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Quantitative Difference in PLZF Protein Expression Determines i NKT Lineage Fate and Controls Innate CD8 T Cell Generation

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"Pandolfi (Harvard Medical School, Boston, MA) Oligonucleotides RT-PCR Zbtb16 -Forward: 5′-TTGGGACGGACCCACACCCA-3′ This paper N/A RT-PCR Zbtb16 -Reverse: 5′-GGCGTGGCCGGTGAATAGGG-3′ This paper N/A RT-PCR Rpl13 -Forward: 5′-CGAGGCATGCTGCCCCACAA-3′ This paper N/A RT-PCR Rpl13 -Reverse: 5′-AGCAGGGACCACCATCCGCT-3′ This paper N/A Genotype Zbtb16 WT Forward: 5′-GCAGATCTGGGACCACCATC-3′ This paper N/A Genotype Zbtb16 WT Reverse: 5′-GCTGCATACAGCAGGTCATC-3′ This paper N/A Genotype Zbtb16 KO Forward: 5′- GGTGCCAATGTCACTTATAGAACC-3′ This paper N/A Genotype Zbtb16 KO Reverse: 5′- GCTTCCTCGTGCTTTACGGTATC-3′ This paper N/A Genotype PLZF GFP/CRE WT Forward: 5′-CTCCTCCATGCAGAAACACA-3′ This paper N/A Genotype PLZF GFP/CRE Reverse: 5′-TAGTGAAACAGGGGCAATGG-3′ This paper N/A Genotype PLZF GFP/CRE Rep Forward: 5′-CCCCAGGAAATAATCCAAGG-3′ This paper N/A Software and Algorithms GraphPad Prism 7 GraphPad software N/A FlowJo software version 10.2 FlowJo N/A Active Control 4.2.0.7 Division of Computer Research and Technology, NIH N/A Other Canvas X Canvas GFX N/A BD FACS LSRII BD Biosciences N/A BD FACS LSRFortessa BD Biosciences N/A Open in a separate window KEY RESOURCES TABLE"

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