011) and used in accordance with the supplier’s guidelines.

    Techniques: Co-Culture Assay, Expressing, Quantitative RT-PCR